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cell transplantation to replace retinal ganglion cells faces challenges-the Switchboard Dilemma

Neural Regeneration Research 2021年第6期16卷 1138-1143页

作者： Yuan Liu Richard K.Lee Bascom Palmer Eye Institute University of Miami Miller School of MedicineMiamiFLUSA

The mammalian retina displays incomplete intrinsic regenerative capacities;therefore,retina degeneration is a major cause of irreversible blindness such as glaucoma,agerelated macular degeneration and diabetic *** diseases lead to the loss of retinal cells and serious vision loss in the late *** cell transplantation is a great promising novel treatment for these incurable retinal degenerative diseases and represents an exciting area of regenerative *** suitable stem cell sources for transplantation including human embryonic stem cells,induced pluripotent stem cells and adult stem cells have been identified as promising target ***,the retina is an elegant neuronal complex composed of various types of cells with different *** replacement of these different types of cells by transplantation should be addressed *** far,retinal pigment epithelium transplantation has achieved the most advanced stage of clinical trials,while transplantation of retinal neurons such as retinal ganglion cells and photoreceptors has been mostly studied in pre-clinical animal *** this review,we opine on the key problems that need to be addressed before stem cells transplantation,especially for replacing injured retinal ganglion cells,may be used practically for treatment.A key problem we have called the Switchboard Dilemma is a major block to have functional retinal ganglion cell *** use the public switchboard telephone network as an example to illustrate different difficulties for replacing damaged components in the retina that allow for visual *** ganglion cell transplantation is confronted by significant hurdles,because retinal ganglion cells receive signals from different interneurons,integrate and send signals to the correct targets of the visual system,which functions similar to the switchboard in a telephone network-therefore the Switchboard Dilemma.

关键词： cell transplantation optic nerve regeneration photoreceptors retina degeneration retinal ganglion cells stem cells

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Cerebrospinal fluid used as culture medium prior to autologous olfactory ensheathing cell transplantation

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Neural Regeneration Research 2010年第1期5卷 21-27页

作者： Weijiang Wu Qing Lan Hua Lu Aihua Zhu Yunzhao Jiang Ge Chen Guozhen Hui Department of Neurosurgery Wuxi Third People's Hospital Wuxi 214041 Jiangsu Province China Second Affiliated HospitaI Soochow University Suzhou 215004 Jiangsu Province China First Affiliated Hospital Soochow University Suzhou 215006 Jiangsu Province China

BACKGROUND： Cerebrospinal fluid can be an inducer for neural stem cells in vitro, but few studies employ cerebrospinal fluid to culture olfactory ensheathing cells. OBJECTIVE： To investigate the growth of nasal mucosa olfactory ensheathing cells in normal cerebrospinal fluid, and to analyze the feasibility of cerebrospinal fluid for culturing olfactory ensheathing cells used for transplantation. DESIGN, TIME AND SETTING： A completely randomized, block design study was performed at the cell Laboratory, Wuxi Third People＇s Hospital, and Jiangsu Institute of Parasitic Diseases, China, in August 2008. MATERIALS： Dulbecco＇s modified Eagle＇s medium/F12 （DMEM/F12） and fetal bovine serum （Gibco BRL, USA）, mouse anti-rat P75 monoclonal antibody and rabbit anti-glial fibrillary acidic protein polyclonal anti body （＇Santa Cruz Biotechnology, USA）, mouse anti-rat myelin basic protein monoclonal antibody （Cymbus, UK）, mouse anti-rat microtubule-associated protein-2 monoclonal antibody （Transduction Laboratories, USA）, FITC conjugated rabbit anti-mouse monoclonal antibody （Boster, China）, TRITC conjugated goat anti-rabbit monoclonal antibody （Sigma, USA） were used. METHODS： Nasal mucosa olfactory ensheathing cells were separately incubated in DMEM/F12, cerebrospinal fluid, and changing DMEM/F12 into cerebrospinal fluid. Adult female Sprague Dawley rat models of spinal hemisection were established. Nerve injury was repaired by transplantation of nasal mucosa olfactory ensheathing cells cultured in cerebrospinal fluid or DMEM/F12. MAIN OUTCOME MEASURES： The proliferative ability of olfactory ensheathing cells cultured in cerebrospinal fluid was determined by a 3-（4, 5-dimethylthiazol-2-yl）-2, 5-diphenyl tetrazolium bromide assay. The morphology and purity of olfactory ensheathing cells were detected using immunohistochemistry. Animal behavior was evaluated by the Basso, Beattie and Bresnahan locomotor rating scale. Morphological repair was assessed by a horseradish peroxidase-tetra

关键词： cerebrospinal fluid olfactory ensheathing cells in vitro culture cell transplantation

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Stem cell transplantation for treating spinal cord injury A literature comparison between studies of stem cells obtained from various sources

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Neural Regeneration Research 2012年第16期7卷 1256-1263页

作者： Liangbi Xiang Yu Chen Department of Orthopedics General Hospital of Shenyang Military Area Command of Chinese PLAShenyang 110016Liaoning ProvinceChina

OBJECTIVE： To identify global research trends of stem cell transplantation for treating spinal cord injury using a bibliometric analysis of the Web of Science. DATA RETRIEVAL： We performed a bibliometric analysis of data retrievals for stem cell transplantation for treating spinal cord injury from 2002 to 2011 using the Web of Science. SELECTION CRITERIA： Inclusion criteria：（a） peer-reviewed articles on stem cell transplantation for treating spinal cord injury that were published and indexed in the Web of Science; （b） type of articles： original research articles, reviews, meeting abstracts, proceedings papers, book chapters, editorial material, and news items; and （c） year of publication： 2002-2011. Exclusion criteria：（a） articles that required manual searching or telephone access; （b） documents that were not published in the public domain; and （c） a number of corrected papers from the total number of articles. MAIN OUTCOME MEASURES：（1） Annual publication output; （2） distribution according to country; （3） distribution according to institution; （4） distribution according to journals; （5） distribution according to funding agencies; and （6） top cited articles over the last 10 years. RESULTS： Bone marrow mesenchymal stem cells and embryonic stem cells have been widely used for treating spinal cord injury. In total, 191 studies of bone marrow mesenchymal stem cell transplantation and 236 studies of embryonic stem cell transplantation for treating spinal cord injury appeared in the Web of Science from 2002 to 2011, and almost half of which were derived from American or Japanese authors and institutes. The number of studies of stem cell transplantation for treating spinal cord injury has gradually increased over the past 10 years. Most papers on stem cell transplantation for treating spinal cord injury appeared in journals with a particular focus on stem cell research, such as Stem cells and cell transplantation. Although umbilical cord blood stem cells and adipose-deri

关键词： bone marrow mesenchymal stem cells embryonic stem cells umbilical cord blood-derivedmesenchymal stem cells adipose-derived mesenchymal stem cells cell transplantation spinal cordinjury bibliometric Web of Science neural regeneration

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Adipose-derived mesenchymal stem cell transplantation promotes adult neurogenesis in the brains of Alzheimer's disease mice

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Neural Regeneration Research 2014年第8期9卷 798-805页

作者： Yufang Yan Tuo Ma Kai Gong Qiang Ao Xiufang Zhang Yao Gong State Key Laboratory of Biomembrane and Membrane Biotechnology School of Life SciencesTsinghua University Institute of Neurological Disorders Yuquan HospitalTsinghua University

In the present study, we transplanted adipose-derived mesenchymal stem cells into the hippo-campi of APP/PS1 transgenic Alzheimer＇s disease model mice. Immunofluorescence staining revealed that the number of newly generated （BrdU＋） cells in the subgranular zone of the dentate gyrus in the hippocampus was signiifcantly higher in Alzheimer＇s disease mice after adipose-de-rived mesenchymal stem cell transplantation, and there was also a significant increase in the number of BrdU＋/DCX＋neuroblasts in these animals. Adipose-derived mesenchymal stem cell transplantation enhanced neurogenic activity in the subventricular zone as well. Furthermore, adipose-derived mesenchymal stem cell transplantation reduced oxidative stress and alleviated cognitive impairment in the mice. Based on these ifndings, we propose that adipose-derived mes-enchymal stem cell transplantation enhances endogenous neurogenesis in both the subgranular and subventricular zones in APP/PS1 transgenic Alzheimer＇s disease mice, thereby facilitating functional recovery.

关键词： nerve regeneration stem cells Alzheimer's disease adipose-derived mesenchymal stemcells cell transplantation cognitive impairment oxidative stress neurogenesis 863 Program neuralregeneration

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Propofol injection combined with bone marrow mesenchymal stem cell transplantation better improves electrophysiological function in the hindlimb of rats with spinal cord injury than monotherapy

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Neural Regeneration Research 2015年第4期10卷 636-643页

作者： Yue-xin Wang Jing-jing Sun Mei Zhang Xiao-hua Hou Jun Hong Ya-jing Zhou Zhi-yong Zhang First Department of Orthopedics Tangshan Worker’s Hospital Affiliated to Hebei Medical University Department of Neurology Tangshan Union Medical College Hospital Operating Room Tangshan Worker’s Hospital Affiliated to Hebei Medical University Department of Neurosurgery Tangshan Worker’s Hospital Affiliated to Hebei Medical University Department of Anesthesiology Xingtai People’s Hospital Affiliated to Hebei Medical University

The repair effects of bone marrow mesenchymal stem cell transplantation on nervous system damage are not satisfactory. Propofol has been shown to protect against spinal cord injury. Therefore, this study sought to explore the therapeutic effects of their combination on spinal cord injury. Rat models of spinal cord injury were established using the weight drop method. Rats were subjected to bone marrow mesenchymal stem cell transplantationvia tail vein injection and/or propofol injectionvia tail vein using an infusion pump. Four weeks after cell transplan-tation and/or propofol treatment, the cavity within the spinal cord was reduced. The numbers of PKH-26-positive cells and horseradish peroxidase-positive nerve ifbers apparently increased in the spinal cord. Latencies of somatosensory evoked potentials and motor evoked potentials in the hindlimb were noticeably shortened, amplitude was increased and hindlimb motor function was obviously improved. Moreover, the combined effects were better than cell transplantation or propofol injection alone. The above data suggest that the combination of propofol injection and bone marrow mesenchymal stem cell transplantation can effectively improve hindlimb electro-physiological function, promote the recovery of motor funtion, and play a neuroprotective role in spinal cord injury in rats.

关键词： nerve regeneration bone marrow mesenchymal stem cells propofol spinal cord injury cell transplantation electrophysiology motor function stem cells neuroprotection neural regeneration

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Macrophage depletion and Schwann cell transplantation reduce cyst size after rat contusive spinal cord injury

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Neural Regeneration Research 2018年第4期13卷 684-691页

作者： Yee-Shuan Lee Lucy H.Funk Jae K.Lee Mary Bartlett Bunge The Miami Project to Cure Paralysis University of Miami Miller School of Medicine Department of Neurological Surgery University of Miami Miller School of Medicine Department of cell Biology University of Miami Miller School of Medicine

Schwann cell transplantation is a promising therapy for the treatment of spinal cord injury（SCI） and is currently in clinical *** our continuing efforts to improve Schwann cell transplantation strategies,we sought to determine the combined effects of Schwann cell transplantation with macrophage *** macrophages are major inflammatory contributors to the acute spinal cord injury,and are the major phagocytic cells,we hypothesized that transplanting Schwann cells after macrophage depletion will improve cell survival and integration with host tissue after *** test this hypothesis,rat models of contusive SCI at thoracic level 8 were randomly subjected to macrophage depletion or *** rat subjected to macrophage depletion,liposomes filled with clodronate were intraperitoneally injected at 1,3,6,11,and 18 days post *** not subjected to macrophage depletion were intraperitoneally injected with liposomes filled with phosphate buffered *** cells were transplanted 1 week post injury in all *** dextran amine（BDA） was injected at thoracic level 5 to evalute axon *** Basso,Beattie,and Bresnahan locomotor test,Gridwalk test,and sensory test using von Frey filaments were performed to assess functional *** was used to detect glial fibrillary acidic protein,neurofilament,and green fluorescent protein（GFP）,and also to visulize BDA-labelled *** GFP labeled Schwann cell and cyst and lesion volumes were quantified using stained *** numbers of BDA-positive axons were also *** 8 weeks after Schwann cell transplantation,there was a significant reduction in cyst and lesion volumes in the combined treatment group compared to Schwann cell transplantation *** changes were not associated,however,with improved Schwann cell survival,axon growth,or locomotor *** combining Schwann cell transplantation with macrophage depletion does improve histopathology of the in

关键词： Schwann cell Iiposome clodrosome clodronate axon regeneration Schwann cell survival neuroprotection glial scar cyst cell transplantation

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Edaravone combined with Schwann cell transplantation may repair spinal cord injury in rats

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Neural Regeneration Research 2015年第2期10卷 230-236页

作者： Shu-quan Zhang Min-fei Wu Zhe Piao Jin Yao Ji-hai Li Xin-gang Wang Jun Liu Department of Orthopedics Tianjin Nankai Hospital Department of Spine Surgery Orthopedic Hospital Second Hospital Clinical Hospital Jilin University Department of Burns and Plastic Surgery China-Japan Union Hospital Jilin University Department of Hand and Foot Surgery and Reparative & Reconstructive Surgery Center Orthopedic Hospital Second Hospital Clinical Hospital Jilin University

Edaravone has been shown to delay neuronal apoptosis, thereby improving nerve function and the microenvironment after spinal cord injury. Edaravone can provide a favorable environment for theAa：eatment of spinal cord injury using Schwann cell transplantation. This study used rat models of complete spinal cord transection at T9. Six hours later, Schwann cells were transplanted in the head and tail ends of the injury site. Simultaneously, edaravone was injected through the caudal vein. Eight weeks later, the PKH-26-1abeled Schwann cells had survived and migrated to the center of the spinal cord injury region in rats after combined treatment with edaravone and Schwann cells. Moreover, the number of PKH-26-1abeled Schwann cells in the rat spinal cord was more than that in rats undergoing Schwann cell transplantation alone or rats without any treatment. Horseradish peroxidase retrograde tracing revealed that the number of horserad- ish peroxidase-positive nerve fibers was greater in rats treated with edaravone combined with Schwann cells than in rats with Schwann cell transplantation alone. The results demonstrated that lower extremity motor function and neurophysiological function were better in rats treated with edaravone and Schwann cells than in rats with Schwann cell transplantation only. These data confirmed that Schwann cell transplantation combined with edaravone injection promoted the regeneration of nerve fibers of rats with spinal cord injury and improved neurological function.

关键词： nerve regeneration spinal cord injury Schwann cells cell transplantation edaravone motor function electrophysiological function neural regeneration

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Stem cell transplantation for treating Parkinson's disease Literature analysis based on the Web of Science

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Neural Regeneration Research 2012年第16期7卷 1272-1279页

作者： Runhui Li Department of Neurology Fengtian Hospital of Shenyang Medical College Shenyang 110024 Liaoning Province China

OBJECTIVE： To identify global research trends of stem cell transplantation for treating Parkinson＇s disease using a bibliometric analysis of the Web of Science. DATA RETRIEVAL： We performed a bibliometric analysis of data retrievals for stem cell transplantation for treating Parkinson＇s disease from 2002 to 2011 using the Web of Science. SELECTION CRITERIA： Inclusion criteria：（a） peer-reviewed articles on stem cell transplantation for treating Parkinson＇s disease which were published and indexed in the Web of Science; （b） type of articles： original research articles, reviews, meeting abstracts, proceedings papers, book chapters, editorial material and news items; （c） year of publication： 2002-2011. Exclusion criteria：（a） articles that required manual searching or telephone access; （b） we excluded documents that were not published in the public domain; （c） we excluded a number of corrected papers from the total number of articles. MAIN OUTCOME MEASURES：（1） Type of literature; （2） annual publication output; （3） distribution according to journals; （4） distribution according to subject areas; （5） distribution according to country; （6） distribution according to institution; （7） comparison of countries that published the most papers on stem cell transplantation from different cell sources for treating Parkinson＇s disease; （8） comparison of institutions that published the most papers on stem cell transplantation from different cell sources for treating Parkinson＇s disease in the Web of Science from 2002 to 2011; （9） comparison of studies on stem cell transplantation from different cell sources for treating Parkinson＇s disease RESULTS： In total, 1 062 studies on stem cell transplantation for treating Parkinson＇s disease appeared in the Web of Science from 2002 to 2011, almost one third of which were from American authors and institutes. The number of studies on stem cell transplantation for treating Parkinson＇s disease had gradually increased over the past 10 years. Pape

关键词： Parkinson's disease neural stem cells embryonic stem cells bone marrow mesenchymal stemcells cell transplantation bibliometric analysis Web of Science neural regeneration

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Chitosan-collagen porous scaffold and bone marrow mesenchymal stem cell transplantation for ischemic stroke

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Neural Regeneration Research 2015年第9期10卷 1421-1426页

作者： Feng Yan Wei Yue Yue-lin Zhang Guo-chao Mao Ke Gao Zhen-xing Zuo Ya-jing Zhang Hui Lu Department of Neurosurgery the Third Affiliated Hospital of Xi’an Jiaotong University Shaanxi Provincial People’s Hospital Department of Neurosurgery the Fourth People’s Hospital of Shaanxi Department of Neurology Tianjin Huanhu Hospital Department of Neurosurgery the First Affiliated Hospital of Xi’an Jiaotong University

In this study, we successfully constructed a composite of bone marrow mesenchymal stem cells and a chitosan-collagen scaffold in vitro, transplanted either the composite or bone marrow mesenchymal stem cells alone into the ischemic area in animal models, and compared their effects. At 14 days after co-transplantation of bone marrow mesenchymal stem cells and the hi- tosan-collagen scaffold, neurological function recovered noticeably. Vascular endothelial growth factor expression and nestin-labeled neural precursor cells were detected in the iscbemic area, surrounding tissue, hippocampal dentate gyrus and subventricular zone. Simultaneously, a high level of expression of glial fibrillary acidic protein and a low level of expression of neuron-spe- cific enolase were visible in BrdU-labeled bone marrow mesenchymal stem cells. These findings suggest that transplantation of a composite of bone marrow mesenchymal stem cells and a chi- tosan-collagen scaffold has a neuroprotective effect following ischemic stroke.

关键词： nerve regeneration ischemic stroke chitosan-collagen scaffold bone marrow mesenchymalstem cells cell transplantation cell differentiation neurological function neural regeneration

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Application of magnetic resonance imaging for monitoring stem cell transplantation for the treatment of cerebral ischemia

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Neural Regeneration Research 2012年第16期7卷 1264-1271页

作者： Xianglin Zhang Gang Wang Furen Dong Zhiming Wang Department of Radiology First Affiliated Hospital of Liaoning Medical University Jinzhou 121001 Liaoning Province China Third Affiliated Hospital of Liaoning Medical University Jinzhou 121001 Liaoning Province China

OBJECTIVE： To identify global research trends in the application of MRI for monitoring stem cell transplantation using a bibliometric analysis of Web of Science. DATA RETRIEVAL： We performed a bibliometric analysis of studies relating to the application of MRI for detecting stem cell transplantation for the treatment of cerebral ischemia using papers in Web of Science published from 2002 to 2011. SELECTION CRITERIA： The inclusion criteria were：（a） peer-reviewed articles on the application of MRI for detecting transplanted stem cells published and indexed in Web of Science; （b） year of publication between 2002 and 2011. Exclusion criteria were：（a） articles that required manual searching or telephone access; （b） some corrected papers. MAIN OUTCOME MEASURES：（1） Annual publication output; （2） distribution according to journals; （3） distribution according to institution; （4） distribution according to country; （5） top cited authors over the last 10 years. RESULTS： A total of 1 498 studies related to the application of MRI for monitoring stem cell transplantation appeared in Web of Science from 2002 to 2011, almost half of which were derived from American authors and institutes. The number of studies on the application of MRI for detecting stem cell transplantation has gradually increased over the past 10 years. Most papers on this topic appeared in Magnetic Resonance in Medicine. CONCLUSION： This analysis suggests that few experimental studies have been investigated the use of MRI for tracking SPIO-labeled human umbilical cord blood-derived mesenchymal stem cells during the treatment of cerebral ischemia.

关键词： neural stem cells embryonic stem cells bone marrow mesenchymal stem cells cell transplantation cerebral ischemia Web of Science neural regeneration

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