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Marginal zone B cells are naturally reactive to collagen type ii and are involved in the initiation of the immune response in collagen-induced arthritis

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Cellular & Molecular Immunology 2011年第4期8卷 296-304页

作者： Cecilia Carnrot Kajsa E Prokopec Kristina Rasbo Mikael CI Karlsson Sandra Kleinau Department of Cell and Molecular Biology Uppsala UniversityUppsalaSweden Department of Medicine Unit of Clinical Allergy ResearchThe Karolinska InstituteSolnaSweden

Antibodies against type ii collagen(Cii)are essential for development of collagen-induced arthritis(CIA),but how and where the B-cell response to Cii is initiated is not fully *** show here that naive DBA/1 mice display naturally reactive IgM and IgG anti-Cii producing B cells prior to *** Cii-reactive B cells were observed in the spleen and recognized as marginal zone(MZ)B *** Cii immunization,Cii-specific B cells expanded rapidly in the spleen,in contrast to the lymph nodes,with the initial response derived from MZ B cells and later by follicular(FO)B *** was evident despite that the MZ B cells were subject to stringent tolerance mechanisms by having a greater Fc gamma receptor iib expression than the FO B ***,the MZ B cells migrated to the FO areas upon immunization,possibly providing antigen and activating FO T cells and subsequently FO B ***,around CIA onset increased numbers of IgG anti-Cii producing FO B cells was seen in the spleen,which was dominated by IgG2a-and IgG2b-positive *** data demonstrate that Cii-reactive MZ B cells are present before and expand after Cii immunization,suggesting an initiating role of MZ B cells in the development of CIA.

关键词： arthritis B cells collagen type ii ELISpot marginal zone mice

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Effects of adeno-associated virus (AAV) of transforming growth factors β_1 and β_3 (TGFβ_(1,3)) on promoting synthesis of glycosaminoglycan and collagen type ii of dedifferentiated nucleus pulposus (NP) cells

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Science China(Life Sciences) 2007年第5期50卷 605-610页

作者： SAI JiaMing HU YouGu WANG DeChun Orthopaedic Laboratory The Orthopaedics and Traumatology Hospital Qingdao 266003 China Department of Orthopedic Surgery Affiliated Hospital of Medical College Qingdao University Qingdao 266003 China Department of Orthopedic Surgery Affiliated Hospital of Medical College Qingdao University Qingdao 266003 China

The effects of AAV-TGF beta_1 and AAV-TGF beta_3 on promoting synthesis ofglycosaminoglycan and collagen type ii of dedifferentiated rabbit lumbar disc NP cells were studiedin this *** rabbit lumbar disc NP cells were isolated and *** earlier and laterdedifferentiated NP cells were established by *** AAV transfection efficiency todedifferentiated NP cells was analyzed with AAV-EGFP in *** dedifferentiated NP cells weretransfected by AAV-TGFp,or AAV-TGF beta_3,their biological effects on promoting synthesis ofglycosaminoglycan or collagen type ii were detected and compared by the methods of 35S incorporationor *** experimental results showed that AAV could transfect efficiently the earlierdedifferentiated NP cells,but its transfection rate was shown to be at a low level to the laterdedifferentiated NP *** AAV-TGF beta_1,and AAV-TGF beta_3 could promote the earlierdedifferentiated NP cells to synthesize glycosaminoglycan and collagen type ii,and the effect ofAAV-TGFp,was better than that of AAV-TGF beta_*** the later dedifferentiated NP cells,the AAV-TGFbeta_3 could promote their synthesis,but AAV-TGFp,could slightly inhibit ***,AAV-TGFp,and AAV-TGF beta_3 could be used for the earlier dedifferentiated NPcells,and the TGF beta_3 could be used as the objective gene for the later dedifferentiated NPcells.

关键词： adeno-associated virus (AAV) transforming growth factor-beta_1 (TGF beta_1) transforming growth factor-beta_3 (TGFp3) glycosaminoglycan collagen type ii gene transfection

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The Use of Bone Morphogenetic Protein-7 and Resveratrol in collagen type ii of Articular Cartilage

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Journal of Pharmacy and Pharmacology 2016年第5期4卷 199-211页

作者： Molaba Gloria Mmadira Motaung Shirley Keolebogile Tshwane University of Technology Faculty of Science Department of Biomedical Sciences Private Bag X 680 Pretoria SouthAfrica 0001

This study aimed to investigate the effects of resveratrol and bone morphogenetic protein 7 on type ii collagen from superficial and middle zone of porcine articular chondrocytes. Articular cartilage was isolated from dissected porcine knee joint n = 12. Isolated cells were plated as monolayers at a density of 1 × 105 cells/well in 12-well culture plates and incubated at 37℃ in a humid atmosphere of 5% carbon dioxide and 95% air. Cell cultures were treated for four days with various concentrations of bone morphogenetic protein-7 and resveratroL Cells were then collected and analysed for collagen type ii expression by real time polymerase chain reaction and protein level quantification by enzyme-linked immunosorbent assay. Cartilage tissue sections were localised for collagen type ii by immunohistochemistry. Moreover, resveratrol and bone morphogenetic protein-7 effects on cartilage matrix contents were analysed by histology. Resveratrol and bone morphogenetic protein-7 stimulates expression of collagen type ii mRNA and protein level accumulation in the surface zone and middle zone at 50μM ＋ 300 ng/ml （RSV ＋ BMP-7）. Immunohistochemistry results confirmed the presence of collagen type ii on articular cartilage. Histological tissue sections confirmed that chondrocytes were obtained from different zones of articular cartilage. The study suggests that a combination of bone morphogenetic protein-7 and resveratrol up-regulate the expression and synthesis of collagen type ii.

关键词： Articular cartilage osteoarthritis collagen type ii resveratrol bone morphogenetic protein-7.

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Ultra-small superparamagnetic iron oxide nanoparticles for intra-articular targeting of cartilage in early osteoarthritis

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Regenerative Biomaterials 2023年第1期10卷 1111-1121页

作者： Jun Wu Changqiang Wu Zhongyuan Cai Haojie Gu Li Liu Chunchao Xia Su Lui Qiyong Gong Bin Song Hua Ai Institute for Disaster Management and Reconstruction Sichuan UniversityChengdu 610207China Medical Imaging Key Laboratory of Sichuan Province School of Medical ImagingNorth Sichuan Medical CollegeNanchong 637000China National Engineering Research Center for Biomaterials Sichuan UniversityChengdu 610064China Department of Radiology West China HospitalSichuan UniversityChengdu 610041China Department of Radiology Huaxi MR Research Center(HMRRC)Frontiers Science Center for Disease-Related Molecular NetworkState Key Laboratory of BiotherapyWest China HospitalSichuan UniversityChengdu 610041China Functional and Molecular Imaging Key Laboratory of Sichuan Province Key Laboratory of Transplant Engineering and ImmunologyNHCResearch Unit of PsychoradiologyChinese Academy of Medical SciencesChengdu 610064China Department of Radiology West China Xiamen Hospital of Sichuan UniversityFujianXiamen 361000China Department of Radiology Sanya People’s HospitalHainanSanya 572000China

Early diagnosis of osteoarthritis(OA)is critical for effective cartilage ***,lack of blood vessels in articular cartilage poses a barrier to contrast agent delivery and subsequent diagnostic *** address this challenge,we proposed to develop ultra-small superparamagnetic iron oxide nanoparticles(SPIONs,4 nm)that can penetrate into the matrix of articular cartilage,and further modified with the peptide ligand WYRGRL(particle size,5.9 nm),which allows SPIONs to bind to type ii collagen in the cartilage matrix and increase the retention of *** ii collagen in the cartilage matrix is gradually lost with the progression of OA,consequently,the binding of peptide-modified ultra-small SPIONs to type ii collagen in the OA cartilage matrix is less,thus presenting different magnetic resonance(MR)signals in OA group from the normal *** introducing the AND logical operation,damaged cartilage can be differentiated from the surrounding normal tissue on T1 and T2 AND logical map of MR images,and this was also verified in histology ***,this work provides an effective strategy for delivering nanosized imaging agents to articular cartilage,which could potentially be used to diagnosis joint-related diseases such as osteoarthritis.

关键词： early osteoarthritis magnetic resonance imaging superparamagnetic iron oxide collagen type ii targeting peptide

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