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An ethanolic extract of Bailian(Radix Ampelopsis Japonicae):demonstration of colorectal cancer treatment efficacy via inhibition of β-catenin signaling in vitro

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Journal of Traditional Chinese Medicine 2019年第3期39卷 339-345页

作者： Su Tao Wang Xinning Li Chunyu Bai Jingxuan Chi-Yan Cheng Fu Xiuqiong Li Ting Yu Zhiling Consun Chinese Medicines Research Centre for Renal Diseases School of Chinese Medicine Hong Kong Baptist University Kowloon Tong Hong Kong 999077 China HKBU Shenzhen Research Institute and Continuing Education Shenzhen 518001 China Centre for Cancer and Inflammation Research School of Chinese Medicine Hong Kong Baptist University Hong Kong999077 China

OBJECTIVE:To investigate the underlying mechanism of Bailian (Radix Ampelopsis Japonicae,BL) extract action on colorectal cancer (CRC).METHODS:We explored the involvement of β-catenin signaling on the anti-CRC effects of an BL ethanolic extract (BLE) in cell models by using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay,immunofluorescent staining,luciferase assay,Western blot analysis and real-time quantitative polymerase chain reaction ***-CRC compounds were quantified by high performance liquid ***:The contents of gallic acid,catechin,and epicatechin in the BLE were 0.23,1.25,and 0.18 g/kg,***-mediated cytotoxic and apoptotic effects were accompanied by lowered β-catenin/Tcf transcriptional activity,reduced β-catenin nuclear localization,and downregulated protein and mRNA levels of both β-catenin and molecules regulated by β-***:The mechanism underpinning the anti-CRC effects of BLE may involve inhibition of β-catenin *** studies are necessary to establish the role of β-catenin signaling in the action of BLE-mediated anti-CRC effects.

关键词： Bailian (Radix Ampelopsis Japonicae) Colorectal neoplasms Beta catenin Cytotoxins Apoptosis

Effects of Retinoic Acid on the β-catenin/TCF Pathway in Cultured Porcine Tracheobronchial Epithelial Cells

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Journal of Huazhong University of Science and Technology(Medical Sciences) 2004年第5期24卷 421-423,432页

作者：李媛吴人亮王曦陈文书 Department of Pathology School of Basic Medical Sciences Tongji Medical College Huazhong University of Science and Technology Wuhan 430030

The effects of retinoic acid on the β-catenin/TCF pathway in cultured porcine tracheobronchial epithelial cells (TBEC) were investigated. After TBEC were treated with retinoic acid at various concentrations, mRNA and protein changes of β-catenin in cytoplasm, nucleus and whole cell of the TBEC were observed by immunocytochemical stain, RT-PCR and Western blotting. And the changes of the target gene cyclinD1 of β-catenin/TCF pathway were also observed. It was found that there was no significant difference in β-cat mRNA level after retinoic acid treatment. However, the expression of β-catenin in the whole cell and cytoplasm was elevated with the increase of retinoic acid concentration (Pcatenin and target gene cyclinD1 of β-catenin/TCF pathway was decreased (Pcatenin level of the whole cell protein and decrease nuclear β-catenin, downregulating β-cat/TCF signaling activity and reducing target gene cyclinD1 protein level. As a result, retinoic acid can downregulate β-catenin/TCF pathway in porcine tracheobronchial epithelial cell, suggesting that retinoic acid can inhibit the proliferation and accelerate differentiation of tracheobronchial epithelial cells.

关键词： catenin retinoic acid cyclinD1 tracheobronchial epithelial cells

EXPRESSION OF E-CADHERIN/catenin COMPLEX IN BREAST CANCER

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Chinese Journal of Cancer Research 2006年第4期18卷 299-305页

作者：刘臻崔东旭刘宝林张小薄马文锋张强 The First Department of General Surgery the Second Affiliated Hospital China Medical University the Third Department of General Surgery the First Affiliated Hospital China Medical University

Objective： To detect the expressions of E-cadherin, α-catenin and β-catenin and analyze the relationship between Ecadherin-catenin adhesion complex and clinicopathological features in breast cancer. Methods： The expressions of E-cadherin, α-cadherin and β-catenin in specimens of 54 breast cancer, 21 normal breast tissues around tumor, 15 breast hyperplasia of usual type and 15 breast atypical hyperplasia were detected by immunohistochemical method. Results： In 21 normal breast tissues, E-cadherin and α-catenin were expressed on cell membrane of ductal and acinic cells, showing cellular contour and border among cells. The staining character of the three proteins in breast hyperplasia of usual type was the same as that in normal breast tissue. In breast atypical hyperplasia, the abnormal expression rates of E-cadherin, α-catenin and β-catenin were 6.7%, 13.3% and 26.7%, respectively. The total abnormal expression rate of E-cadherin-catenin complex was 33.3%. In breast cancer, the abnormal expression rates of E-cadherin, α＋catenin and β-catenin were 51.9%, 63.0% and 61.1%, respectively. The total abnormal expression rate of E-cadherin-catenin complex was 88.9%. Abnormal expression of E-cadherin and α-catenin were significantly correlated with histological grade. Abnormal expressions of α-catenin and β-catenin were significantly correlated with TNM staging, axillary lymph nodes metastasis and postoperative distant metastasis. Abnormal expression of E-cadherin-catenin complex was correlated with TNM staging, histological grade and axillary lymph nodes. Abnormal expression of β-catenin was negatively correlated with expression of HER-2. COX multiple factor analysis showed that E-cadherin or α-catenin or β-catenin was not independent prognostic indicator. Conclusion： Abnormal expressions of E-cadherin, α-catenin and β-catenin frequently occur in breast cancer. Abnormal expression of E-cadherin-catenin complex is correlated with differentiation disturbance and metastasis. C

关键词： Breast neoplasms E-cadherin catenin

Relationship between expressions of E-cadherin and alpha-catenin and biological behaviors of human pancreatic cancer

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Hepatobiliary & Pancreatic Diseases International 2003年第2期2卷 471-477页

作者： Yu-Jun Li Yun-Xiao Meng Xiang-Rui Ji the Department of Pathology. Affiliated Hospital of Medical College, Qingdao University, Qingdao 266003, China Department of Pathology Affiliated Hospital of Medical College Qingdao University Qingdao 266003 China. yujun-li66@***

OBJECTIVES: To investigate the expressions of E-eadherin and alpha-catenin in pancreatic carcinoma and their relationship with biological behaviors, and clarify the mechanism of invasion and metastasis of pancreatic cancer. METHODS: The expressions of E-eadherin and alpha-catenin was examined in 47 patients with infiltrative ductal adenocarcinoma of the pancreas and 12 specimens of normal pancreatic tissues by immunohistochemical technique (PicTure^(TM) two-step method). Proliferation cell nuclear antigen (PCNA) was tested as an index of the proliferation degree of pancreatic cancer cells. RESULTS: The immunoreactivity of E-cadherin and alpha-catenin was expressed by normal ductal and acinar cells with strong membranous staining at the intercellular border in 12 specimens of normal pancreatic tissues. The abnormal rate of E-cadherin expression in pancreatic cancer was 53.2% (25/47), and it was significantly related to differentiation, high proliferation degree and lymph node and liver metastases (Pcatenin. There was a good correlation among alpha-catenin expression, histological grade, and lymph node and liver metastases (Pcatenin, tumor size, invasion, and 1-year survival rate of patients ( P>0.05, all). There was a positive relationship between the expressions of E-cadherin and alpha-catenin in the 47 patients with pancreatic cancer (Pcatenin genes and abnormal expression of proteins, which significantly correlate with tumorigenesis, proliferation, differentiation, and lymph node or liver metastasis of pancreatic cancer.

关键词： pancreatic neoplasms cadherin catenin metastases prognosis immunohistochemistry

贲门癌中α-catenin表达及与临床病理的关系

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中华消化杂志 2006年第9期26卷 621-622页

作者：张雷王鹏穆秋菊张润起刘钦亮山东省泰安市中心医院胸外科 271000 中国煤矿工人泰山疗养院

食管癌中α—catenin（α-cat）表达减少与肿瘤低分化、侵袭性和淋巴结转移呈正相关，本研究应用免疫组化法检测62例贲门癌术后标本中α-cat表达情况，以探讨其与贲门癌生物学行为的关系。

关键词： catenin 贲门癌临床病理免疫组化法检测癌生物学行为淋巴结转移表达减少表达情况

Axin过表达下调β-catenin和TCF-4表达并抑制肺癌BE1细胞的增殖和侵袭

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中国肺癌杂志 2009年第4期12卷 277-282页

作者：刘树立徐洪涛杨连赫李庆昌韦强王恩华中国医科大学基础医学院病胛学教研室中国医科大学附属第一医院病理科沈阳110001

背景与目的Axin是Wnt通路的重要负性调节因子,其低表达和β-catenin、TCF-4的高表达与多种肿瘤有关。本研究旨在探讨axin在肺癌细胞中的表达与β-catenin和TCF-4的关系,及其对肺癌细胞增殖、凋亡和侵袭能力的影响。方法将axin基因转染入... 详细信息

背景与目的Axin是Wnt通路的重要负性调节因子,其低表达和β-catenin、TCF-4的高表达与多种肿瘤有关。本研究旨在探讨axin在肺癌细胞中的表达与β-catenin和TCF-4的关系,及其对肺癌细胞增殖、凋亡和侵袭能力的影响。方法将axin基因转染入axin低表达的肺癌BE1细胞系,应用免疫荧光方法观察转染组和对照组细胞中axin的表达对β-catenin和TCF-4的影响;采用RT-PCR方法检测axin和β-catenin、TCF-4mRNA表达的变化;采用流式细胞术、MTT和Transwell检测肺癌细胞凋亡、增殖和侵袭能力的变化。结果肺癌BE1细胞转染axin基因后(BE1-axin),axin的mRNA和蛋白水平均明显增加,β-catenin蛋白表达明显减少,TCF-4的蛋白和mRNA表达均明显下降。同时,BE1-axin细胞的凋亡率增加,增殖和侵袭能力明显下降。结论Axin过表达能下调β-catenin的蛋白表达和TCF-4的转录,抑制肺癌细胞的增殖和侵袭能力,并诱导肺癌细胞凋亡。

关键词： Axin Beta catenin TCF-4 肺肿瘤增殖

Wnt/β-catenin信号通路在骨重建中的作用研究进展

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中国康复医学杂志 2015年第5期30卷 513-516页

作者：周君何成奇南华大学附属第一医院康复科湖南衡阳421001 四川大学华西医院康复科康复医学四川省重点实验室

骨重建包括骨吸收及其耦联的骨形成两方面,在正常情况下,成骨细胞介导的骨形成和破骨细胞介导的骨吸收维持动态平衡。Wnt/β-catenin信号通路在骨重建过程中起到重要的作用,与成骨细胞分化、增殖和骨形成密切相关,并通过不同途径影响破... 详细信息

骨重建包括骨吸收及其耦联的骨形成两方面,在正常情况下,成骨细胞介导的骨形成和破骨细胞介导的骨吸收维持动态平衡。Wnt/β-catenin信号通路在骨重建过程中起到重要的作用,与成骨细胞分化、增殖和骨形成密切相关,并通过不同途径影响破骨细胞及骨细胞的功能,现就Wnt/β-catenin信号通路在骨重建过程中的作用作一综述。

关键词：信号通路 Wnt 骨重建 catenin 破骨细胞细胞介导骨吸收去卵巢大鼠脂肪细胞分化相关因子

胰腺癌Wnt/β-catenin信号通路的研究进展

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广东医学 2014年第21期35卷 3445-3447页

作者：黄祯肖卫东南昌大学第一附属医院普外二科南昌330006

胰腺癌是一种恶性程度极高的消化道肿瘤,病死率在肿瘤中居第4位,由于胰腺解剖位置的特殊性,大多数胰腺癌确诊时已到晚期,手术切除率低,且对药物治疗不敏感,预后极差,5年生存率不足5%[1]。Wnt/β-catenin信号通路在胚胎发育过程中起着重... 详细信息

胰腺癌是一种恶性程度极高的消化道肿瘤,病死率在肿瘤中居第4位,由于胰腺解剖位置的特殊性,大多数胰腺癌确诊时已到晚期,手术切除率低,且对药物治疗不敏感,预后极差,5年生存率不足5%[1]。Wnt/β-catenin信号通路在胚胎发育过程中起着重要作用,但在正常成年体内该通路异常激活可导致肿瘤的发生。已证实Wnt/β-catenin信号通路与胰腺癌的发生发展有着密切的关系,Wnt/β-catenin信号通路激活可促进胰腺癌肿瘤细胞的增殖、侵袭、转移及抑制肿瘤细胞的凋亡。

关键词： Wnt 信号通路 catenin 消化道肿瘤作用机制胚胎发育基因表达正常胰腺组织药物治疗 cadherin

Wnt/β-catenin信号通路与抗癌治疗新靶点

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中国临床医学 2007年第1期14卷 118-121页

作者：陈劲松梁庆模南华大学附属南华医院湖南衡阳421002

晚期恶性肿瘤治疗效果较差，近几10年来其死亡率无明显改善，这是当今医学界面临的一大难题。手术治疗、化疗、放疗是恶性肿瘤的三大常规治疗手段，其中化疗在晚期恶性肿瘤的治疗中占有重要地位。但大多数化疗药物在抑制肿瘤细胞生长的... 详细信息

晚期恶性肿瘤治疗效果较差，近几10年来其死亡率无明显改善，这是当今医学界面临的一大难题。手术治疗、化疗、放疗是恶性肿瘤的三大常规治疗手段，其中化疗在晚期恶性肿瘤的治疗中占有重要地位。但大多数化疗药物在抑制肿瘤细胞生长的同时也可非选择性抑制正常增殖活跃细胞的生长而引起严重的毒副反应；还可导致化疗相关的第二种癌；因而其应用受到了限制。故急需开发针对新靶点的选择性强的高效低毒抗癌药物。Wnt／β—catenin信号传导通路与人类许多肿瘤的发生发展密切相关，尤其在大肠癌的发生发展中起决定性作用。近年发现大量的抗癌治疗是以Wnt／β—catenin信号通路为靶点的。

关键词： catenin 抗癌治疗信号通路新靶点 Wnt 抑制肿瘤细胞生长晚期恶性肿瘤化疗药物