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A new glimpse of FadR-DNA crosstalk revealed by deep dissection of the E. coil FadR regulatory protein

Protein & Cell 2014年第12期5卷 928-939页

作者： Yongchang Zhang College of Life Sciences Nanjing Normal University Nanjing 210023 China"2 State Key Laboratory for Diagnosis and Treatment of Infectious Diseases Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases First Affiliated Hospital Zhejiang University School of Medicine Hangzhou 310003 China Department of Medical Microbiology and Parasitology Center for Infection and Immunity Zhejiang University School of Medicine Hangzhou 310058 China College of Life Science and Technology Guangxi University Nanning 530004 China

Escherichia coil （E. coli） FadR regulator plays dual roles in fatty acid metabolism, which not only represses the fatty acid degradation （fad） system, but also activates the unsaturated fatty acid synthesis pathway. Earlier structural and biochemical studies of FadR protein have provided insights into interplay between FadR protein with its DNA target and/or ligand, while the missing knowledge gap （esp. residues with indirect roles in DNA binding） remains unclear. Here we report this case through deep mapping of old E. coil fadR mutants accumulated. Molecular dissection of E. coil K113 strain, a fadR mutant that can grow on decanoic acid （C10） as sole carbon sources unexpectedly revealed a single point mutation of T178G in fadR locus （W60G in FadRk113）. We also observed that a single genetically- recessive mutation of W60G in FadR regulatory protein can lead to loss of its DNA-binding activity, and thereby impair all the regulatory roles in fatty acid metabolisms. Structural analyses of FadR protein indicated that the hydrophobic interaction amongst the three amino acids （W60, F74 and W75） is critical for its DNA-binding ability by maintaining the configuration of its neighboring two β-sheets. Further site-directed mutagenesis analyses demonstrated that the FadR mutants （F74G and/orW75G） do not exhibit the detected DNA-binding activity, validating above structural reasoning.

关键词： FadR fatty acid metabolism crosstalk

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Harnessing self-heating effect for ultralow-crosstalk electro-optic Mach–Zehnder switches

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Photonics Research 2023年第10期11卷 1757-1769页

作者： PENG BAO QIXIANG CHENG JINLONG WEI GIUSEPPE TALLI MAXIM KUSCHNEROV RICHARD V.PENTY Centre for Photonic Systems Electrical Engineering DivisionDepartment of EngineeringUniversity of CambridgeCambridge CB30FAUK Huawei Technologies Duesseldolf GmbH European Research Center80992 MunichGemany Current address:Peng Cheng Laboratory Shenzhen 518000China

This paper presents a novel approach to counterbalance free-carrier-absorption(FCA) in electro-optic(E-O)Mach–Zehnder interferometer(MZI) cells by harnessing the self-heating effect. We show insights on crosstalk limitations in MZIs with direct carrier-injection and provide a detailed design methodology on a differential phase shifter pair. Leveraging both free-carrier dispersion(FCD) and self-heating effects, our design enables arbitrary phase tuning with balanced FCA loss in the pair of arms, eliminating the need for additional phase corrections and creating ultralow crosstalk MZI elements. This neat design disengages from the commonly used nested structure, thus providing an opportunity of embedding tunable couplers for correcting imperfect splitting ratios given that only two are needed. We show that with the use of tunable directional couplers, a standard±10 nm process variation is tolerated, while achieving a crosstalk ratio below-40 dB. By direct carrier injection in both arms, the proposed device operates at nanosecond scales and can bring about a breakthrough in the scalability of E-O switch fabrics, as well as other silicon integrated circuits that have stringent requirements for crosstalk leakage.

关键词： crosstalk interferometer breakthrough

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Cellular crosstalk during cholestatic liver injury

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Liver Research 2017年第1期1卷 26-33页

作者： Joanne Thomson Laura Hargrove Lindsey Kennedy Jennifer Demieville Heather Francis Research Central Texas Veterans Healthcare SystemTXUSA Digestive Disease Research Center Baylor Scott&White HealthTXUSA Medicine Texas A&M Health Science CenterTempleTXUSA

The functions of the liver are very diverse.From detoxifying blood to storing glucose in the form of glycogen and producing bile to facilitate fat digestion,the liver is a very active and important organ.The liver is comprised of many varied cell types whose functions are equally diverse.Cholangiocytes line the biliary tree and aid in transporting and adjusting the composition of bile as it travels to the gallbladder.Hepatic stellate cells and portal fibroblasts are located in different areas within the liver architecture,but both contribute to the development of fibrosis upon activation after liver injury.Vascular cells,including those that constitute the peribiliary vascular plexus,are involved in functions other than blood delivery to and from the liver,such as supporting the growth of the biliary tree during development.Mast cells are normally found in healthy livers but in very low numbers.However,after injury,mast cell numbers greatly increase as they infiltrate and release factors that exacerbate the fibrotic response.While not an all-inclusive list,these cells have individual roles within the liver,but they are also able to communicate with each other by cellular crosstalk.In this review,we examine some of these pathways that can lead to an increase in the homeostatic dysfunction seen in liver injury.

关键词： Mast cells Paracrine Cholangiocytes Hepatic stellate cells crosstalk

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Recent Advances in Dissecting Stress-Regulatory crosstalk in Rice

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Molecular Plant 2013年第2期6卷 250-260页

作者： Rita Sharma Department of Plant Pathology and Genome Center University of California Davis CA 95616 USA Joint BioEnergy Institute Emeryville CA 94710 USA Laboratory of Phytopathology Faculty of Bioscience Engineering Ghent University B-9000 Ghent Belgium Department of Plant Molecular Systems Biotechnology and Crop Biotech Institute Kyung Hee University Yongin 446-701 Korea

Biotic and abiotic stresses impose a serious limitation on crop productivity worldwide. Prior or simultaneous exposure to one type of stress often affects the plant response to other stresses, indicating extensive overlap and cross-talk between stress-response signaling pathways. Systems biology approaches that integrate large genomic and prot-eomic data sets have facilitated identification of candidate genes that govern this stress-regulatory crosstalk. Recently, we constructed a yeast two-hybrid map around three rice proteins that control the response to biotic and abiotic stresses, namely the immune receptor XA21, which confers resistance to the Gram-negative bacterium, Xanthomonas oryzae pv. oryzae; NH1, the rice ortholog of NPR1, a key regulator of systemic acquired resistance; and the ethylene-responsive transcription factor, SUBIA, which confers tolerance to submergence stress. These studies coupled with transcriptional profiling and co-expression analyses identified a suite of proteins that are positioned at the interface of biotic and abiotic stress responses, including mitogen-activated protein kinase 5 （OsMPK5）, wall-associated kinase 25 （WAK25）, sucrose non-fermenting-l-related protein kinase-1 （SnRK1）, SUBIA binding protein 23 （SAB23）, and several WRKY family tran- scription factors. Emerging evidence suggests that these genes orchestrate crosstalk between biotic and abiotic stresses through a variety of mechanisms, including regulation of cellular energy homeostasis and modification of synergistic and/or antagonistic interactions between the stress hormones salicylic acid, ethylene, jasmonic acid, and abscisic acid.

关键词： abiotic biotic crosstalk defense response hormone systems biology stress.

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Theoretical Analysis on Inter-Core crosstalk Suppression Model for Multi-Core Fiber

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China Communications 2016年第8期13卷 192-197页

作者： Jiajing Tu Xueqin Xie Keping Long School of Computer and Communication Engineering University of Science and Technology Beijing (USTB)

Decreasing mode coupling coefficient(κ) is an effective approach to suppress the inter-core crosstalk. Therefore, we deploy a low index rod and rectangle trench in the middle of two neighboring cores to reduce κ so that the overlap of electric field distribution can be suppressed. We also propose approximate analytical solution(AAS) for κ of two crosstalk suppression models, which are two cores with one low index rod deployed in the middle and two cores with one low index rectangle trench deployed in the middle. We then do some modification for the results obtained by AAS and the modified results are proved to agree well with that obtained by finite element method(FEM). Therefore, we can use the modified AAS to get inter-core crosstalk for abovementioned two models quickly.

关键词： multi-core fiber crosstalk mode coupling coefficient

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crosstalk between mesenchymal stem cell and cardiomyocytes prevents pathological myocardial hypertrophy

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中国药理学通报 2015年第B11期31卷 150-151页

作者： CAIBen-zhi TANXue-ying ZHANGYong LIxing-da WANGXin-yue QANGYang CardiocascularResearchInstituteHarbinMedicalUniversityHarbin

Aim The present study aims to investigate whether BMSCs transplantation may inhibit hypertrophic hearts and its underlying mechanisms. Background There is no evidence so far that Bone marrow-derived mesenchy- mal stem cells （BMSCs） can heal pathological myocardial hypertrophy. Methods To observe the antihypertrophic actions, BMSCs was indirectly cocultured with NRVCs in vitro, or intramyocardially transplanted into hypertrophic hearts in vivo. Results ISO-induced typical hypertrophic characteristics of cardiomyocytes were obviously preven- ted by BMSCs in the co-culture model in vitro and after BMSCs transplantation in vivo. Furthermore, the activation of the Ca2＋/calcineurin/NFATc3 hypertrophic pathway was shown abrogated in the presence of BMSCs both in vitro and in vivo. Interestingly, blockage of VEGF release from BMSCs but not bFGF and IGF-1 can abolish the protec- tive effects of BMSCs on cardiomyocytes hypertrophy. Consistently, VEGF administration attenuated ISO-induced BNP and β-MHC expression and the activation of Ca2＋/cal- the enlargement of cellular size, the augment of ANP, cineurin/NFATc3 hypertrophic pathway, and these can be abrogated by blocking VEGFR-1, indicating VEGFR-1 is involved in the antihypertrophic role of VEGF. We further find that the ample VEGF secretion contributing to the anti-hypertrophic effects of BMSCs originates from BMSCs interplay with cardiac cells but not BMSCs or cardiomyo- cytes alone. Conclusions Thus, mesenchymal stem cells are able to inhibit myocardial hypertrophy via interacting with cardiomyocytes so as to promote VEGF release which inhibits the activation of the Ca2＋/calcineurin/NFATc3 hypertrophic signaling pathway in cardiac cells, in addition to its well-recognized ability to ameliorate myocardial injuries by replacing dead cells.

关键词： mesenchymal stem cell cardiomyocyte crosstalk hypertrophy remodeling

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Gut-lung crosstalk in pulmonary involvement with inflammatory bowel diseases

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World Journal of Gastroenterology 2013年第40期19卷 6794-6804页

作者： Hui Wang Jing-Shi Liu Shao-Hua Peng Xi-Yun Deng De-Mao Zhu Sara Javidiparsijani Gui-Rong Wang Dai-Qiang Li Long-Xuan Li Yi-Chun Wang Jun-Ming Luo Department of Radiation Oncology the Affiliated Hunan Provincial Tumor HospitalXiangya Medical SchoolCentral South University Department of Anesthesiology the Affiliated Hunan Provincial Tumor Hospital at Central South University Department of Pathology Medical College of Hunan Normal University Department of Pathology Changsha Central Hospital Department of Surgery SUNY Upstate Medical University Department of Pathology the Second Affiliated HospitalXiangya Medical SchoolCentral South University Department of Neurology Guangdong Medical College Affiliated Hospital Department of Pathology Qinghai Provincial People's Hospital

Pulmonary abnormalities,dysfunction or hyper-reactivity occurs in association with inflammatory bowel disease(IBD) more frequently than previously recognized.Emerging evidence suggests that subtle inflammation exists in the airways among IBD patients even in the absence of any bronchopulmonary symptoms,and with normal pulmonary functions. The pulmonary impairment is more pronounced in IBD patients with active disease than in those in remission. A growing number of case reports show that the IBD patients develop rapidly progressive respiratory symptoms after colectomy,with failure to isolate bacterial pathogens on repeated sputum culture,and often request oral corticosteroid therapy. All the above evidence indicates that the inflammatory changes in both the intestine and lung during IBD. Clinical or subclinical pulmonary inflammation accompanies the main inflammation of the bowel.Although there are clinical and epidemiological reports of chronic inflammation of the pulmonary and intestinal mucosa in IBD,the detailed mechanisms of pulmonaryintestinal crosstalk remain unknown. The lung has no anatomical connection with the main inflammatory site of the bowel. Why does the inflammatory process shift from the gastrointestinal tract to the airways? The clinical and subclinical pulmonary abnormalities,dysfunction,or hyper-reactivity among IBD patients need further evaluation. Here,we give an overview of the concordance between chronic inflammatory reactions in the airways and the gastrointestinal tract. A better understanding of the possible mechanism of the crosstalk among the distant organs will be beneficial in identifying therapeutic strategies for mucosal inflammatory diseases such as IBD and allergy.

关键词： Inflammatory bowel disease Pulmonary symptoms Gut-lung crosstalk Biao-Li relationship Social manner

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胶质瘤细胞与血管内皮细胞的信号crosstalk对肿瘤细胞增殖和侵袭的影响

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山东大学学报（医学版） 2021年第2期59卷 1-6,13页

作者：顾金海路宁顾珈榕文玉军强媛媛和祯泉杨勇王峰孙涛牛建国宁夏医科大学宁夏颅脑疾病重点实验室宁夏银川750004 银川市第一人民医院神经外科宁夏银川750001 宁波大学医学院预防医学学科系浙江宁波315211

目的探讨胶质瘤细胞与血管内皮细胞之间的信号crosstalk及其对肿瘤细胞增殖和侵袭的影响。方法将人脑微血管内皮细胞(HBMEC)分4组培养:对照组为HBMEC常规培养,血管内皮生长因子(VEGF)组为HBMEC在含VEGF_(165)(50 ng/mL)的内皮细胞培养基... 详细信息

目的探讨胶质瘤细胞与血管内皮细胞之间的信号crosstalk及其对肿瘤细胞增殖和侵袭的影响。方法将人脑微血管内皮细胞(HBMEC)分4组培养:对照组为HBMEC常规培养,血管内皮生长因子(VEGF)组为HBMEC在含VEGF_(165)(50 ng/mL)的内皮细胞培养基(ECM)中培养,U87MG共培养组为HBMEC与胶质瘤细胞U87MG共培养,U251共培养组为HBMEC与胶质瘤细胞U251共培养,然后采用ELISA检测胶质瘤细胞及组织因子VEGF对血管内皮细胞分泌CXCL8的影响。将人脑胶质瘤细胞U87MG或U251分4组培养:对照组为U87MG或U251常规培养,U87MG/U251+HBMEC组为U87MG或U251与HBMEC共培养,U87MG/U251+HBMEC+rhCXCL8组为U87MG或U251与HBMEC在含Akt通路的激动剂rhCXCL8(50 ng/mL)的DMEM中共培养,U87MG/U251+HBMEC+LY294002组为U87MG或U251与HBMEC在含Akt通路的抑制剂LY294002(1μmol/L)的DMEM中共培养,分别采用Western blotting、CCK-8、Transwell侵袭实验检测血管内皮细胞及其分泌CXCL8对胶质瘤细胞的增殖、侵袭以及Akt蛋白表达的影响。结果与对照组比较,VEGF组、U87MG共培养组、U251共培养组中HBMEC培养体系的CXCL8分泌量升高(Pcrosstalk机制,在胶质瘤增殖和侵袭中发挥重要作用。

关键词：胶质瘤血管内皮细胞 crosstalk 血管内皮生长因子 CXCL8 Akt

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Auxin-Oxylipin crosstalk:Relationship of Antagonists

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Journal of Integrative Plant Biology 2011年第6期53卷 429-445页

作者： Maik Hoffmann Mathias Hentrich Stephan Pollmann Centro de Biotecnología y Genómica de Plantas(U.P.M.-I.N.I.A.) Parque Científico y Tecnológico de la U.P.M. Campus de Montegancedo Ruhr-Universitt Bochum Lehrstuhl fr PflanzenphysiologieUniversittsstrasse

Phytohormones regulate a wide array of developmental processes throughout the life cycle of plants. Herein, the various plant hormones may interact additively, synergistically, or antagonistically. By their cooperation they create a delicate regulatory network whose net output largely depends on the action of specific phytohormone combinations rather than on the independent activities of separate hormones. While most classical studies of plant hormonal control have focused mainly on the action of single hormones or on the synergistic interaction of hormones in regulating various developmental processes, recent work is beginning to shed light on the crosstalk of nominally antagonistic plant hormones, such as gibberellins and auxins with oxylipins or abscisic acid. In this review, we summarize our current understanding of how two of the first sight antagonistic plant hormones, i.e. auxins and oxylipins, interact in controlling plant responses and development.

关键词： Auxin biosynthesis crosstalk hormone jasmonic acid.

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信息交互作用(crosstalk)

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中华预防医学杂志 2003年第5期37卷 72-72页

作者：方福德中国医学科学院基础医学研究所 100005北京

泛指信息之间的相互影响、相互协调和相互作用，包括细胞之间的信息通讯、信息物质之间的作用和信息转导途径或通道之间的作用。因此，信息交谈是一个多层次和具有时空特征的信息交流、协调和作用的网络。细胞时刻处于信息之中，必须有... 详细信息

泛指信息之间的相互影响、相互协调和相互作用，包括细胞之间的信息通讯、信息物质之间的作用和信息转导途径或通道之间的作用。因此，信息交谈是一个多层次和具有时空特征的信息交流、协调和作用的网络。细胞时刻处于信息之中，必须有正确的信息交谈才能保持正常的功能。

关键词： crosstalk 细胞

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