Cellular Oxidative Damage of HEK293T Cells Induced by Combination of CdCl_2 and Nano-TiO_2
Cellular Oxidative Damage of HEK293T Cells Induced by Combination of CdCl_2 and Nano-TiO_2作者机构：Key Laboratory of Environment and Health of Ministry of EnvironmentDepartment of Occupational and Environmental HealthSchool of Public HealthTongji Medical CollegeHuazhong University of Science and Technology
出 版 物：《Journal of Huazhong University of Science and Technology(Medical Sciences)》 (华中科技大学学报（医学英德文版）)
年 卷 期：2011年第31卷第3期
页 面：290-294页
核心收录：
学科分类：100405[医学-卫生毒理学] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 10[医学]
主 题：cadmium chloride nano-TiO2 oxidative damage HEK293T cells
摘 要：This study investigated the conjoined cellular oxidative damage of human embryo kidney 293T（HEK293T） cells induced by cadmium chloride（CdCl2） and nanometer titanium dioxide（nano-TiO2）.RT-PCR technique was used to detect the expressions of Heme oxygenase-1（HO-1） and 8-oxoguanine DNA glycosylase（OGG1）.The activities of superoxide dismutase（SOD） and catalase enzyme（CAT） and concentrations of reactive oxygen species（ROS） and maldondialdehyde（MDA） were measured by different *** results showed that CdCl2 and nano-TiO2 at a low concen-tration of 0.75 total toxic unit（TU） exerted an additive effects on HO-1 gene expression,CAT activities and MDA *** the total TU was increased to 1 or 1.25 TU,the interaction was ***,the mixture with high proportion of CdCl2 produced an additive effect on the OGG1 gene expression,and the interaction was changed to be synergetic when the concentration of CdCl2 was lower than or equal to that of *** effects of CdCl2 and nano-TiO2 on cellular oxida-tive damage of HEK293T cells were found as indicated by the changes in the SOD activities and ROS *** was concluded that CdCl2 and nano-TiO2 exerts synergistic effects on the cellular oxidative damage of HEK293T cells,and the sensitivity of these indicators of oxidative damage varies with the proportion of CdCl2 and nano-TiO2 in the mixture.
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