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Effect of Down-regulation of MMP-2 by Quercetin on the migration and invasion of Uterine Fibroids cells through the cAMP Signaling Pathway

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Chinese Journal of Biomedical Engineering 2024年第2期33卷 54-61页

作者： LIN Shu-ping ZOU Xiao-fen Longyan First Affiliated Hospital of Fujian Medical University

Objective: To investigate the effect of down-regulation of matrix metalloproteinase-2(MMP-2) by down-regulating the cyclic adenosine monophosphate(cAMP) signaling pathway on the migration and invasion of uterine fibroids ***: A total of 65 female SD rats were randomly divided into five groups after being adaptively fed for 7 d. Except for the blank group, the remaining four groups of rats were injected with 0.05 mg/(100 g·d) estradiol benzoate and 0.5 mg/(100g·d) progesterone into the muscle to establish a uterine fibroids rat model. After successful modeling, the blank group and model group were given 200 mg/(kg·d) physiological saline by gavage,the low-dose group was given 100 mg/(kg·d) mifepristone by gavage, the medium-dose group was given 200 mg/(kg·d) mifepristone by gavage, and the high-dose group was given 300 mg/(kg·d) mifepristone by gavage. After continuous gavage treatment for 21 d,serum and uterine tissues were collected from rats to observe and compare the expression levels of MMP-2 mRNA, TIMP-2 mRNA, estradiol(E2), cAMP signaling pathway related proteins, and the migration and invasive ability of uterine fibroids cells in the five groups of rats. Results: The expression levels of MMP-2 mRNA and E2in the high-dose group were lower than those in the low-dose group and the medium-dose group, and the expression level of TIMP-2 mRNA was higher than that in the low-dose group and the medium-dose group(Pcell migration in a single visual field of rats in the high-dose group was lower than that in the low-dose group and the medium-dose group(Pcell invasion in a single visual field of rats in the high-dose group was lower than that in the low-dose group and the medium-dose group(P<0.05). The mRNA expression levels of cAMP, PDE and PK in the high-dose group were higher than those in the low-dose group, and the mRNA expression levels of AC in the high-dose group were lower than those in the low-dose group(P<0.

关键词： uterine fibroids letrozole cAMP signaling pathway MMP-2 cell migration and invasion

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lncRNACNN3-206 activates intestinal epithelial cell apoptosis and invasion by sponging miR-212, an implication for Crohn’s disease

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World Journal of Gastroenterology 2020年第5期26卷 478-498页

作者： Na Li Rui-Hua Shi Medical School of Southeast University Nanjing 210009Jiangsu ProvinceChina Department of Gastroenterology Zhongda HospitalAffiliated Hospital of Southeast UniversityNanjing 210009Jiangsu ProvinceChina Department of Biomedical Science Mercer University School of MedicineSavannahGA 31404United States

BACKGROUND Statistics indicate that the incidence of Crohn’s disease(CD)is rising in many *** poor understanding on the pathological mechanism has limited the development of effective therapy against this *** studies showed that long noncoding RNAs(lncRNAs)could be involved in autoimmune diseases including CD,but the detailed molecular mechanisms remain *** To identify the differentially expressed lncRNAs in the intestinal mucosa associated with CD,and to characterize their pathogenic role(s)and related *** The differential expression of lncRNAs was screened by high-throughput RNA sequencing,and the top candidate genes were validated in an expanded cohort by real-time *** regulatory network was predicted by bioinformatic software and competitive endogenous RNA analysis,and was characterized in Caco-2 and HT-29 cell culture using methods of cell transfection,real-time PCR,Western blotting analysis,flow cytometry,and cell migration and invasion ***,these findings were confirmed in vivo using a CD animal *** The 3'end of lncRNACNN3-206 and the 3’UTR of Caspase10 contain highaffinity miR212 binding ***3-206 expression was found to be significantly increased in intestinal lesions of CD *** of the lncRNACNN3-206-miR-212-Caspase10 regulatory network led to increased apoptosis,migration and invasion in intestinal epithelial *** of lncRNACNN3-206 expression alleviated intestinal mucosal inflammation and tissue damage in the CD mouse *** lncRNACNN3-206 may play a key role in CD ***3-206 could be a therapeutic target for CD treatment.

关键词： Crohn’s disease Microarray lncRNACNN3-206 Gene regulation cell migration and invasion miR-212

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Mechanism of SNHG12 in Regulating Human Angiostatin Binding Protein Through MicroRNA497 in the migration and invasion of Human Lung Cancer cells

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Proceedings of Anticancer Research 2022年第5期6卷 85-90页

作者： Yang Yu Yan Wang Tianhong Jia Wei Li Xiaoxue Huang Laboratory Department Affiliated Hospital of Hebei UniversityBaoding 050031Hebei ProvinceChina

Objective:To investigate the effect of small nucleolar host gene 12(SNHG12)on the migration and invasion of human lung cancer cells by regulating human angiostatin binding protein through microribonucleic acid(microRNA)-***:A549,H1299,and PC9 cells were cultured in Roswell Park Memorial Institute(RPMI)-1640 medium containing 10%fetal bovine serum,and human bronchial epithelial(HBE)cells were cultured in Dulbecco’s modified eagle medium(DMEM)containing 10%fetal bovine *** incubator conditions were as follows:saturated humidity,37℃,and 5%carbon dioxide(CO2).Results:The gene expressions of small nucleolar host gene 12(SNHG12)in HBE,A549,H1299,and PC9 were 1.00±0.02,5.61±0.42,3.78±0.29,and 3.51±0.23,*** gene expressions of microRNA-497HBE,A549,H1299,and PC9 were 1.00±0.13,0.21±0.04,0.35±0.05,and 0.37±0.06,respectively,with Pcell apoptosis rate in the microRNA-497 group and the microRNA-497+pcDNA3.1 group were significantly higher than those in the miR-NC group,whereas the A value and cell invasion number were significantly lower than those in the miR-negative control(NC)group,with Pcell apoptosis rate in the microRNA-497+SNHG12 group were significantly lower,whereas the A value and cell invasion number were significantly higher,with Pmigration and invasion of human lung cancer cells by regulating human angiostatin binding protein through microRNA-497.

关键词： SNHG12 MicroRNA-497 Lung cancer cell migration and invasion

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Role of TIPE in the development of Hepatocellular Carcinoma

Role of TIPE in the development of Hepatocellular Carcinoma

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2012全国临床微生物与感染免疫学术研讨会

作者： Pan Yingfang~1,Shan Haixia~1,Zhang Zhenyu~1,Sun Wensheng~1,Gao Lifen~1,Ma Chunhong~1,Liang Xiaohong~(1*) Key Laboratory for Experimental Teratology of Ministry of Education and Institute of Immunology,Shandong University School of Medicine,44 Wenhua Xi Road,Jinan,Shandong,250012 P.R.China

Background Tumor necrosis factor(TNF)-alpha-induced protein 8(TNFAIP8 or TIPE),one member of TIPE family,was recently identified to be associated with ***,it is still unknown about the roles of TNFAIP8 inthe development of hepatocellular carcinoma(HCC).Therefore,the present study was designed to characterize the expression pattern of TNFAIP8 inthe HCC and to explore its possible roles in the development and progression of HCC. Methods Firstly,we investigated TIPE expression of cancerous and their paired paracancerous tissues from 98 HCC patients by RT-PCR and immunohistochemistry(IHC) and analyzed its correlation with clinicopathological ***,the effects of TIPE on cell proliferation and migration were examined in cultured HCC cell line by cell growth curve assay and transwell assay ***,we preliminarily explored the mechanisms of TIPE in the inhibited HCC invasion and migration. Results We first analyzed TIPE mRNA expression in 16 HCC ***-PCR analysis showed that 69% (11/16) of patients exhibited decreased TIPE mRNA expression in cancerous tissues when compared with their corresponding paracancerous ***,downregulated TIPE protein level in HCC tissues were also confirmed by IHC analysis in 98 of paired paraffin ***,TIPE expression in HCC tissues was negatively correlated with tumor size,tumor differentiation,TNM stage,and lymphatic and venous ***,we analyzed the biological functions of TIPE in HCC cell *** growth curve assay showed that TIPE siRNA accelerated the growth of both BEL7402 and HepG2 ***,transwell assay demonstrated that TIPE knockdown also promoted cell migration and invasion,while TIPE overexpression played inhibitory ***, Western blot analysis revealed that TIPE knockdown led to epithelial-mesenchymal transition of HCC cell lines by downregulating E-cadherin expression and upregulating N-cadherin and Vimentin expression. Conclusi

关键词： TIPE HCC cell growth cell migration and invasion EMT

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