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Adaptation of intestine-based microbial functions to bioethanol production

Chinese Journal of Chemical Engineering 2018年第5期26卷 1140-1144页

作者： Zhuojun Ying Xidong Zhang Nanjing Foreign Language School No. 30 East Beijiag Road Nanjing 210008 Jiangsu China

Animal intestine is a favorable habitat to microbes. It facilitates the evolution of dense and diversified microbial communities that are highly active and persistent throughout life span. Here, we stimulate this unique biosystem to develop high-efficient continuous bio-manufacturing processes. The pig small intestine was explored as a novel bioreactor with industrial Saccharornyces cerevisiae for biofuel production. Results showed that the small intestine was a beneficial material for cell adherence. The cells on the intestine exhibited the abilities of self- immobilization, self-duplication and self-repairing. Therefore the intestine-based S. cerevisiae could be continu- ously used for a long time at high metabolic activities. Both the fermentation speed and ethanol yield were im- proved. This study provides valuable insights into the functions of intestine-based biosystem and should inspire the development of bionic industrial processes. Future dissection of the interface mechanism and design of more bionic materials will make bioprocesses more economically favorable and environmentally sustainable.

关键词： Bioethanol cell immobilization Pig intestine Saccharomyces cerevisiae

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Repeated-batch Cultivation of Encapsulated Monascus purpureus by Polyelectrolyte Complex for Natural Pigment Production

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Chinese Journal of Chemical Engineering 2010年第6期18卷 1013-1017页

作者： LIU Jiefeng REN Yiran YAO Shanjing Department of Chemical and Biological Engineering Zhejiang University Hangzhou 310027 China Department of Biotechnology and Food Engineering Maoming College Maoming 525000 China

In general,productions of natural pigment in submerged microorganism culture were much less than that in solid-state fermentation,because the solid-state culture can provide a support carrier for the mycelium. To improve natural pigment production,the cultivation of Monascus purpureus in submerged encapsulated cell was investigated. Monascus purpureus immobilized in polyelectrolyte complex(PEC) microcapsules,which were pre-pared by sodium cellulose sulphate(NaCS) and poly-dimethyl-diallyl-ammonium chloride(PDMDAAC),was a good substitute for submerged cell culture because it mimicked the solid-state environment. The repeated-batch process with encapsulated cells was studied in flasks and a bubble column. The results indicated that the bubble column was more suitable for the encapsulation culture than the shaking flasks because of its good mass transfer performance and minor shear stress on cells. Owing to the protection of the microcapsule's membrane,Monascus purpureus in microcapsules increased approximately three times over that in free cell culture with negligible cell leakage to the medium. The pigment production in the bubble column finally reached 3.82(OD500) ,which was two times higher than in free cell culture. In addition,the duration of each batch was shortened to 15% of that in free cell culture.

关键词： microencapsulation Monascus purpureus natural pigment polyelectrolyte complex NaCS-PDMDAAC capsule cell immobilization

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A new method of immobilizing HEK293 cells on the inner wall of a capillary column as stationary phase for drug screening

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Journal of Chinese Pharmaceutical Sciences 2016年第11期25卷 821-825页

作者：张晓丹李晨朱凯刘燕萌武瑞君任进宇凌笑梅北京大学医学部药学院化学生物学系北京100191

It is a new strategy to immobilize cells on the inner wall of a capillary column and use affinity capillary electrophoresis（ACE） to study receptor-ligand interactions or to screen natural products and compounds synthesized by combinatorial chemistry. In this paper, we developed a new method of immobilizing HEK293 cells on the inner wall of a capillary column. Four important experimental conditions were optimized, including cell injection density, PLL concentration, cell culturing time and sterile processing method. Immobilized cell-coated capillary columns prepared under the optimized experimental conditions exhibited good uniformity, stability and durability, which were suitable for capillary electrophoresis. The method could also be used to immobilize HEK293 cells over-expressing certain membrane receptors on the inner wall of a capillary. In this way, cell-coated capillary columns could be applied to ACE drug screening targeting certain membrane proteins.

关键词： cell immobilization Affinity capillary electrophoresis Molecular interactions Drug screening Stationary phase

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Batch CGTase Production with Free and Immobilized <i>Bacillus firmus</i>Strain 37 in Bovine Bone Charcoal

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Advances in Chemical Engineering and Science 2021年第1期11卷 91-104页

作者： Larissa Albunio Silva Graciette Matioli Gisella Maria Zanin Flavio Faria Moraes Chemical Engineering Department State University of Maringa Maringá Brazil Pharmaceutical Sciences Department State University of Maringa Maringá Brazil

The present study aimed to study the batch production of CGTase (cyclomaltodextrin-glucanotransferase) with Bacillus firmus strain 37 free and immobilized in bovine bone charcoal in batch mode and in a fluidized bed batch reactor, respectively. The bovine bone charcoal is an innovative support material for the immobilization of microorganisms’ producers of enzymes and the use of this microbial support allows its reuse to a significant cost reduction of the process. The batch fermentation with free cells was investigated for 96 h and reached a CGTase activity equal to 0.77 U/mL. When the microorganism was immobilized on bovine bone charcoal (7 g) and cultivated in fluidized bed batch reactor with air supplementation (1 volume of air/volume of medium * minute), the same activity could be achieved in 24 h. The results of enzymatic activity achieved show the potential of CGTase production in a short time with Bacillus firmus strain 37 immobilized in bovine bone charcoal matrix and using air supplementation in the production medium.

关键词： Bacillus firmus CGTase Bone Charcoal Matrix Fluidized Bed Batch Reactor cell immobilization

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