Effect of Clenbuterol Hydrochloride on the in vitro Development of Mouse Embryo

作     者：Li-huaLU Wei-jieZHU JingLI Li hua LU, Wei jie ZHU, Jing LI * Center for Reproductive Immunology Research, Jinan University, Guangzhou 510632, China * Dept. of Pathophysiology, Medical College, Jinan University, Guangzhou 510632, China

作者机构：CenterforReproductiveImmunologyResearchJinanUniversityGuangzhou510632China Dept.ofPathophysiologyMedicalCollegeJinanUniversityGuangzhou510632China 

出 版 物：《Journal of Reproduction and Contraception》 (生殖与避孕（英文版）)

年 卷 期：2002年第13卷第3期

页      面：146-151页

学科分类：0710[理学-生物学] 07[理学] 071008[理学-发育生物学] 

基　　金：the Natural Science Foundation of Guangdong ProvinceChina 

主　　题：clenbuterol hydrochloride mouse embryo in vitro culture 

摘      要：To investigate the effect of clenbuterol hydrochloride on the in vitro development of both 1 cell and 2 cell mouse embryos. Methods The cultural systems of both 1 cell and 2 cell mouse embryo were used to determine the effect of clenbuterol hydrochloride at doses of 1 ng/mL, 3 ng/mL, and 10 ng/mL on developmental rates of mouse embryos. Results When 1 cell embryos cultured with 1 ng/mL of clenbuterol hydrochloride, developmental rates from the 4 cell stage to blastocyst stage were significantly lower than those in the control group (P0.05). However, at levels of 3 ng/mL and 10 ng/mL, significant decrease of developmental rates in 2 cell embryos was observed from the 4 cell and from the 8 cell stage, respectively (P0.05). Embryos cultured with clenbuterol hydrochloride appeared to have more granules, fragments and degeneration than those in the control. Conclusion Clenbuterol hydrochloride has a toxic effect on the mouse embryos, and the effect is in a dose dependent. 1 cell mouse embryos cultured with clenbuterol hydrochloride could be easily inhibited at 2 cell stage, but the effect of clenbuterol hydrochloride on development of the late 2 cell embryos would be reduced.