Single-cell transcriptomic analysis reveals the developmental trajectory and transcriptional regulatory networks of pigment glands in Gossypium bicki
作者机构：College of Agriculture and BiotechnologyZhejang UniversityHangzhou 310058China Agricultural Experiment StationZhejiang UniversityHangzhou 310058China CSIRO Agriculture and FoodGPO Box 1700CanberraACT 2601Australia Institute of HainanZhejiang UniversityHangzhou 310058China
出 版 物：《Molecular Plant》 (分子植物（英文版）)
年 卷 期：2023年第16卷第4期
页 面：694-708页
核心收录：
学科分类：0710[理学-生物学] 071001[理学-植物学] 07[理学]
基 金：supported by the Genetically Modified Organisms Breeding Major Project of China(2016ZX08005005) the National Key Technology R&D Program of China(2016YFD0101404) the China Agricultural Research System(CARS-15-27) the Jiangsu Collaborative Innovation Center for Modern Crop Production,and the National Science Foundation of China(32101764)
主 题：scRNA-seq pigment gland morphogenesis cell development cotton
摘 要：Comprehensive utilization of cottonseeds is limited by the presence of pigment glands and its inclusion *** ideal cotton has glandless seeds but a glanded plant,a trait found in only a few Australian wild cotton species,including Gossypium *** of this trait into cultivated species has proved to be *** the biological processes toward pigment gland morphogenesis and the associated underlying molecular mechanisms will facilitate breeding of cultivated cotton varieties with the trait of glandless seeds and glanded *** this study,single-cell RNA sequencing(scRNA-seg)was performed on 12222 protoplasts isolated from cotyledons of germinating *** seeds 48 h after *** into 14 distinct clusters unsupervisedly,these cells could be grouped into eight cell populations with the assistance of known cell marker *** pigment gland cells were well separated from others and could be separated into pigment gland parenchyma cells,secretory cells,and apoptotic *** integrating the pigment gland cell developmental trajectory,transcription factor regulatory networks,and core transcription factor functional validation,we established a model for pigment gland *** this model,light and gibberellin were verified to promote the formation of pigment *** addition,three novel genes,GbiERF114(ETHYLENE RESPONSE FACTOR 114),GbiZAT11(ZINC FINGER OF ARABIDOPSIS THALIANA 11),and GbiNTL9(NAC TRANSCRIPTION FACTOR-LIKE 9),Were found to affect pigment gland ***,these findings provide new insights into pigment gland morphogenesis and lay the cornerstone for future cotton scRNA-seq investigations.
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