Effect of Down-regulation of MMP-2 by Quercetin on the Migration and Invasion of Uterine Fibroids Cells through the cAMP Signaling Pathway
作者机构：Longyan First Affiliated Hospital of Fujian Medical University
出 版 物：《Chinese Journal of Biomedical Engineering》 (中国生物医学工程学报(英文版))
年 卷 期：2024年第33卷第2期
页 面：54-61页
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
摘 要：Objective: To investigate the effect of down-regulation of matrix metalloproteinase-2(MMP-2) by down-regulating the cyclic adenosine monophosphate(cAMP) signaling pathway on the migration and invasion of uterine fibroids ***: A total of 65 female SD rats were randomly divided into five groups after being adaptively fed for 7 d. Except for the blank group, the remaining four groups of rats were injected with 0.05 mg/(100 g·d) estradiol benzoate and 0.5 mg/(100g·d) progesterone into the muscle to establish a uterine fibroids rat model. After successful modeling, the blank group and model group were given 200 mg/(kg·d) physiological saline by gavage,the low-dose group was given 100 mg/(kg·d) mifepristone by gavage, the medium-dose group was given 200 mg/(kg·d) mifepristone by gavage, and the high-dose group was given 300 mg/(kg·d) mifepristone by gavage. After continuous gavage treatment for 21 d,serum and uterine tissues were collected from rats to observe and compare the expression levels of MMP-2 mRNA, TIMP-2 mRNA, estradiol(E2), cAMP signaling pathway related proteins, and the migration and invasive ability of uterine fibroids cells in the five groups of rats. Results: The expression levels of MMP-2 mRNA and E2in the high-dose group were lower than those in the low-dose group and the medium-dose group, and the expression level of TIMP-2 mRNA was higher than that in the low-dose group and the medium-dose group(P0.05). The number of cell migration in a single visual field of rats in the high-dose group was lower than that in the low-dose group and the medium-dose group(P0.05). The number of cell invasion in a single visual field of rats in the high-dose group was lower than that in the low-dose group and the medium-dose group(P0.05). The mRNA expression levels of cAMP, PDE and PK in the high-dose group were higher than those in the low-dose group, and the mRNA expression levels of AC in the high-dose group were lower than those in the low-dose group(P0.
同方期刊数据库