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A Novel Structure of LAPS Array for cell-based biosensor

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稀有金属材料与工程 2006年第S3期 51-54页

作者： Ying Xu~(1,2) Hua Cai~1 Qingjun Liu~1 Lifeng Qin~1 Lijiang Wang~1 Ping Wang~(1,2) (1.biosensor National Special Laboratory,Key Laboratory of Biomedical Engineering of Education Ministry,Department of Biomedical Engineering,Zhejiang University,Hangzhou 310027,China) (2.State Key Lab of Transducer Technology,Chinese Academy of Sciences,Shanghai 200050,China)

关键词： light-addressable potentiometric sensor array LAPS sensitivity cell-based biosensor

A novel multi-functional cell-based microphysiometer

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Progress in Natural Science:Materials International 2006年第8期16卷 808-816页

作者： XU Ying, XU Gaixia, LIU Qingjun, CAI Hua, LI Yan, LI Rong and WANG Ping (biosensor National Special Laboratory, Key Laboratory of Biomedical Engineering of Education Ministry, Department of Biomedical Engineering, Zhejiang University, Hangzhou 310027, China State Key Laboratory of Transducer Technology, Chinese Academy of Sciences, Shanghai 200050, China) [a]biosensor National Special Laboratory Key Laboratory of Biomedical Engineering of Education Ministry Department of Biomedical Engineering Zhejiang University Hangzhou China [b]State Key Laboratory of Transducer Technology Chinese Academy of Sciences Shanghai China

This paper presents a novel multi-functional microphysiometer for simultaneous measurements of several extracellular ion concentrations and action potential measurement in living cells based on MLAPS (multi-light addressable potentiometric sensor). In the microphysiometer, sorts of sensitive membranes are illuminated in parallel with n light sources at working frequencies, and the response amplitudes of each frequency component can be measured on-line by parallel processing algorithm. In the experiments, the relations of the extracellular environmental H+, Na+, K+, Ca2+ under the effects of western medicines (dilantin, phenobarbital sodium) and Chinese drugs (scutellaria, medlar, hemlock parsley) were analyzed, and the effects of several drugs were evaluated. Moreover, the action potential signals of different cell types (cardiac myocytes and neurons) could be measured and analyzed by LAPS. By detecting these parameters, the system can monitor the real-time process of the cell metabolism and action potential, observe the functional responses of different kinds of membrane-bound receptors, and evaluate the activities of drugs.

关键词： multi-functional microphysiometer cell-based biosensor cellular ion concentration measurement cellular action potential.

Neurochip based on Light-addressable Potentiometric Sensor

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稀有金属材料与工程 2006年第A3期35卷 413-416页

作者： Qingjun Liu Hua Cai Ying Xu Lifeng Qin Lijiang Wang Ping Wang biosensor National Special Laboratory Key Laboratory of Biomedical Engineering of Education MinistryDepartment of Biomedical Engineering Zhejiang University Hangzhou 310027 China State Key Laboratory of Transducer Technology Chinese Academy of Sciences Shanghai 200050 China

A novel neurochip based on light addressable potentiometric sensor (LAPS) is *** its light addressable *** problems of the limitations of restricted discrete active sites of current neurochips,such as microelectrode array and field effect transistor array can be settled *** on the theoretical analysis of the interface between cells and LAPS,spontaneously discharges of hippocampal neurons induced by Mg~ 2+)-free media treatment were recorded by *** results demonstrate that this kind of neurochip has potential to monitor electrophysiology of cultured cells in a non-invasive way.

关键词： neurochip cell-based biosensor hippocampal neurons light-addressable potentiometric sensor

Neurochip based on Light-addressable Potentiometric Sensor

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稀有金属材料与工程 2006年第S3期 413-416页

作者： Qingjun Liu~(1,2) Hua Cai~1 Ying Xu~(1,2) Lifeng Qin~1 Lijiang Wang~1 Ping Wang~(1,2) (1.biosensor National Special Laboratory,Key Laboratory of Biomedical Engineering of Education Ministry,Department of Biomedical Engineering,Zhejiang University,Hangzhou 310027,China) (2 State Key Laboratory of Transducer Technology,Chinese Academy of Sciences,Shanghai 200050,China)

A novel neurochip based on light addressable potentiometric sensor （LAPS） is *** its light addressable *** problems of the limitations of restricted discrete active sites of current neurochips,such as microelectrode array and field effect transistor array can be settled *** on the theoretical analysis of the interface between cells and LAPS,spontaneously discharges of hippocampal neurons induced by Mg~ 2+)-free media treatment were recorded by *** results demonstrate that this kind of neurochip has potential to monitor electrophysiology of cultured cells in a non-invasive way.

关键词： neurochip cell-based biosensor hippocampal neurons light-addressable potentiometric sensor

A light-addressable microfluidic device for label-free functional assays of bioengineered taste receptor cells via extracellular recording

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Biophysics Reports 2019年第2期5卷 73-79页

作者： Liping Du Wei Chen Yulan Tian Ping Zhu Jian Wang Wen Cai Chunsheng Wu Institute of Medical Engineering Department of Biophysics School of Basic Medical Sciences Health Science Center Xi'an Jiaotong University

The functional assay of chemical sensitive cells is of great importance for the preparation of sensitive elements towards the development of cell-based biosensors for chemical sensing. In this study, a novel light-addressable microfluidic device was developed by the integration of a light-addressable potentiometric sensor(LAPS) with a microfluidic chip for label-free functional assays of bioengineered taste receptor cells via extracellular recording. Extracellular potential changes of single bioengineered cells were recorded by LAPS. Microfluidic chip was capable of providing stable microenvironments for cell measurements with a well-defined concentration stimulus. Bioengineered taste receptor cells were utilized as a model of chemical sensitive cells and functional assayed by this light-addressable microfluidic device using bitter stimulation. The results indicate that this microfluidic device can efficiently monitor the membrane potential changes originated from bioengineered taste receptor cells in response to specific bitter stimulation. The bioengineered cells with responsive functions can be easily identified via the analysis on the firing rates of extracellular recording data. The expression of bitter receptors in the bioengineered taste receptor cells was further validated by the immunofluorescent staining results, which proved that the expression of specific bitter receptor was the main contribution to the responsive function of bioengineered cells. This microfluidic device can not only be used for the functional assays of chemical sensitive cells towards chemical sensing, but also suitable to be applied for the research on the chemical signal transduction mechanisms.

关键词： cell-based biosensor Light-addressable potentiometric sensor Microfluidic device Bioengineered taste receptor cell Extracellular recording