Three-dimensional reconstruction of light microscopy image sections： present and future

作     者：Yuzhen Wang Rui Xu Gaoxing Luo Jun Wu 

作者机构：Institute of Burn Research Southwest Hospital State Key Laboratory of Trauma Burns and Combined lnjury Chongqing Key Laboratory for Diseases Proteomics the Third Military Medical University Chongqing 400038 China 

出 版 物：《Frontiers of Medicine》 (医学前沿（英文版）)

年 卷 期：2015年第9卷第1期

页      面：30-45页

核心收录：

学科分类：0710[理学-生物学] 1002[医学-临床医学] 07[理学] 08[工学] 080203[工学-机械设计及理论] 0802[工学-机械工程] 071002[理学-动物学] 

基　　金：This paper was supported by a grant from the National High Technology Research and Development Program of China （863 program） （No. 2012AA020504） and the National Natural Science Foundation of China （Key Program） （No. 81027004）. We thank the authors in our list of references for their excellent work  which led to the main content and structure of this review. 

主　　题：microtomy 3D imaging computer-assisted image processing 3D printing tissue scaffold 

摘      要：Three-dimensional （3D） image reconstruction technologies can reveal previously hidden microstruc-tures in human tissue. However, the lack of ideal, non-destructive cross-sectional imaging techniques is still a problem. Despite some drawbacks, histological sectioning remains one of the most powerful methods for accurate high-resolution representation of tissue structures. Computer technologies can produce 3D representations of interesting human tissue and organs that have been serial-sectioned, dyed or stained, imaged, and segmented for 3D visualization. 3D reconstruction also has great potential in the fields of tissue engineering and 3D printing. This article outlines the most common methods for 3D tissue section reconstruction. We describe the most important academic concepts in this field, and provide critical explanations and comparisons. We also note key steps in the reconstruction procedures, and highlight recent progress in the development of new reconstruction methods.