检索结果-南通市图书馆

Effect of clenbuterol hydrochloride on the in vitro Development of Mouse Embryo

Journal of Reproduction and Contraception 2002年第3期13卷 146-151页

作者： Li-huaLU Wei-jieZHU JingLI CenterforReproductiveImmunologyResearch JinanUniversityGuangzhou510632China Dept.ofPathophysiology MedicalCollegeJinanUniversityGuangzhou510632China

To investigate the effect of clenbuterol hydrochloride on the in vitro development of both 1 cell and 2 cell mouse embryos. Methods The cultural systems of both 1 cell and 2 cell mouse embryo were used to determine the effect of clenbuterol hydrochloride at doses of 1 ng/mL, 3 ng/mL, and 10 ng/mL on developmental rates of mouse embryos. Results When 1 cell embryos cultured with 1 ng/mL of clenbuterol hydrochloride, developmental rates from the 4 cell stage to blastocyst stage were significantly lower than those in the control group (Pclenbuterol hydrochloride, obvious differences in developmental rates were not found between the 2 cell embryo group and the control (P>0.05). However, at levels of 3 ng/mL and 10 ng/mL, significant decrease of developmental rates in 2 cell embryos was observed from the 4 cell and from the 8 cell stage, respectively (Pclenbuterol hydrochloride appeared to have more granules, fragments and degeneration than those in the control. Conclusion clenbuterol hydrochloride has a toxic effect on the mouse embryos, and the effect is in a dose dependent. 1 cell mouse embryos cultured with clenbuterol hydrochloride could be easily inhibited at 2 cell stage, but the effect of clenbuterol hydrochloride on development of the late 2 cell embryos would be reduced.

关键词： clenbuterol hydrochloride mouse embryo in vitro culture

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

High performance liquid chromatographic separation of eight drugs collected in Chinese Pharmacopoeia 2010 on amylose ramification chiral stationary phase

引用

Acta Pharmaceutica Sinica B 2012年第5期2卷 527-533页

作者： Yan Wang Ying Zhou Chao Ma Beibei Yang Ru Feng Yiying Zhang Jie Fu Wenjing Chen Yupeng Sun Jingyi Ma Qiming Zhang Yulin Deng Yukui Zhang Wenyi He School of Life Beijing Institute of TechnologyBeijing 100081China State Key Laboratory of Bioactive Substances and Functions of Natural Medicines Institute of Materia MedicaChinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100050China National Institute for the Control of Pharmaceutical and Biological Products Beijing 100050China

The enantiomers separation of ei ght pharmaceutical racemates collected in Chinese Pharmacopoeia 2010(Ch.P2010),including nitrendipine,felodipine,omeprazole,praziquantel,sulpiride,clenbuterol hydrochloride,verapamil hydrochloride and chlorphenamine maleate,was performed on chiral stationary phase of amylose ramification by high performance liquid chromatography(HPLC)on Chiralpak AD-H column and Chiralpak AS-H column with the mobile phase consisted of isopropanol and *** detection wavelength and the flow rate were set at 254 nm and 0.7 mL/min,*** effects of proportion of organic additives,alcohol displacer and temperature on the separation were *** results indicated that eight chiral drugs were separated on chiral stationary phase of amylase ramification in normal phase chroma tographic *** chromatographic retention and resolution of enantiomers were adjusted by factors,including the changes of the concentration of alcohol displacer in mobile phase,organic alkaline modifier and column *** was shown that the resolution was improved with reducing concentration of alcohol *** the concentration of organic alkaline modifier was 0.2%,the resolution and the peak shape were fairly *** racemates mentioned above had the best resolution at column temperature of 25℃.The best temperature should be kept unchanged in the process of separation so as to obtain stable separation results.

关键词： Normal phase HPLC Chiral stationary phase Optical enantiomers Amylose ramification Nitrendipine Felodipine Omeprazole Praziquantel Sulpiride clenbuterol hydrochloride Verapamil hydrochloride Chlorphenamine maleate

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：