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Biomedical and Environmental Sciences 2010年第2期23卷 151-157页

作者： YI-XIONG LEI MIN WANG LIAN WEI XI LU HUA-ZHAO LIN Department of Preventive Medicine Guangzhou Medical University Guangzhou 510182 Guangdong China Institute for Chemical Carcinogenesis Guangzhou Medical University Guangzhou 510182 Guangdong China

Objective To study the alternative expression and sequence of human elongation factor-1δ （human EF-1δ p31） during malignant transformation of human bronchial epithelial cells induced by cadmium chloride （CdCl2） and its possible mechanism. Methods Total RNA was isolated at different stages of transformed human bronchial epithelial cells （16HBE） induced by CdCl2 at a concentration of 5.0 μM. Special primers and probe for human EF-1δ p31 were designed and expression of human EF-18 mRNA from different cell lines was detected with fluorescent quantitative PCR technique. EF-18 cDNA from different cell lines was purified and cloned into pMD 18-T vector followed by confirming and sequencing analysis. Results The expressions of human EF-1δ p31 at different stages of 16HBE cells transformed by CdCl2 was elevated （P〈0.01 or P〈0.05）. Compared with their corresponding non-transformed ceils, the overexpression level of EF-15 p31 was averagely increased 2.9 folds in Cd-pretransformed cells, 4.3 folds in Cd-transformed ceils and 7.2 folds in Cd-tumorigenic cells. No change was found in the sequence of overexpressed EF-1δ p31 at different stages of 16HBE cells transformed by CdCl2. Conclusion Overexpression of human EF-1δ p31 is positively correlated with malignant transformation of 16HBE cells induced by CdCl2, but is not correlated with DNA mutations.

关键词： Human elongation factor-1δ cadmium chloride Human bronchial epithelial cells Cell transformation Sequencing analysis

Malignant Transformation and Abnormal Expression of Eukaryotic Initiation Factor in Bronchial Epithelial Cells Induced by cadmium chloride

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Biomedical and Environmental Sciences 2008年第4期21卷 332-338页

作者： YI-XIONG LEI LIAN WEI MIN WANG GEN-RONG WU MIN LI Department of Preventive Medicine Guangzhou Medical College Guangzhou 510182 Guangdong China

Objective To analyze the relationship between malignant transformation and abnormal expression of eukaryotic initiation factor 3 （eIF3 p36） in human bronchial epithelial （16HBE） cells induced by cadmium chloride （CdCl2）. Methods 16HBE cells were treated several times with different concentrations of CdCl2. Tumorigenic potential of transformed cells was identified by assays for anchorage-independent growth in soft agar and for tumorigenicity in nude mice after the 35th passage. Total RNA was isolated from 16HBE cells induced by CdC12, including non-transformed, Cd-transformed, and Cd-tumorigenic cell lines. Special primers for eIF3 p36 were designed and the expression of eIF3 mRNA in different cell lines was detected with fluorescent quantitative-polymerase chain reaction technique （FQ-PCR）. Results The 35th passage of 16HBE cells transformed by CdCl2 exhibited overlapping growth. Compared with the non-transformed cells, colonies of transformed cell lines in soft agar showed statistically significant increases and dose-dependent effects （P〈0.01）. All Cd-induced transformed cell lines formed rumors in nude mice within 2 weeks of inoculation, but none of the mice injected with non-transformed cells showed tumors even after 3 weeks. All tumors were pathologically identified as poorly differentiated squamous cell carcinoma. The eIF3 p36 genes in different stages of 16HBE cells transformed by CdCl2 were elevated as compared with the non-transformed control （P〈0.01）, and the eIF3 expression increased with the degree of cell malignancy. Conclusion CdCl2 is capable of inducing morphological transformation in 16HBE cells and transformed cells are potentially tumorigenic. Over-expression of eIF3 p36 is positively correlated with malignant transformation of 16HBE cells induced by CdCl2 and may be one of the molecular mechanisms potentially responsible for carcinogenesis due to Cd.

关键词： Cell transformation Tumorigenicity Eukaryotic initiation factor 3 cadmium chloride Human bronchial epithelial cells

The effect of ASPM gene in the acute damage induced by cadmium chloride for 16HBE cells

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The effect of ASPM gene in the acute damage induced by Cadmi...

The reproductive toxicity of cadmium chloride in male mice

中国毒理学会第七次全国会员代表大会暨中国毒理学会第六次中青年学者科技论坛

作者： Shanshan Z Liang R Piaofan Y Xiaoxuan Y Ping Y Yixiong L Guangzhou Medical University School of Public Health Department of Prevention Medicine School of Public Health Guangzhou Medical University

Objective This studyattempts to investigate the relationships between ASPM role and lung tumor cadmium caused, which might be a new biomarker for diagnosis, treatmentand prognosis of lung *** The expression of ASPM at m RNA and protein level in 16HBE cells under CdCl2 treatment was detedcted by qRT-PCR and immunohistochemical *** low-expression cell lines were constructed by hairpin RNA(shR-NA) for obseving cell changes which were treated with CdCl2. 16HBE cells were treated respectively with control,10μM/L, 20μM/L, 30μM/L and 40μM/L CdCl2 for 24h, and then 16HBE cellswere treated with30μM/L CdCl2 for 12h, 24h, 48h and 72h. The function of ASPM gene was detected by MTT,comet assay, cell flow technology and Cytochrome c apoptosis assay. Subsequently, these datas were statistically *** With the dose and time increase of CdCl2, the expression of ASPM upregulated in 16HBE cells. Compared with 16HBE cells, there was no statistical differences of growth and morphlogy in cells proliferation of ASPM dificiency. Our data, from the Comet assay in detail, indicated that cadmium chloride caused DNA damage in 16HBE cells in a concentration-time dependent manner, yet the seriousness of DNA damage was more in 16HBEshASPM cells. In H2O2-induced oxidative damage, the DNA damage, for down-regulating the expression of ASPM, was also more than control group. In MTT, ASPM gene can be related to decreasing cell viability in the process of cell damage induced by cadmium chloride. With theincrease of concentration and time, the release of cytochrome C increased. In 16HBE-shASPM cell, the release of cytochrome C was higher than 16HBE cells at the identical procedure. It indicated that ASPM gene under-expression could increase apoptosis and release cytochrome *** Abnormal spindle microtubule assembly (ASPM) gene was regarded as a biomarkers for diverse tumors. However, the research has not been particularly obvious in regarded to carcinogenic mecha

关键词： 16HBE cells ASPM cadmium chloride

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Asian Journal of Andrology 2004年第1期6卷 58-58页

作者： Long-JinJin Jun-MingZhang Zhe-FengLou Jie-YingDong DepartmentofBiology WenzhouMedicalCollegeWenzhou325027China

Objective: To study the reproductive toxicity of cadmium chloride in male mice. Methods: Male mice of 4 weeks old were administered cadium chloride at doses of 0.5, 2 or 8 mg·kg-1·day-1 i.p. for 10 days. At day 50, the males were mated with virgin females at 1: 2. The pregnancy rate, the litter size and the body weight of the offspring were recorded; at the same time the development of the testis, the testicular index, the germ cell miosis and the epididymal sperm count, motility and malformed sperm were observed in the intoxicated males. Results: The testicular index was lower in the 2 and 8 mg/kg groups than in the controls and 0.5 mg/kg groups (Pcadmium chloride group was not significantly different from that of the controls. The sperm count, motility and malformed sperm were not significantly different between the 0.5 mg/kg group and the controls. Conclusion: cadmium chloride, at doses of 2 mg/kg or more, reduces the pregnancy rate, which may be related to testis maldevelopment and epididymal azoospermia. Androl

关键词： cadmium chloride mice testis pregnancy

Effects of cadmium on Rat Sperm Motility Evaluated With Computer Assisted Sperm Analysis

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Biomedical and Environmental Sciences 2001年第4期14卷 312-317页

作者： XULI－CHUN WANGSHU－YI KeyLaboratoryforAppliedToxicologyofJiangsuProvince NanjingMedicalUniversityNanjing210029China KeyLaboratoryforAppliedToxicologyofJiangsuProvince NanjingMedicalUniversityNanjing

Objective To study effects of cadmium on rat sperm motility evaluated with computer assisted sperm analysis. Methods Different doses of cadmium chloride (0.2,0.4,0.8mg Cd/kg BW) were administrated ip to adult male Sprague_Dawley rats. Control animals received the same volume of 0.9% NaCl solution. After 7 days, the rats were sacrificed with their testes removed. A part of one testis was used for testicular sperm head counts and daily sperm production observation. The motility of spermatozoa obtained from cauda epididymides using the 'diffusion'method was measured by computer assisted sperm analysis(CASA). Results The sperm head counts and daily sperm production decreased significantly in the high dose group. The motility of spermatozoa in the middle dose group was reduced significantly. No motile sperm was found in the high dose group. The results suggest that germinal epithelium was impaired irreversibly in a short time to produce toxic effects on spermatogenesis at high cadmium doses. Conclusion cadmium may reduce sperm motility at a dose far below the dose affecting sperm production at this time point. The motility of sperm is an early and sensitive endpoint for the assessment of cadmium toxicity on male reproduction.

关键词： Image Processing Computer-Assisted Animals cadmium chloride Dose-Response Relationship Drug Male Rats Rats Sprague-Dawley Research Support Non-U.S. Gov't Sperm Count Sperm Motility Spermatogenesis

Preventive effects of β-cryptoxanthin against cadmium-induced oxidative stress in the rat testis

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Asian Journal of Andrology 2016年第6期18卷 920-924页

作者： Xiao-Ran Liu Yue-Ying Wang Hai-Rui Fan Can-Jie Wu Ashok Kumar Li-Guo Yang Key Laboratory of Agricultural Animal Genetics Breeding and Reproduction Ministry of Education College of Animal Science and Technology Huazhong Agricultural University Wuhan 430070 China State Key Laboratory of Agricultural Microbiology College of Life Science and Technology Huazhong Agricultural University Wuhan 430070 China

β-cryptoxanthin （CRY）, a major carotenoid of potential interest for health, is obtained naturally from orange vegetables and fruits. A few research studies have reported that CRY could decrease oxidative stress and germ cell apoptosis. The purpose of this study was to examine the effects of CRY on acute cadmium chloride （CdCl2）-induced oxidative damage in rat testes. For this study, 24 rats were divided into four groups, one of which serves as a control group that received intraperitoneal （i.p.） injections of corn oil and physiological saline. The other rats were i.p. injected with CRY （10 μg kg^-1） every 8 h, beginning 8 h before CdCI2 （2.0 mg kg^-1） treatment. The pathological and TUNEL findings revealed that CRY ameliorated the Cd-induced testicular histological changes and germ cell apoptosis in the rats, Furthermore, the Cd-induced decrease in the testicular testosterone （T） level was attenuated after CRY administration （P 〈 0.05）. The administration of CRY significantly reversed the Cd-induced increases in the lipid peroxide （LPO） and malondialdehyde （MDA） levels （P 〈 0.01）. The testicular antioxidants superoxide dismutase （SOD）, catalase （CAT） and glutathione （GSH） were decreased by treatment with Cd alone but were restored by CRY co-treatment. These results demonstrated that the application of CRY can enhance the tolerance of rats to Cd-induced oxidative damage and suggest that it has promised as a pharmacological agent to protect against Cd-induced testicular toxicity.

关键词： apoptosis cadmium chloride cryptoxanthin oxidative stress rat testicular damage testosterone

Effects of Cd^(2+) on the nucleolus in root tip cells of Allium cepa

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Journal of Environmental Sciences 1994年第3期6卷 382-386页

作者： Jiang Wusheng Liu Donghua(Department of Biology,Tianjin Normal University,Tianjin 300074,China)Li Maoxue(Department of Biology,Peking University),Beijing 100871,China)

The effects of different concentrations of cadmium chloride ranging from 0.5 to 20 ppm on the nucleolus in root tip cells of Allium cepa were studied using the silver staining technique. The results indicated that after the treatment with Cd￣(2+),different changes in nucleolar morphology appeared. The nucleolar material was extruded from the nucleus into the cytoplasm,and the nucleoli at mitotic metaphase did not disappear. Apparently,cadmium showed a specific effects on the nucleoli in root tip cells of Allium cepa. The possible mechanism behind this phenomenon is also briefly discussed.

关键词： cadmium chloride Allium cepa,nucleolus.

Cellular Oxidative Damage of HEK293T Cells Induced by Combination of CdCl_2 and Nano-TiO_2

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Journal of Huazhong University of Science and Technology(Medical Sciences) 2011年第3期31卷 290-294页

作者：夏彬陈建伟周宜开 Key Laboratory of Environment and Health of Ministry of Environment Department of Occupational and Environmental HealthSchool of Public HealthTongji Medical CollegeHuazhong University of Science and Technology

This study investigated the conjoined cellular oxidative damage of human embryo kidney 293T（HEK293T） cells induced by cadmium chloride（CdCl2） and nanometer titanium dioxide（nano-TiO2）.RT-PCR technique was used to detect the expressions of Heme oxygenase-1（HO-1） and 8-oxoguanine DNA glycosylase（OGG1）.The activities of superoxide dismutase（SOD） and catalase enzyme（CAT） and concentrations of reactive oxygen species（ROS） and maldondialdehyde（MDA） were measured by different *** results showed that CdCl2 and nano-TiO2 at a low concen-tration of 0.75 total toxic unit（TU） exerted an additive effects on HO-1 gene expression,CAT activities and MDA *** the total TU was increased to 1 or 1.25 TU,the interaction was ***,the mixture with high proportion of CdCl2 produced an additive effect on the OGG1 gene expression,and the interaction was changed to be synergetic when the concentration of CdCl2 was lower than or equal to that of *** effects of CdCl2 and nano-TiO2 on cellular oxida-tive damage of HEK293T cells were found as indicated by the changes in the SOD activities and ROS *** was concluded that CdCl2 and nano-TiO2 exerts synergistic effects on the cellular oxidative damage of HEK293T cells,and the sensitivity of these indicators of oxidative damage varies with the proportion of CdCl2 and nano-TiO2 in the mixture.

关键词： cadmium chloride nano-TiO2 oxidative damage HEK293T cells

Combined Effects of Cd and Hg on Liver and Kidney Histology and Function in Wistar Rats

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Journal of Agricultural Chemistry and Environment 2016年第4期5卷 159-169页

作者： Karima Dardouri Samir Haouem Ines Gharbi Badreddine Sriha Zohra Haouas Abdelhamid El Hani Mohamed Hammami Department of Physiology Faculty of Medicines of Monastir University of Monastir Monastir Tunisia Laboratory of Biochemistry Research Laboratory LR12 ES 05 Lab-NAFS “Nutrition-Functional Food & Vascular Health” Faculty of Medicines University of Monastir Monastir Tunisia Laboratory of Biochemistry Research Laboratory LR12 ES 05 Lab-NAFS “Nutrition-Functional Food & Vascular Health”Faculty of Medicines University of Monastir Monastir Tunisia Laboratory of Pathology University Hospital Farhat Hached Sousse Tunisia Laboratory of Histology Cytology and Genetics Faculty of Medicines of Monastir University of Monastir Monastir Tunisia

The present study was performed in order to discern the effects of combined exposure to cadmium and mercury on liver function and histopathological alterations in male adult Wistar rats. In the present investigation, cadmium (100 mg/l) and mercury (25 mg/l) were administered orally for 10 weeks separately or in combination. The rational for studying cadmium and mercury is that both of these metals are encountered frequently in the same contaminated areas. In liver, the activities of serum alanine aminotransferase (ALT) and aspartate amino tranferase (AST) increased significantly in the cadmium (Cd) and mercury (Hg) alone or in combination (Cd + Hg) compared to the control suggesting that both cadmium and mercury cause hepatotoxicity spatially when co-administrated. We noted an increase in serum lactate dehydrogenase (LDH) activity in Cd and combined Cd + Hg treated groups while it decreased in Hg treated group. There was no statistically significant change in the level of total bilirubilin. Serum urea concentration showed a significant increase in the Cd and Hg groups compared to the control group. However an increase in serum creatinine concentration was noted only in the combined treated rats showing that renal insufficiency is more serious in the co-exposed group. Light microscopic examination indicated severe histological changes in the two organs under Cd and mercury influence. Results of the present investigation clearly showed that mercury has profound effects of hepatic handling of cadmium (synergistic effect) as shown by histological and biochemical results. Moreover, we observed a antagonist effect between these two toxic metals on kidney markers such as urea.

关键词： cadmium chloride Mercuric chloride Hepatotoxicity Kidney Damage Rat

Effects on Apoptosis of Spermatogenic Cells and Bax,Bcl-2 Proteins by cadmium

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Effects on Apoptosis of Spermatogenic Cells and Bax,Bcl-2 Pr...