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Microengineered platforms for characterizing the contractile function of in vitro cardiac models

Microsystems & Nanoengineering 2022年第1期8卷 29-50页

作者： Wenkun Dou Manpreet Malhi Qili Zhao Li Wang Zongjie Huang Junhui Law Na Liu Craig A.Simmons Jason T.Maynes Yu Sun Department of Mechanical and Industrial Engineering University of TorontoTorontoON M5S 3G8Canada Program in Molecular Medicine The Hospital for Sick ChildrenTorontoON M5G 1X8Canada Department of Biochemistry University of TorontoTorontoON M5S 1A8Canada Institute of Robotics and Automatic Information System and the Tianjin Key Laboratory of Intelligent Robotics Nankai UniversityTianjin 300350China School of Mechanical&Automotive Engineering Qilu University of Technology(Shandong Academy of Sciences)Jinan 250353China School of Mechatronics Engineering and Automation Shanghai UniversityShanghai 200444China Institute of Biomedical Engineering University of TorontoTorontoON M5S 3G9Canada Translational Biology&Engineering Program Ted Rogers Centre for Heart ResearchTorontoON M5G 1M1Canada Department of Anesthesiology and Pain Medicine University of TorontoTorontoON M5S 1A8Canada Department of Anesthesia and Pain Medicine The Hospital for Sick ChildrenTorontoON M5G 1X8Canada Department of Electrical and Computer Engineering University of TorontoTorontoON M5S 3G4Canada Department of Computer Science University of TorontoTorontoON M5T 3A1Canada

Emerging heart-on-a-chip platforms are promising approaches to establish cardiac cell/tissue models in vitro for research on cardiac physiology,disease modeling and drug cardiotoxicity as well as for therapeutic *** still exist in obtaining the complete capability of in situ sensing to fully evaluate the complex functional properties of cardiac cell/tissue *** to contractile strength(contractility)and beating regularity(rhythm)are particularly important to generate accurate,predictive *** new platforms and technologies to assess the contractile functions of in vitro cardiac models is essential to provide information on cell/tissue physiologies,drug-induced inotropic responses,and the mechanisms of cardiac *** this review,we discuss recent advances in biosensing platforms for the measurement of contractile functions of in vitro cardiac models,including single cardiomyocytes,2D monolayers of cardiomyocytes,and 3D cardiac *** characteristics and performance of current platforms are reviewed in terms of sensing principles,measured parameters,performance,cell sources,cell/tissue model configurations,advantages,and *** addition,we highlight applications of these platforms and relevant discoveries in fundamental investigations,drug testing,and disease ***,challenges and future outlooks of heart-on-a-chip platforms for in vitro measurement of cardiac functional properties are discussed.

关键词： cardiac contractile regularity

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Morphology of cylindrical cell sheets with embedded contractile ring

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Applied Mathematics and Mechanics(English Edition) 2019年第12期40卷 1847-1860页

作者： Nan NAN Guohui HU Shanghai Key Laboratory of Mechanics in Energy Engineering Shanghai Institute of Applied Mathematics and MechanicsSchool of Mechanics and Engineering ScienceShanghai University

The behavior of large deformations of cellular tissues is usually affected by the local properties of cells and their interactions,resulting in folding which acts as an important role in the embryonic development,as well as growing and spreading of a tumor,which can rapidly promote the stereo complexity of the architecture of the *** the present study,a cylindrical vertex model is constructed to explore the morphology of the tubular cell sheets subject to an embedded contractile *** is found that an inner region of the contractile ring in equilibrium will protrude from the tube wall,and it will suddenly collapse when the contractile strength exceeds a threshold,indicating the occurrence of a *** results on the effect of embedded contraction in the tubular shell are quite different from the planar cases,which can reveal the importance of the interaction between the geometric and material non-linearity in cylindrical *** dependence of the large deformation on the bending modulus parameters and contraction strength is also analyzed for the cylindrical cell shell.

关键词： vertex model cylindrical cell sheet contractile ring large deformation protrusion

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Remodeled structure and reduced contractile responsiveness of ocular ciliary artery in spontaneously hypertensive rats

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International Journal of Ophthalmology(English edition) 2019年第3期12卷 363-368页

作者： Ya-Ru Dong Claire E Gustafson Jun Wang Ji-Zhe Cui Takeshi Yoshitomi Department of Ophthalmology the Second Hospital of Jilin University Department of Ophthalmology Akita University School of Medicine Division of Immunology and Rheumatology Department of Medicine Stanford University

AIM: To investigate the alterations in both structure and contractile responsiveness of ocular ciliary artery(OCA) in spontaneously hypertensive rat(SHR).METHODS: In this experiment, 20-week-old male SHR and Wistar Kyoto rat(WKY) were studied. The heart rate(HR), the blood pressure(BP; the systolic BP and the diastolic BP) of rats with an electronic sphygmomanometer were measured. Vascular morphometry and isometric tension measurement were used to investigate the alterations in structure and contractility of ***: A general narrowing of OCAs was observed in SHR compared to the control WYK. In SHR, the media of OCAs were thicker, the luminal diameters were smaller, and the media-to-lumen ratios were higher when compared with WKY(P0.05). Indomethacin(10 μmol/L) decreased the contractions induced by norepinephrine in OCAs from WKY(P0.05).CONCLUSION: Our results demonstrate that the structure and function of OCAs are altered in hypertension. OCAs from SHR are remodeled with decreased lumen diameter and increased media-to-lumen ratio. Moreover, the contractile responsiveness of OCAs from SHR is diminished due to the disruption of vasoconstrictive effect of prostaglandins.

关键词： remodeled structure contractile responsiveness ocular ciliary artery hypertension spontaneously hypertensive rat

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Reduced bile duct contractile function in rats with chronic hyperglycemia

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Health 2010年第9期2卷 1072-1077页

作者： Chi-Ming Liu Hui-Chen Su Yen-Ting Wang Tao-Hsin Tung Pesus Chou Yiing-Jeng Chou Jorn-Hon Liu Jan-Kan Chen Cheng-Hsin General Hospital Chi-Mei Medical Center

The incidence of gallstone is higher in patients with diabetes mellitus than in general popula- tion. It is generally attributed to hypomotility and lowered emptying function of the gallblad- der. In this study, we investigate if chronic hy- perglycemia is correlated with reduced contrac- tile function of the bile ducts in rat. Hypergly- cemic rats were induced by streptozotocin-nic-otinamide treatment. Hyperglycemic rats were sacrificed eight months after induction and bile ducts were removed for the subsequent studies. The bile duct contractility of the normal rats is consistently higher than that of the hypergly- cemic rats. The contractities were measured to be 5.5 ± 0.2 mg vs. 4.2 ± 0.1 mg without CCK stimulation, and 5.5 ± 0.3 mg vs. 7.9 ± 0.4 mg with CCK stimulation, respectively for hypergly-cemic and normal rats. There was no significant difference in plasma CCK concentration in hy- perglycemic rats and normal rats. The expres- sion of CCK-A receptor protein in the bile duct tissue was decreased in hyperglycemic rats compared with that of the normal rats, and it may, at least in part, responsible for a reduced contractility. A reduced bile duct motility may cause bile retention, and may be one of the factors predispose to gallstone formation in type 2 diabetes patients, which is characterized with chronic hyperglycemia.

关键词： Hyperglycemia Bile Duct contractile

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HEART FAILURE MYOCYTE contractile MECHANICS AND ITS contractile PROTEIN GENE EXPRESSION

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Chinese Journal of Biomedical Engineering(English Edition) 1998年第2期7卷 54-61页

作者： Wang Zhengrong Wang Ling Cai Shaohui Yin Huahu(Biomedical Engineering Dept,West China University ofMedical Sciences, Chengdu,Sichuan 610041, China)

It is not certain whether the myocyte contractile function in the heartfailure is normal according to recent some studies,therefore the contractile functionof the myocytes with the heart failure should be studied. The myocyte heart failuremodel was set up by the method of stretching the cultured myocytes,and the myocytes with and without the heart failure were measured the contractile mechanicscharacteristic,the parameters being the maximal contractive velocity of the myocyte(Vmax), the maximal shortening length of myocyte contraction (ΔLmax) and themaximal decrease of the myocyte size during contraction (ΔSmax), by the videoedgedetector system. And then the protein genes (including α-MHC and β-MHC)expression of these myocytes were quantitatively measured by the dot blotting. Thecorrelative and regressive analysis were used to analyze the relationship between themyocyte contractile mechanics characteristic and the contractile protein genes expression level. The results showed that the contractile mechanics parameters,Vmax=41．5±6．0(μm/sec), △Lmax= 10．6±1．9 (% ) and △Smax= 14．3± 2．3 (% ), in themyocytes with the heart failure are statistically significant lower than the normalmyocytes (Pcontractile protein gene (αMHC) expression level in the heart failure myocytes is statistically significant lowerthan that in the normal myocytes,but β-MHC gene expression level is statisticallysignificant higher than the normal myocytes. The α-MllC gene expression level is apositive correlation coefficient with the myocyte contractile mechanics parametersseparately,covering Vmax,△Lmax and △max; but the β- MHC gene expression levelis a negative correlation coefficietn with the myocyte contractile mechanics parameters separately.

关键词： myocyte contractile cell mechanics gene expression heart failure contractile protein

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Etiology of Skeletal Muscle Injuries

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北京体育大学学报 1995年第S2期18卷 24-33页

作者： LU Dinghou (64 years old, professor)

Based on the results of our observation, the delayed alteration of contractile structures resulted from the delayed predominant degradation of contractile proteins of skeletal muscle constitute the background of the chronic or acute skeletal muscle injury. There fore,the arrangement of workload should be considered as the most important factor in preventing skeletal muscle injury.

关键词： contractile protein Predominant degradation contractile structure Delayed alteration Muscle injury

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MAPKs represent novel therapeutic targets for gastrointestinal motility disorders

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World Journal of Gastrointestinal Pathophysiology 2011年第2期2卷 19-25页

作者： Eikichi Ihara Hirotada Akiho Kazuhiko Nakamura Sara R Turner Justin A MacDonald Department of Medicine and Bioregulatory Science Graduate School of Medical SciencesKyushu University3-1-1 MaidashiHigashikuFukuoka 812-8582Japan Smooth Muscle Research Group Department of Biochemistry & Molecular Biology University of CalgaryFaculty of Medicine

The number of patients suffering from symptoms associated with gastrointestinal(GI) motility disorders is on the rise. GI motility disorders are accompanied by alteration of gastrointestinal smooth muscle functions. Currently available drugs,which can directly affect gastrointestinal smooth muscle and restore altered smooth muscle contractility to normal,are not satisfactory for treating patients with GI motility disorders. We have recently shown that ERK1/2 and p38MAPK signaling pathways play an important role in the contractile response not only of normal intestinal smooth muscle but also of inflamed intestinal smooth muscle. Here we discuss the possibility that ERK1/2 and p38MAPK signaling pathways represent ideal targets for generation of novel therapeutics for patients with GI motility disorders.

关键词： Mitogen-activated protein kinase P38MAPK ERK1/2 Smooth muscle contractile dysfunction

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Electrophysiological and Inotropic Effects of Iodium-Heterocyclic Compound on lsolated Guinea Pig Papillary Muscles

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Journal of Medical Colleges of PLA(China) 1989年第2期4卷 120-123页

作者：曹维赵德化盛宝恒 Department of Pharmacology Fourth Military Medical UniversityXi'an

The effects of iodium-heterocyclic compound（3,6-di-[dimethytamino]-dibcnzopyrodoniun formate,IHC-64）on the action potentials and contractile force of the isolatedguinca pig papillary muscles were investigatad.50 μmol/L IHC-64 only inhibited contractile forceand had no effect on APA,dv/dt max,APD50 and APD90 of fast action potentials in Tyrode’s *** of these parametres were depressed by further increasing concentration of ***-rich Tyrodc’s solution,the depressive action of IHC-64 on slow action potentials andcontractile force could be antagonized by incrcased extracellular *** results suggesr thatIHC-64 inhibit not only slow calcium influx but also fast sodium influx,and it may.（?）new calci-urn channd blockcr.

关键词： iodium-heterocyclic compound calcium channel blocker papillary muscle action polential contractile force of myocardium

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