检索结果-南通市图书馆

binding of Mono, Bi and Tridomain Proteins to Zwitterionic and Anionic Vesicles： Asymmetric Location of Anionic Phospholipids in Mixed Zwitterionic/Anionic Vesicles and cooperative binding

引用

Journal of Life Sciences 2011年第3期5卷 167-181页

作者： Francisco Torrens Gloria Castellano Institute for Molecular Science University of Valencia Valencia E-46071 Spain Energesis Chair of Interdisciplinary Technology Saint Vincent Martyr Catholic University of Valencia Valencia E-46003 Spain

Lysozyme, myoglobin and BSA were used as models of globular proteins covering a wide range of pl. The purpose is to extend the studies to anionic lipid bilayers. Electrostatics is studied in cationic protein adsorption to zwitterionic PC and anionic mixed PC/PG SUVs. Protein adsorption is investigated in SUVs along with changes of fluorescence emission spectra. Partition coefficients and cooperativity parameters are calculated. At pl binding obtains maximum while at lower or higher pHs binding decreases. In Gouy-Chapman formalism activity coefficient goes with square charge, which deviations indicate asymmetric location of anionic phospholipid in the inner leaflet, in mixed SUVs for lysozyme- and myoglobin-PC/PG systems, in agreement with experiments and molecular dynamics simulations. Vesicles bind myoglobin anti-cooperatively while lysozyme-BSA cooperativitivey. A model is proposed for both, which composes two protein sub-layers with different structures and properties. Hill coefficient reflects subunit cooperativity of bi and tridomain proteins.

关键词： Protein-lipid interaction sigmoid adsorption isotherm transbilayer asymmetry anionic phospholipid asymmetric location cooperative binding hill plot.

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Synthesis of a Novel Aromatic Diamino-bridged Bis(β-cyclodextrin) and its Inclusion Complexation with Dyes Molecules

引用

Chinese Chemical Letters 2006年第12期17卷 1543-1546页

作者： Yah ZHAO Xiao Qing LIU Hong You ZHU Zi Ming YANG Rong HUANG College of Chemistry and Chemical Engineering Yunnan Normal University Kunming 650092 School of Chemical Science and Technology Yunnan University Kunming 650091 Experimental Center Yunnan University Kunming 650091

A novel brideed bis（β-cvclodextrin）, 4, 4＇-diaminodiphenyl ether-bfiged-bis （6-aimino-6-deoxv-β-cyclodextrin） 3, has been synthesized and its inclusion complexation behavior with three linear＇guest dyes （AR, NR and MB ） has been investigated by. means of fluorescence spectrometry. The-result obtained demonstrated that the novel bridged bis（β-cyclodextnn） showed much higher affinities towards guest dyes than native β-cyclodextrin.

关键词： Bridged bis(β-cyclodextrin)s cooperative binding inclusion complexation dye.

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：