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T lymphocyte apoptosis induced by CD8ε chimera

Chinese Science Bulletin 1997年第3期42卷 222-227页

作者： David Perkins Cox Terhorst Harvard Medical School BostonMAUSAHarvard Medical SchoolBostonMAUSA

THE T cell repertoire is characterized by an extremely diverse population of different surface receptors (TCR) which participates in highly specific responses to a large number of different antigens and mediate antigen stimulation signals. Variable heterodimers of TCR α/β and γ/δ receptors are associated with invariable CD3γ, δ, ε and ζ proteins, thus forming the TCR/CD3 complex. CD3ε plays an important role in the initiation of TCR resembling and signfal transduction among the octopeptide chains. Stimulation by anti-CD3ε monoclonal

关键词： T lymphocyte apoptosis CD8ε chimera.

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Construction of serial deletants and chimera. of multi-kringle containing molecules and primary analysis of their functions

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Science China(Life Sciences) 1999年第5期42卷 548-553页

作者：宫锋董春娜张咏章杨培吴祖泽贺福初 1. Beijing Institute of Radiation Medicine 100850 Beijing China 2. Beijing Institute of Blood Transfusion Medicine 100850 Beijing China

In comparison of amino acid sequences of 4 kringles of both macrophage stimulating protein (MSP) and hepatocyte growth factor (HGF), consensus motif sequence was determined. According to this consensus sequence, a pair of universal primers were designed. In combination with specific upstream or downstream primer of MSP or HGF respectively, serial fragments containing variant number of kringle (from 1 to 4) can be obtained by once PCR. By ligating the C terminal and N terminal fragments with different combination, serial deletants and chimera. of MSP and HGF were constructed. Sequence analysis showed that the degeneracy for universal primers and the sequences of those constructed deletants and chimera. are desired. Biological assay of these deletants revealed that wild type MSP can inhibit the growth of some tumor cell lines and that kringle 1 of MSP is essential for function as that of HGF.

关键词： kringle HGF MSP deletant chimera.

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Establishment of Embryonic Stem Cell Lines from C57BL/6J Mice and Generation of chimera.

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Developmental and Reproductive Biology 1997年第2期6卷 13-20页

作者：何维高建刚刘晓孙方臻中国科学院发育生物学研究所分子发育生物学实验室北京大学生命科学院

Four embryonic stem (ES) cell lines, designated CE1, CE2, CE3 and CE4, were isolated from C57BL/6J blastocysts. The ratio of normal diploid composition of these cell lines is above 70%. To examine the differentiation potential of the ES cells, the CE2 cells were injected subcutaneously into syngenic mice, and many kinds of differentiated cells were observed on the sections of the teratoma derived from this ES cell line. On the other hand, to test the chimeric ability of the ES cells, the CE2 cells were microinjected into the blastocysts of ICR mice, and a chimera.was obtained among living pups. These results show that CE2 ES cells are pluripotent stem cells, which can differentiate into many kinds of cell types, and can be used as a cell system for further research. 

关键词： C57BL/6J mouse ES cell line establishment chimera.

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