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Establishment of Embryonic Stem Cell Lines from C57BL/6J Mice and Generation of Chimeras

从C57BL/6J小鼠胚胎中分离ES细胞系(英文)

作     者:何维 高建刚 刘晓 孙方臻 

作者机构:中国科学院发育生物学研究所分子发育生物学实验室 北京大学生命科学院 

出 版 物:《Developmental and Reproductive Biology》 (发育与生殖生物学报(英文版))

年 卷 期:1997年第6卷第2期

页      面:13-20页

学科分类:08[工学] 09[农学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

主  题:C57BL/6J mouse ES cell line establishment chimera. 

摘      要:Four embryonic stem (ES) cell lines, designated CE1, CE2, CE3 and CE4, were isolated from C57BL/6J blastocysts. The ratio of normal diploid composition of these cell lines is above 70%. To examine the differentiation potential of the ES cells, the CE2 cells were injected subcutaneously into syngenic mice, and many kinds of differentiated cells were observed on the sections of the teratoma derived from this ES cell line. On the other hand, to test the chimeric ability of the ES cells, the CE2 cells were microinjected into the blastocysts of ICR mice, and a chimera was obtained among living pups. These results show that CE2 ES cells are pluripotent stem cells, which can differentiate into many kinds of cell types, and can be used as a cell system for further research. 

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