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第十二届全国免疫学学术大会

作者： Cao Xin Xue Yu-Jia Du Jiang-Long Wang Bo-Bo Xu Qiang Ma Xiao-Xia Northwest Minzu University

Zbtb1 is a member of the zinc finger and BTB domain containing protein family of transcriptional factors,which is critical for T cell *** previous study,we found that Zbtb1 can regulate the expression of IL-7 Rα during T cell development of ***-7 signaling is transduced by the IL-7 receptor,which is essential for T cell maturation and *** developmental defects of T cells induced by Zbtb1 mutation,the present study aimed to investigate the regulation of IL-7 Rα gene expression by Zbtb1 ***’re going to overexpress Zbtb1 gene in vitro and in vivo,which can confirm the inhibitory effect of Zbtb1 on IL-7 Rα*** binding of Zbtb1 to cis-element related to IL-7 Raexpression will be identified by Biotin ChIP-seq *** key sites of cis-element for Zbtb1 binding will be mutated by CRISPR-Cas9 technique in a mouse DN thymocyte cell line(Dl).The differential regulation of IL-7 Rαwill be tested in wild type and mutant D1 cell line by overexpression of Zbtb1,which can illustrate the molecular mechanism for IL-7 Rαregulation by *** results for this study will shed light on the function and mechanism of Zbtb1 on T cell development.

关键词： T cell cell development Thymus Cytokine Transcriptional regulation

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Single-cell transcriptomic analysis reveals the developmental trajectory and transcriptional regulatory networks of pigment glands in Gossypium bicki

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Molecular Plant 2023年第4期16卷 694-708页

作者： Yue Sun Yifei Han Kuang Sheng Ping Yang Yuefen Cao Huazu Li Qian-Hao Zhu Jinhong Chen Shujin Zhu Tianlun Zhao College of Agriculture and Biotechnology Zhejang UniversityHangzhou 310058China Agricultural Experiment Station Zhejiang UniversityHangzhou 310058China CSIRO Agriculture and Food GPO Box 1700CanberraACT 2601Australia Institute of Hainan Zhejiang UniversityHangzhou 310058China

Comprehensive utilization of cottonseeds is limited by the presence of pigment glands and its inclusion *** ideal cotton has glandless seeds but a glanded plant,a trait found in only a few Australian wild cotton species,including Gossypium *** of this trait into cultivated species has proved to be *** the biological processes toward pigment gland morphogenesis and the associated underlying molecular mechanisms will facilitate breeding of cultivated cotton varieties with the trait of glandless seeds and glanded *** this study,single-cell RNA sequencing(scRNA-seg)was performed on 12222 protoplasts isolated from cotyledons of germinating *** seeds 48 h after *** into 14 distinct clusters unsupervisedly,these cells could be grouped into eight cell populations with the assistance of known cell marker *** pigment gland cells were well separated from others and could be separated into pigment gland parenchyma cells,secretory cells,and apoptotic *** integrating the pigment gland cell developmental trajectory,transcription factor regulatory networks,and core transcription factor functional validation,we established a model for pigment gland *** this model,light and gibberellin were verified to promote the formation of pigment *** addition,three novel genes,GbiERF114(ETHYLENE RESPONSE FACTOR 114),GbiZAT11(ZINC FINGER OF ARABIDOPSIS THALIANA 11),and GbiNTL9(NAC TRANSCRIPTION FACTOR-LIKE 9),Were found to affect pigment gland ***,these findings provide new insights into pigment gland morphogenesis and lay the cornerstone for future cotton scRNA-seq investigations.

关键词： scRNA-seq pigment gland morphogenesis cell development cotton

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