Effects of Retinoic Acid on the β-catenin/TCF Pathway in Cultured Porcine Tracheobronchial Epithelial Cells

作     者：李媛 吴人亮 王曦 陈文书 

作者机构：Department of Pathology School of Basic Medical Sciences Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 

出 版 物：《Journal of Huazhong University of Science and Technology(Medical Sciences)》 (华中科技大学学报（医学英德文版）)

年 卷 期：2004年第24卷第5期

页      面：421-423,432页

核心收录：

学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 100706[医学-药理学] 1001[医学-基础医学(可授医学、理学学位)] 0805[工学-材料科学与工程（可授工学、理学学位）] 0703[理学-化学] 0836[工学-生物工程] 100602[医学-中西医结合临床] 10[医学] 

基　　金：ThisprojectwassupportedbyagrantfromNationalNaturalSciencesFoundationofChinaforyouthscientist (No .30 2 0 0 1 1 5 ) 

主　　题：catenin retinoic acid cyclinD1 tracheobronchial epithelial cells 

摘      要：The effects of retinoic acid on the β-catenin/TCF pathway in cultured porcine tracheobronchial epithelial cells (TBEC) were investigated. After TBEC were treated with retinoic acid at various concentrations, mRNA and protein changes of β-catenin in cytoplasm, nucleus and whole cell of the TBEC were observed by immunocytochemical stain, RT-PCR and Western blotting. And the changes of the target gene cyclinD1 of β-catenin/TCF pathway were also observed. It was found that there was no significant difference in β-cat mRNA level after retinoic acid treatment. However, the expression of β-catenin in the whole cell and cytoplasm was elevated with the increase of retinoic acid concentration (P0.01). The nuclear protein β-catenin and target gene cyclinD1 of β-catenin/TCF pathway was decreased (P0.05). It was indicated that retinoic acid could increase β-catenin level of the whole cell protein and decrease nuclear β-catenin, downregulating β-cat/TCF signaling activity and reducing target gene cyclinD1 protein level. As a result, retinoic acid can downregulate β-catenin/TCF pathway in porcine tracheobronchial epithelial cell, suggesting that retinoic acid can inhibit the proliferation and accelerate differentiation of tracheobronchial epithelial cells.