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Three-dimensional reconstruction of light microscopy image sections： present and future

Frontiers of Medicine 2015年第1期9卷 30-45页

作者： Yuzhen Wang Rui Xu Gaoxing Luo Jun Wu Institute of Burn Research Southwest Hospital State Key Laboratory of Trauma Burns and Combined lnjury Chongqing Key Laboratory for Diseases Proteomics the Third Military Medical University Chongqing 400038 China

Three-dimensional （3D） image reconstruction technologies can reveal previously hidden microstruc-tures in human tissue. However, the lack of ideal, non-destructive cross-sectional imaging techniques is still a problem. Despite some drawbacks, histological sectioning remains one of the most powerful methods for accurate high-resolution representation of tissue structures. computer technologies can produce 3D representations of interesting human tissue and organs that have been serial-sectioned, dyed or stained, imaged, and segmented for 3D visualization. 3D reconstruction also has great potential in the fields of tissue engineering and 3D printing. This article outlines the most common methods for 3D tissue section reconstruction. We describe the most important academic concepts in this field, and provide critical explanations and comparisons. We also note key steps in the reconstruction procedures, and highlight recent progress in the development of new reconstruction methods.

关键词： microtomy 3D imaging computer-assisted image processing 3D printing tissue scaffold

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Research on focal nodular hyperplasia with MSCT and postprocessing

Research on focal nodular hyperplasia with MSCT and postproc...

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第九届中国肿瘤微创治疗学术大会

作者： Yuan-Jian Liu Wei-Jun Fan Zhi-Dong Yuan Peng-Cheng Liu Chun-Rong Wang Wei-Qiang Yan Su-Mei Wang Jun-Hui Chen Zheng Liu Imaging Center Peking University Shenzhen Hospital State Key Laboratory of Oncology in Southern China Imaging and Interventional CenterCancer CenterSun Yat-sen University

＜正＞AIM:To investigate and evaluate the pathological features and diagnostic value of focal nodular hyperplasia （FNH） with multi-section spiral computed tomography （MSCT） and postprocessing. METHODS:A total of 25 patients with FNH who had undergone MSCT and postprocessing were included in the *** patients had been pathologically or clinically confirmed with FNH.A number of 75 cases of hepatic carcinomas,hemangiomas and adenomas were randomly selected at a same period for a comparative study. RESULTS:There was a single focus in 22 cases and multiple foci in 3 *** the plain scan,17 lesions showed hypodensity,7 isodensity and 4 hyperdensity （the case with fatty liver）.With contrast,28 lesions were enhanced evenly or in the nodules in the arterial phase;13 lesions still showed hyperdensity,11 lesions isodensity and 4 lesions hypodensity in the parenchymatous phase;in the delayed phase only 5 lesions showed hyperdensity but 9 lesions showed isodensity or slight hypodensity and 14 lesions showed *** lesions of 28 had central asteroid *** feeding arteries in postprocessing were seen in 24 lesions,and were integrated into the parenchymatous lesions with a gradual and smooth *** the contrary,there were no artery penetrated into the lesion found in any of comparative hepatic tumors. CONCLUSION:Doctors could make a correct diagnosis and differentiation of FNH on evaluation of the characteristic appearance on MSCT with postprocessing.

关键词： Angiography computer-assisted image processing Focal nodular hyperplasia Liver diseases X-ray Computed tomography

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