Effects of adeno-associated virus (AAV) of transforming growth factors β_1 and β_3 (TGFβ_(1,3)) on promoting synthesis of glycosaminoglycan and collagen type II of dedifferentiated nucleus pulposus (NP) cells

作     者：SAI JiaMing HU YouGu WANG DeChun 

作者机构：Orthopaedic Laboratory The Orthopaedics and Traumatology Hospital Qingdao 266003 China Department of Orthopedic Surgery Affiliated Hospital of Medical College Qingdao University Qingdao 266003 China Department of Orthopedic Surgery Affiliated Hospital of Medical College Qingdao University Qingdao 266003 China 

出 版 物：《Science China(Life Sciences)》 (中国科学（生命科学英文版）)

年 卷 期：2007年第50卷第5期

页      面：605-610页

核心收录：

学科分类：07[理学] 08[工学] 

基　　金：Supported by the National Natural Science Foundation of China (Grant No. 30271318) 

主　　题：adeno-associated virus (AAV) transforming growth factor-beta_1 (TGF beta_1) transforming growth factor-beta_3 (TGFp3) glycosaminoglycan collagen type II gene transfection 

摘      要：The effects of AAV-TGF beta_1 and AAV-TGF beta_3 on promoting synthesis ofglycosaminoglycan and collagen type II of dedifferentiated rabbit lumbar disc NP cells were studiedin this *** rabbit lumbar disc NP cells were isolated and *** earlier and laterdedifferentiated NP cells were established by *** AAV transfection efficiency todedifferentiated NP cells was analyzed with AAV-EGFP in *** dedifferentiated NP cells weretransfected by AAV-TGFp,or AAV-TGF beta_3,their biological effects on promoting synthesis ofglycosaminoglycan or collagen type II were detected and compared by the methods of 35S incorporationor *** experimental results showed that AAV could transfect efficiently the earlierdedifferentiated NP cells,but its transfection rate was shown to be at a low level to the laterdedifferentiated NP *** AAV-TGF beta_1,and AAV-TGF beta_3 could promote the earlierdedifferentiated NP cells to synthesize glycosaminoglycan and collagen type II,and the effect ofAAV-TGFp,was better than that of AAV-TGF beta_*** the later dedifferentiated NP cells,the AAV-TGFbeta_3 could promote their synthesis,but AAV-TGFp,could slightly inhibit ***,AAV-TGFp,and AAV-TGF beta_3 could be used for the earlier dedifferentiated NPcells,and the TGF beta_3 could be used as the objective gene for the later dedifferentiated NPcells.