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Mitotic motor CENP-E cooperates with PRC1 in temporal control of central spindle assembly

Journal of Molecular Cell Biology 2020年第8期12卷 654-665页

作者： Xu Liu Leilei Xu Junying Li Phil Y.Yao Wanjuan Wang Hazrat Ismail Haowei Wang Bryce Liao Zhihong Yang Tarsha Ward Ke Ruan Jianchun Zhang Quan Wu Ping He Xia Ding Dongmei Wang Chuanhai Fu Zhen Dou Feng Yan Wenwen Wang Xing Liu Xuebiao Yao MOE Key Laboratory for Cellular Dynamics&Anhui Key Laboratory for Chemical Biology CAS Center for Excellence in Molecular Cell ScienceHefei National Science Center for Physical Sciences at Microscale&University of Science and Technology of ChinaHefei 230027China Department of Physiology Morehouse School of MedicineAtlantaGA 30310USA School of Basic Medical Sciences Beijing University of Chinese MedicineBeijing 100029China Department of Biology Duke University DurhamNC 27708USA Institute of ProteoGenomics Beijing 100029China Harvard Medical School BostonMA 02115USA

Error-free cell division depends on the accurate assembly of the spindle midzone from dynamic spindle microtubules to ensure chromatid segregation during metaphase-anaphase ***,the mechanism underlying the key transition from the mitotic spindle to central spindle before anaphase onset remains *** the prevalence of chromosome instability phenotype in gastric tumorigenesis,we developed a strategy to model context-dependent cell division using a combination of light sheet microscope and 3D gastric *** sheet microscopic image analyses of 3D organoids showed that CENP-E inhibited cells undergoing aberrant metaphase-anaphase transition and exhibiting chromosome segregation errors during *** real-time imaging analyses of 2D cell culture revealed that CENP-E inhibited cells undergoing central spindle splitting and chromosome instability *** biotinylated syntelin as an affinity matrix,we found that CENP-E forms a complex with PRC1 in mitotic *** inhibition of CENP-E in metaphase by syntelin prevented accurate central spindle assembly by perturbing temporal assembly of PRC1 to the ***,CENP-E-mediated PRC1 assembly to the central spindle constitutes a temporal switch to organize dynamic kinetochore microtubules into stable midzone *** findings reveal a previously uncharacterized role of CENP-E in temporal control of central spindle *** CENP-E is absent from yeast,we reasoned that metazoans evolved an elaborate central spindle organization machinery to ensure accurate sister chromatid segregation during anaphase and cytokinesis.

关键词： organoids.cell division central spindle CENP-E syntelin PRC1

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Mitotic phosphorylation of PRC1 at Thr470 is required for PRC1 oligomerization and proper central spindle organization

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Cell Research 2007年第5期17卷 449-457页

作者： Joseph Whittaker Department of Anatomy & Neurobiology Morehouse School of Medicine Atlanta GA 30310 USA

During cell division, chromosome segregation is orchestrated by the interaction of spindle microtubules with thecentromere. A dramatic remodeling of interpolar microtubules into an organized central spindle between the separatingchromatids is required for the initiation and execution of cytokinesis. central spindle organization requires mitotic kine-sins, the chromosomal passenger protein complex, and microtubule bundling protein PRC1. PRC1 is phosphorylated byCdc2 at Thr470 and Thr481 during mitosis. However, the functional relevance of PRC1 phosphorylation at Thr470 hasremained elusive. Here we show that expression of the non-phosphorylatable mutant PRC1but not the phospho-mimi-cking mutant PRC1causes aberrant organization of the central spindle. Immunoprecipitation experiment indicatesthat both PRC1and PRC1mutant proteins associate with wild-type PRC1, suggesting that phosphorylationof Thr470 does not alter PRC1 self-association. In addition, in vitro co-sedimentation experiment showed that PRC1binds to microtubule independent of the phosphorylation state of Thr470. Gel-filtration experiment suggested that phos-phorylation of Thr470 promotes oligomerization of PRC1. Given the fact that prevention of the Thr470 phosphorylationinhibits PRC1 oligomerization in vitro and causes an aberrant organization of central spindle in vivo, we propose thatthis phosphorylation-dependent PRC1 oligomerization ensures that central spindle assembly occurs at the appropriatetime in the cell cycle.

关键词： central spindle microtubule oligomerization PRC1 phosphorylation

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Cep57 is required for cytokinesis by facilitating central spindle microtubule organization

Cep57 is required for cytokinesis by facilitating central sp...

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细胞—生命的基础——中国细胞生物学学会2013年全国学术大会·武汉

作者： Runsheng He Qixi Wu Haining Zhou Ning Huang Jianguo Chen Junlin Teng The Key Laboratory of Cell Proliferation and Differentiation of Ministry of The Education and The State Key Laboratory of Bio-membrane and Membrane Bio-engineering College of Life SciencesPeking University The Center for Quantitaive Biology Peking University

Cytokinesis is the final stage of cell division in which the cytoplasm of a cell is divided into two daughter cells after the segregation of genetic material,while the central spindle and midbody are considered to be ... 详细信息

关键词： Cep57 cytokinesis central spindle midbody Tektin1

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