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culture media Options for Growth and Morphological Characterisation of Cercospora coffeicola Affecting Coffee in Zimbabwe

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NASS Journal of Agricultural Sciences 2023年第1期5卷 19-27页

作者： Mwatsiya.N Mahoya.C Chidoko.P Kutywayo.D Makuvara Z Coffee Research Institute P O Box 6ChipingeZimbabwe Great Zimbabwe University P.O Box 1235MasvingoZimbabwe Division of Crops Research Department of Research and Specialist ServicesP.O.Box 594CausewayHarareZimbabwe

Cercospora leaf spot is fast turning into a critically important disease in *** disease is caused by Cercospora coffeicola which significantly reduces productivity and quality of ***,optimum sporulation of Cercospora coffeicola in culture remains a limiting factor for microbial analysis and quantitative studies of Cercospora leaf *** with this challenge,an in-vitro study was conducted at Coffee Research Institute,Manicaland,Zimbabwe to examine growth of Cercospora coffeicola in different nutrient media and to determine the best media for Cercospora coffeicola *** nutrient media were assessed(corn meal agar,oat meal agar,Czapek Dox agar,malt extract agar,yeast extract agar and potato dextrose agar)for the growth of Cercospora *** laboratory-based experiment was duplicated,laid out in a Completely Randomized Design,replicated three times and based on Cercospora coffeicola nutrient *** were collected on radial growth,colour and texture of mycelium at 3 and 6 days after *** were significant differences(pmedia after 3 and 6 *** extract agar had the greatest radial growth(34 mm and 32 mm)of Cercospora coffeicola for trials 1 and 2 respectively,whilst the least growth was in the oat meal agar(14.2 mm and 15.7 mm)for trials 1 and 2 *** were variations in colour and texture of mycelium with malt extract agar,potato dextrose agar and oat meal agar associated with darker colours and rough texture while smooth white mycelia were found in corn meal *** considering all nutrient media,malt extract agar was found to be the best media for the growth of Cercospora coffeicola *** the basis of our findings,the authors recommend the use of malt extract agar as the primary media for identification and characterisation of Cercospora coffeicola.

关键词： culture media Agar Inoculation Mycelia Cercospora leaf spot Coffee

Methionine-dependence and combination chemotherapy on human gastric cancer cells in vitro

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World Journal of Gastroenterology 2002年第2期8卷 230-232页

作者： Wei-Xin Cao Jing-Min Ou Xu-Feng Fei,Department of Clinical Nutrition,Shanghai Institute of Digestive Surgery,Ruijin Hospital,Shanghai Second Medical University,Shanghai 200025,China Zheng-Gang Zhu Hao-Ran Yin Min Yan Yan-Zhen Lin,Department of Surgery,Shanghai Institute of Digestive Surgery,Ruijin Hospital,Shanghai Second Medical University,Shangha 200025,China Department of Clinical Nutrition Ruijin Hospital Shanghai Second Medical University 197 Ruijiner Road Shanghai 200025 China. fische@***

AIM: To elucidate whether human primary gastric cancer and gastric mucosa epithelial cells in vitro can grow normally in a methionine (Met) depleted environment, i.e. Met-dependence, and whether Met-depleting status can enhance the killing effect of chemotherapy on gastric cancer cells. METHODS: Fresh human gastric cancer and mucosal tissues were managed to form monocellular suspensions, which were then cultured in the Met-free but homocysteine-containing (Met(-)Hcy(+)) medium, with different chemotherapeutic drugs. The proliferation of the cells was examined by cell counter, flow cytometry (FCM) and microcytotoxicity assay (MTT). RESULTS: The growth of human primary gastric cancer cells in Met(-)Hcy(+) was suppressed, manifested by the decrease of total cell counts [1.46 +/- 0.42 (x 10(9).L(-1)) in Met(-)Hcy(+) vs 1.64 +/-0.44(x 10(9).L(-1)) in Met(+)Hcy(-), P<0.01], the decline in the percentage of G(0)G(1) phase cells (0.69 +/- 0.24 in Met(-)Hcy(+) vs 0.80 +/- 0.18 in Met(+)Hcy(-), P<0.01) and the increase of S cells (0.24 +/- 0.20 in Met(-)Hcy(+) vs 0.17 +/- 0.16 in Met(+)Hcy(-), P<0.01); however, gastric mucosal cells grew normally. If Met(-)Hcy(+) medium was used in combination with chemotherapeutic drugs, the number of surviving gastric cancer cells dropped significantly. CONCLUSION: Human primary gastric cancer cells in vitro are Met-dependent; however, gastric mucosal cells have not shown the same characteristics. Met(-)Hcy(+) environment may strengthen the killing effect of chemotherapy on human primary gastric cancer cells.

关键词： Antineoplastic Combined Chemotherapy Protocols Cells, cultured culture media Epithelial Cells Gastric Mucosa Humans Methionine Research Support, Non-U.S. Gov't Stomach Neoplasms

Influencing factors of rat small intestinal epithelial cell cultivation and effects of radiation on cell proliferation

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World Journal of Gastroenterology 2001年第1期7卷 140-142页

作者： Xin Ze Ran Yong Ping Su Yong Jiang Wei Guo Ping Ai Tian Min Cheng Yuan Lin Institute of Combined Injury, PLA, School of Preventive Medicine, Third Military Medical University. Chongqing 400038, China Institute of Combined Injury PLA School of Preventive Medicine Third Military Medical University Chongqing 400038 China. rxz@***

INTRODUCTIONCrypt epithelial cells in normal small intestineproliferate at a high speed. But they are verydifficult to culture in vitro and passage stably. A lotof studies have been done[1-16]. Some domestic labsisolated and cultured crypt cells from embryonalintestines and aseptic animal intestine, but *** introduced normal rat epithelial cell line-IEC-6from the USA and its living condition for stablepassage was successfully established after trials. Thecell line was testified to be the small intestinalepithelial cell by electron microscopy,immunihistochemistry and enzymatic histoch-emistry. It has been applied to some relatedresearch work[17-21]. It was found that manyfactors were involved in the culture system. Ourpresent study focuses on the culture method and theinfluencing factors on IEC-6.

关键词： Animals Blood Proteins Cell Count Cell culture Techniques Cell Division Cell Line culture media Dose-Response Relationship, Radiation Fetal Proteins Hydrogen-Ion Concentration Hypoglycemic Agents Insulin Intestinal Mucosa Intestine, Small Radiation, Ionizing Rats Reproducibility of Results Research Support, Non-U.S. Gov't Thymidine Tritium

Comprehensive evaluation and selection of the potential complex medium for industrial glutamate decarboxylase (GAD) production by Escherichia coli

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International Journal of Agricultural and Biological Engineering 2011年第2期4卷 74-82页

作者： Wanying Yao Xiao Wu Jun Zhu Bo Sun Department of Bioproducts and Biosystems Engineering University of Minnesota1390 Eckles AvenueSt.PaulMN 55108USA Department of Chemical Engineering Tshinghua UniversityBeijing 100084China Southern Research and Outreach Center University of MinnesotaWasecaMN 56093USA Northeastern Agricultural University Harbin 150030China

To address the need for producing large quantities of an enzyme,glutamate decarboxylase(GAD),this study evaluated the performance of the currently used complex and defined culture media in terms of biomass production,GAD yield,and byproducts accumulation,and selected the candidate(s)that could maximize the production of GAD by Escherichia coli(AS 1.505,the China General Microbiological culture Collection Center,Beijing,China).The highest yield of the enzyme per biomass in the final broth(4431 U/g)corresponded to the Luria Bertani medium(LBM),which was 1.16 times the second highest from the Terrific Broth medium(TBM),while the highest biomass production(4.5 g/L)was achieved in the cultivation with *** the byproducts,the profiles of all the media except the TK medium displayed a distinct exponential phase after six hour cultivation with a rapid byproducts generation,which could be attributed to the mixed acid fermentation caused by oxygen *** addition,the subsequent re-assimilation of acetate could reduce the loss of GAD accumulated by stabilizing the media *** comprehensive analysis showed that although the highest biomass production was achieved by TBM,the fastest intracellular accumulation of GAD occurred in the cultivation with LBM(184.64 U/(g·h)),which also was the cheapest medium(0.56$/103 U).Therefore,LBM was considered the potentially competitive candidate medium for large-scale production of GAD by ***.

关键词： culture media biomass yield GAD accumulation byproducts economic analysis Escherichia coli

Effect of the P1 Medium and the ECM Medium on Embryo Quality in IVF

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Journal of Reproduction and Contraception 2009年第2期20卷 67-72页

作者： Qian CHEN Ai-jun ZHANG Yun FENG Xiao-wei LU Dong-mei JI Zhi-peng XU Center of Reproductive Medicine Ruijing Hospital Medical School of Shanghai Jiaotong University Shanghai200025 China Center of Reproductive Medicine Drum Tower Hospital Medical School of Nanjin University Nanjin 210008 China

Objective To investigate the effect of the glucose-free preimplantation stage one （P1） medium and the ECM medium on embryo development quality in IVF. Methods The patients with ≥4 zygotes of 2PN were studied. A total of 201 retrieval cycles were included in a prospective randomized study. Each patient was herself control. Half of zygotes of 2PN were transferred into ECM medium （group A） and half into P1 medium （group B）for further culture. Embryo development was evaluated on the day of embryo transfer. The efficacy of ECM was compared with P1 as culture medium for the development of preimplantation embryos. Results No statistically significant differences were noted between the two groups regarding embryo-cleavage rate （97.13% vs 97.55%） and rate of normal-cleaving embryos （58.29% and 58.37%）. The rate of top-quality embryos was statistically higher in group A than in group B （27.59% vs 19.75%, P〈O.05）. Embryo quality, as assessed by morphological parameters （the amount of detached anuclear fragments 〉30%）, was better in group A than in group B （19.86% vs 21.75%）, however, there was no statistically significance. Both the rate of good-quality embryos （47.95% vs 46.17%） and available embryos （63.22% vs 61.19%） were higher in group A than in group B, but there was also no statistically significance. Conclusion The ECM medium may be associated with a better embryo quality compared with the P1 medium.

关键词： culture media IVF preimplantation embryo development

First Report on Rhizome Rot Disease of Curcuma longa Caused by Fusarium solani in Bangladesh

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American Journal of Plant Sciences 2022年第4期13卷 506-516页

作者： Md. Sabbir Ahmmed Fatema Akter Nisha Nuhu Alam Department of Botany Faculty of Biological Sciences Jahangirnagar University Dhaka Bangladesh

Turmeric (Curcuma longa L.) is a valuable medicinal plant as well as spice crop in Bangladesh. The rhizome rot disease is a severe danger to turmeric cultivation. The current study sought to identify the fungal pathogen linked to turmeric rhizome rot disease. Rhizome of turmeric with distinct rotted symptoms was collected from the experimental site of the Botanical Garden, Jahangirnagar University, Bangladesh. The sample was screened to isolate the causative fungal pathogen through the tissue planting technique. Macro and micro-morphological characterization based on colony appearance, mycelial and conidial characteristics primarily identified the fungus as Fusarium sp. The ITS sequence of rDNA of the fungus exhibited 99 to 100 percent similarity with the other F. solani species formerly deposited in the NCBI database which confirmed the fungal identity as F. solani. An in vitro pathogenicity test validated the pathogenic nature of the fungus. Growth behaviors of the fungus were evaluated on different solid culture media viz., Potato dextrose agar, Potato sucrose agar, Sabouraud dextrose agar and Hansen’s agar;temperature conditions (10?C, 15?C, 20?C, 25?C, 30?C and 35?C) and pH levels (pH 4, pH 5, pH 6, pH 7 and pH 8). Maximum mycelial growth was obtained on PSA medium at 30?C temperature and pH 7 conditions. Current findings also conclude that F. solani favors a wide range of temperature and pH levels. To the best of our search, the present investigation revealed the relationship of F. solani with the rhizome rot disease of turmeric for the first time in Bangladesh.

关键词： Turmeric Fungal Disease Molecular Identification culture media Temperature pH

Selective Lactococcus Enumeration in Raw Milk

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Food and Nutrition Sciences 2013年第9期4卷 49-58页

作者： Laetitia Gemelas Véronique Rigobello Mai Huong Ly-Chatain Yann Demarigny Isara-Lyon Unité BioDyMIALyonFrance

The Lactococcus diversity in cow and goat raw milk was investigated. To do so, a protocol had to be established for the specific enumeration of lactococci. Eight agar media and one control medium were analysed to compare their proficiency in evaluating the Lactococcus population in raw milk: M17 Nal, Elliker, modified Elliker, PCA + milk, modified KCA, modified Chalmers, Turner, FSDA. The M17 medium was used as reference. Eighteen pure strains were tested on these media for their selectivity towards lactococci: six Lactococcus species or subspecies, three Leuconostoc, three Enterococcus, two Lactobacillus, one Streptococcus thermophilus, one Pseudomonas fluorescens, one Escherichia coli and one Staphylococcus aureus. All these bacteria were chosen for their regular presence in raw milk. The KCA medium proved to be the most selective towards lactococci, on condition that 1) we discriminated the colonies using the catalase test and 2) we subtracted the Enterococcus population counted on BEA. However, it was not possible to separate the Streptococcus from the Lactococcus colonies on KCA. The “Lactococcus-like” population including these two genera was estimated at a mean level of 3.18 log(cfu)/mL and 4.14 log(cfu)/mL in cow and goat raw milk respectively. This is consistent with the data already published.

关键词： Lactococcus culture media Raw Milk LAB Modified KCA

Morphology and Molecular Identification of Dry Fish Fungus Cunninghamella blakesleeana from Small Indigenous Fish “Kachki” Corica soborna (Hamilton 1822) in Bangladesh

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American Journal of Plant Sciences 2023年第11期14卷 1316-1326页

作者： Abdullah Al Masud Ismot Ara Nuhu Alam Department of Zoology Jagannath University Dhaka Bangladesh Department of Zoology Jahangirnagar University Savar Bangladesh Department of Botany Jahangirnagar University Savar Bangladesh

The present experiment was conducted to investigate a dry fish fungus, Cunnighamella blakesleeana, which was identified from the infected part of the Corica soborna, locally named as Kachki fish. Mycelium was hyaline, often with granular content, and conidiophores were erected, with verticillate or solitary branches. Zygospores were globose, tuberculate, suspensors equal, smooth, hyaline and heterothallic. Using ITS4 and ITS5 primers, the 740 bp-long ITS region was amplified and sequenced. The ITS region sequences had reciprocal homologies of 98% to 100%. The findings showed that several species of C. blakesleeana fall into the same cluster. It has been determined by molecular data that the fungus we had studied was C. blakesleeana. The maximum mycelial growth (95.33 mm) was observed in the PDA medium, followed by the PSA medium, and the lowest growth (65.50 mm) was measured in the HPA medium in the study of the impact of culture media on the mycelial growth of C. blakesleeana. The influence of temperature on the radial mycelial growth of C. blakesleeana on PDA medium was investigated through five different temperatures. Although pH is a crucial factor in understanding the ecology of spoilage fungus, the highest mycelial growth of C. blakesleeana (88.25 mm) was seen at pH 7, followed by pH 8 and pH 6, while pH 9 was revealed to have the lowest mycelial growth. The outcome suggested that C. blakesleeana thrived in neutral environments.

关键词： culture media Molecular Identification Temperature pH Vegetative Growth

The in vitro myelin formation in neurospheres of human neural stem cells

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Chinese Journal of Traumatology 2003年第6期6卷 341-345页

作者：杨立业郑佳坤刘相名惠国桢郭礼和 Department of Neurosurgery Chaozhou Central Hospital Guangdong Province Department of Neurosurgery The First Hospital of Soochow University Institute of Biochemistry and Cell Biology Shanghai Institute for Biological Sciences Chinese Academy of Sciences

Objective: To explore the culture conditions of human neural stem cells and to investigate the ultrastructure of neurospheres. Methods: The cells from the embryonic human cortices were mechanically dissociated. N2 medium was adapted to culture and expand the cells. The cells were identified by immunocytochemistry and EM was applied to examine the ultrastructure of neurospheres. Results: The neural stem cells from human embryonic brains were successfully cultured and formed typical neurospheres in suspension, and most of the cells expressed vimentin, which was a marker for neural progenitor cells, and the cells could differentiate into neurons, astrocytes and oligodendrocytes. In vitro myelin formation in neurospheres were observed at an early stage of culture. Conclusions: Human neural stem cells can be cultured from embryonic brains, can form the typical neurospheres in suspension in vitro and have the ability of myelinating, and may be potential source for transplantation in treating myelin disorders.

关键词： Transplantation Stem cells culture media Myelin