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The ceRNA Network Indicates Its Immune Function in Peripheral Blood Leukocytes of Half-Smooth Tongue Sole(Cynoglossus semilaevis)After Stimulation with chitosan oligosaccharide

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Journal of Ocean University of China 2024年第6期23卷 1615-1629页

作者： WANG Wenwen WEI Shu CHEN Yadong TAN Suxu SHI Kunpeng ZANG Shaoqing WANG Minmin YANG Teng YANG Yingming CUI Zhongkai SHA Zhenxia Institute of Aquatic Biotechnology College of Life SciencesQingdao UniversityQingdao 266071China State Key Laboratory of Mariculture Biobreeding and Sustainable Goods Yellow Sea Fisheries Research InstituteChinese Academy of Fishery SciencesQingdao 266071China Laboratory for Marine Fisheries Science and Food Production Processes Qingdao Marine Science and Technology CenterQingdao 266237China

As an efficient immunostimulant,chitosan oligosaccharide(COS)can enhance the immunity of teleosts;however,the underlying molecular mechanisms still require *** endogenous RNAs(ceRNAs)are vital regulators in the immune response,but their roles in half-smooth tongue sole(Cynoglossus semilaevis)remain *** this study,for the first time,we studied whole-transcriptome expression profiles and analyzed ceRNA networks in peripheral blood leukocytes of half-smooth tongue sole treated with COS.A total of 19 circRNAs(DE-circRNAs),18 miRNAs(DE-miRNAs)and 50 previously identified lncRNAs(DElncRNAs)were differentially expressed after COS *** DE-lncRNAs and DE-miRNAs targeted numerous immunity-related genes,and were enriched in important pathways,including MAPK and Toll-like receptor signaling pathways,suggesting the immunoregulatory roles of ***,we constructed circRNA-miRNA-mRNA and lncRNA-miRNA-mRNA regulatory networks using DE-circRNAs,DE-miRNAs,DE-lncRNAs,and ***,a ceRNA network with immunity-related DEmRNAs was constructed,showing that 3 DE-circRNAs,12 DE-lncRNAs,and 29 DEGs exhibited crosstalk through 9 ***,a DE-miRNA in the ceRNA network,miR-144-3p,was targeted by DE-lncRNA tnrc6a,and negatively regulated the genes of inhibitor of nuclear factor kappa B kinases(IKKs)(ikbkg,ikbkb,and ikbip)and ***,ikbkb,and c3ar1 were significantly up-regulated in macrophages stimulated by *** could be inferred that ncRNAs participated in the immune and inflammatory response by acting as ceRNAs after COS stimulation in *** findings indicate that COS could enhance the immunity of teleosts by regulating ncRNAs,and lay the foundation for further practical application of COS in aquaculture.

关键词： ceRNA circRNA miRNA half-smooth tongue sole peripheral blood leukocytes chitosan oligosaccharide

Effects of chitosan oligosaccharide-nisin conjugates formed by Maillard reaction on the intestinal microbiota of high-fat diet-induced obesity mice model

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Food Quality and Safety 2019年第3期3卷 169-177页

作者： Xiaojie Zheng Xin Zhang Chunhua Xiong School of Food Science and Biotechnology Zhejiang Gongshang UniversityHangzhou Department of Food Science and Engineering School of Marine SciencesNingbo UniversityP.R.China

Objectives:The goal of this study was to evaluate the modulatory effect of chitosan oligosaccharide-nisin conjugate(CON-C)on intestinal microbiota of human flora-associated(HFA)mice and also reveal its effect towards the high-fat diet(HFD)-induced *** chitosan oligosaccharides and nisin showed great potential in modulating the intestinal microbiota,so it is worth to explore whether the modulation effect of chitosan oligosaccharide could be improved by covalently binding with *** and Methods:CON-C was prepared by heating the mixed solution of chitosan oligosaccharide and nisin at 80℃ and pH 2.0 for *** structure of CON-C were analyzed by Fourier transform-infrared spectroscopy(FT-IR)and X-ray diffraction(XRD).The CON-C’s anti-obesity effect and modulatory effect toward intestinal microbiota were analyzed using human flora-associated(HFA)mice ***:CON-C could alleviated HFD-induced gut dysbiosis,by significantly decreasing the numbers of Bifidobacterium and Lactobacillus/Enterococcus spp.,and increasing the numbers of Bacteroides-Prevotella and Clostridium ***-C could also enriched the most differentially expressed genes through KEGG pathways of biosynthesis of amino acids,two-component system,and ATP binding cassette(ABC)***:The improved therapeutic effect of CON-C against HFD-induced obesity has been approved,and hence,CON-C has a great potential to be utilized as a functional food ingredient in reducing body weight.

关键词： chitosan oligosaccharide nisin Maillard reaction modulatory effect intestinal microbiota

Preparation,characterization,and antibacterial activity of oleic acid-grafted chitosan oligosaccharide nanoparticles

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Frontiers in Biology 2009年第3期4卷 321-327页

作者： Lu HUANG Xiaojie CHENG Chengsheng LIU Ke XING Jing ZHANG Gangzheng SUN Xiaoyan LI Xiguang CHEN College of Marine Life Science Ocean University of ChinaQingdao 266003China

An oleic acid-grafted chitosan oligosaccharide(CSO-OA)with different degrees of amino substitution(DSs)was synthesized by the 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide(EDC)-mediated coupling *** transform infrared spectroscopy(FT-IR)suggested the formation of an amide linkage between amino groups of chitosan oligosaccharide and carboxyl groups of oleic *** critical aggregation concentrations(CACs)of CSO-OA with 6%,11%,and 21%DSs were 0.056,0.042,and 0.028 mg·mL^(-1),*** prepared with the sonication method were characterized by means of transmission electron microscopy(TEM)and Zetasizer,and the antibacterial activity against Escherichia coli and Staphylococcus aureus was *** results showed that the CSO-OA nanoparticles were in the range of 60-200 nm with satisfactory structural *** particle size slightly decreased with the increase of DS of *** antibacterial trial showed that the nanoparticles had good antibacterial activity against *** and ***.

关键词： chitosan oligosaccharide nanoparticles antimicrobial activity

Synthesis of chitosan oligosaccharide selenium and its antitumor activity

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Traditional Medicine Research 2024年第10期9卷 10-20页

作者： Yu-Mei Chen Yan Yu Chen Yin-Yin Li Yue Bai Yang Dong Bing-Qiang Zhang Meng-Meng Chen Xi-Feng Zhang Jing Liu College of Veterinary Medicine Qingdao Agricultural UniversityQingdao 266100China Qingdao Restore Biotechnology Co. Ltd.Qingdao 266111China Key Laboratory of Cancer and Immune Cells of Qingdao Qingdao 266111China Analytical&Testing Center of Qingdao Agricultural University Qingdao 266100China

Background:Polysaccharides have various biological activities;the complexation of polysaccharides with trace element ions can produce synergistic effects,improving the original biological activities of sugars and trace ***:The preparation process of chitosan oligosaccharide selenium(COSSe)was optimized by the response surface method,followed by a detailed analysis of the resultant compound’s *** anti-cancer activity of COSSe was studied using the human ovarian cancer cell line SKOV3 as a cell ***:The prepared COSSe response surface was well predicted,indicating successful chitosan oligosaccharide binding with *** surface method analyses identified the optimal synthesis conditions for COSSe:the reaction time of 5.08 h,the reaction temperature of 71.8°C,and mass ratio(Na2SeO3:chitosan oligosaccharide)of *** the optimal conditions,the final product,the selenium content,reached 1.302%.The results of cell experiments showed that COSSe significantly inhibited SKOV3 proliferation in a concentration-dependent ***-seq results showed that chitosan oligosaccharide and COSSe significantly modulated the expression of genes’DNA metabolic processes and cell cycle in SKOV3 *** enrichment analysis showed the inhibition of the cell cycle,and the results of flow cytometry showed that SKOV3 cells increased in the S phase and decreased in the G2/M phase,with a noted suppression in the protein expression of cyclin-dependent kinase 2(CDK2)and cyclin A1(CCNA1).Conclusion:COSSe has a stronger effect than chitosan oligosaccharide,leading to the arrest of the cell cycle in the S ***,COSSe may be an effective candidate for the treatment of ovarian cancer.

关键词： chitosan oligosaccharide chitosan oligosaccharide selenium ovarian cancer SKOV3 RNA-seq

chitosan oligosaccharide Promotes Osteoclast Formation in Vitro by Stimulating the Activation of MAPK and AKT Signaling Pathways

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Chitosan Oligosaccharide Promotes Osteoclast Formation in Vi...

Specific Targeting MRI of chitosan oligosaccharide Modified Fe_(3)O_(4) Nanoprobe on Macrophage and the Inhibition of Macrophage Foaming Induced by ox-LDL

2018年浙江省医学会骨质疏松与骨矿盐疾病分会学术年会暨骨质疏松症和骨质疏松性骨折诊治进展专题研讨会

作者： Bing-Li Bai Zhong-Jie Xie She-Ji Weng Zong-Yi Wu Hang Li Zhou-Shan Tao Viraj Boodhun De-Yi Yan Zi-Jian Shen Jia-Hao Tang Lei Yang Department of Orthopedics Surgery The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University Department of Trauma Orthopedics The first Affiliated Hospital of Wannan Medical CollegeYijishan Hospital

chitosan oligosaccharide(COS) has been widely used for the systemic treatment of clinical diseases such as bone tissue ***,its influence on osteoclast formation,which plays a critical role in bone homeostasis,has never been *** aim of this study was to investigate the effect of chitosan oligosaccharide on differentiation of *** cell counting kit-8,tartrate-resistant acid phosphatase staining,reverse transcription-quantitative polymerase chain reaction assay and western blot analysis,we demonstrated that chitosan oligosaccharide cannot inhibit RANKL-induced osteoclast precursor proliferation but does promote osteoclast differentiation by stimulating the activation of p38/mitogen-activated protein kinase(MAPK),c-Jun N-terminal kinase(JNK)/MAPK,extracellular signal-regulated kinase(ERK)/MAPK and protein kinase B(AKT) without affecting nuclear factor kappaB(NF-kB) signaling *** on the promoting effect of chitosan oligosaccharide on osteoclast differentiation,we suggest that this property of chitosan oligosaccharide may have potential detrimental effect on bone homeostasis.

关键词： chitosan oligosaccharide osteoclast p38 c-Jun N-terminal kinase extracellular signal-regulated kinase protein kinase B

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Journal of Advances in Medicine Science 2021年第2期4卷 20-30页

作者： Xu Cao Ke Ma Yuhao Tao Deyang Xi Fangyu Hu Jingjing Li School of Medical Imaging Xuzhou Medical UniversityXuzhouJiangsu221006China Department of Radiology the Affiliated Hospital of Xuzhou Medical UniversityXuzhouJiangsu221002China

Atherosclerosis(AS)is a primary cause of morbidity and mortality all over the *** imaging techniques can enable early localization and diagnosis of atherosclerosis *** newly developed chitooligosaccharides(CSO)is considered to be capable of target mannose receptors on the surface of macrophages and to inhibit foam cell *** we present a targeting magnetic resonance imaging(MRI)nanoprobe,which was successfully constructed with polyacrylic acid(PAA)modified nanometer iron oxide(Fe_(3)O_(4))as the core,and coating with CSO molecules,possessing the abilities of targeted MRI and specifically inhibition of the formation of foamy macrophages in the atherosclerotic *** experimental results showed that the distributions of PAA-Fe_(3)O_(4) and CSO-PAA-Fe_(3)O_(4) were uniform and the corresponding sizes were about 5.93 nm and 8.15 nm,*** Fourier transform infrared spectra(FTIR)testified the CSO was coupled with PAA-Fe_(3)O_(4) *** coupled with CSO,the r1 of PAA-Fe_(3)O_(4) was increased from 5.317 mM s-1 to 6.147 mM s-1,indicating their potential as MRI contrast *** Red O staining and total cholesterols(TC)determination showed that CSO-PAA-Fe_(3)O_(4) could significantly inhibit the foaming process of RAW264.7 cells induced by oxidatively modified low density lipoprotein(ox-LDL).In vitro cellular MRI displayed that,compared with PAA-Fe_(3)O_(4),CSO-PAA-Fe_(3)O_(4) could lower the T1 relaxation time of RAW264.7 cells *** summary,construction of CSO-PAA-Fe_(3)O_(4) nanoprobe in this study could realize the targeted MRI of macrophages and inhibition of ox-LDL induced macrophage foaming *** will provide a new avenue in the diagnosis and treatment of AS.

关键词： chitosan oligosaccharide Iron oxide Macrophages Atherosclerosis Magnetic resonance imaging

The effects of chitosan oligosaccharide on the activation of murine spleen CD11c+ dendritic cells via Toll-like receptor 4

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The effects of Chitosan oligosaccharide on the activation of...

Research on Protective Effect of chitosan oligosaccharide on SH-SY5YCells

中国化学会·2016全国多糖研讨会

作者： Yibing Dang Sheng Li Wenxia Wang Shujing Wang Mingming Zou Yanjie Guo Jianhui Fan Yuguang Du Jianing Zhang Department of Biochemistry Institute of Glycobiology Dalian Medical University Department of Biotechnology Dalian Institute of Chemical Physics CAS School of Life Science and Medicine Dalian University of Technology

＜正＞To investigate the effects of chitosan oligosaccharide（COS）on dendritic cells（DCs）and the role of Toll-like receptor 4（TLR4）in this immune process,Murine spleen CD11c+dendritic cells（SDCs）were isolated and cultured with S-COS and B-COS with polymeration degree of 3-7 and7-16,*** results showed that B-COS up-regulated the expressions of MHCII and CD86 on SDCs,promoted the secretion of TNF-αfrom *** treated with B-COS

关键词： chitosan oligosaccharide Spleen dendritic cell TLR4 immune response

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Research on Protective Effect of Chitosan Oligosaccharide on...

Synthesis and in vitro Evaluation of Manganese（Ⅱ）-porphyrin Modified with chitosan oligosaccharides as Potential MRI Contrast Agents

中国生物化学与分子生物学会2016年全国学术会议

作者： DAI Xue-ling CHANG Ping HUANGHan-Chang WUXue-ling LI Xiao-xiao JIANG Zhao-feng Beijing Key laboratory of Bioactive Substances and Functional Food Research Institute for Science and Technology of Functional Food Beijing Union University

Oxidative damage is one of the important causes leading to neurodegenerative diseases including Alzheimer’s disease(AD).To investigate the protective effect of chitosan oligosaccharide(COS)on nerve cells,we employed hydrogen peroxide as oxidative stress sources to study the neuroprotective effect of various concentration of COS against oxidative stress mediated

关键词： chitosan oligosaccharide hydrogen peroxide antioxidation

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Chemical Research in Chinese Universities 2014年第4期30卷 549-555页

作者： LIU Yun HUANG Yan BOAMAH Peter Osei ZHANG Qi LIU Yuanyuan HUA Mingqing School of Chemistry and Chemical Engineering Jiangsu University Zhenjiang 212013 P. R. China Hainan Provincial Key Lab of Fine Chemistry Hainan University Haikou 570228 P. R. China

Mn-TCPP-CSn（n=6,1 1,20） as a type of potential magnetic resonance imaging（MRI） contrast agents were synthesized via manganese（Ⅱ） meso-tetra（4-carboxyphenyl） porphyrin（Mn-TCPP） modified with chitosan oligosaccharides（CSn）.Experimental data of infared（IR）,UV-Vis,MS,inductively coupled plasma-atomic emission spectrometer（ICP-AES） and size exclusion chromatography evidenced the formation of Mn-TCPP-CSn-The stability results show that Mn-TCPP-CSn in aqueous solution was stable enough to prevent Mn（Ⅱ） ions from *** magnetic properties in vitro indicate that Mn-TCPP-CS20 possesses higher longitudinal relaxivity（r1=10.38 L·mmol^-1·s^-1） in aqueous solution than unmodified porphyrin Mn-TCPPNa4[manganese（Ⅱ） meso-tetra（4-carboxyphenyl） porphyrin,tetrasodium salt]（r1=5.10 L·mmol^-1·s^-1） and the commercial contrast agent Gd-DTPA（r1=4.05 L·mmol^-1·s^-1）.The preliminary T1-weighted flash image studies in vitro show that the contrast and the imaging signal of Mn-TCPP-CSn were superior to those of Mn-TCPPNa4 and Gd-DTPA under the same *** 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide（MTT） assay shows that Mn-TCPP-CSn has a good *** addition,the thermodynamical parameters（ΔH〈0,ΔS〈0,ΔG〈0） of Mn-TCPP-CSn bound to bovine serum albumin（BSA） show that Mn-TCPP-CSn could bind to BSA spontaneously,where the binding complex was stabilized mainly by van der Waals interactions and hydrogen *** results suggest that Mn-TCPP-CSn have the advantage of becoming a potential MRI contrast agent.

关键词： MRI contrast agent Porphyrin chitosan oligosaccharide Relaxivity T1-Weighted flash image