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曲美布汀与复方消化酶治疗老年PDS型功能性消化不良对cgrp、NPSR1、IL-6的影响

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医学理论与实践 2024年第5期37卷 764-767页

作者：詹冰龙岩人民医院消化内科福建省龙岩市364000

目的:探讨曲美布汀与复方消化酶治疗老年餐后不适综合征(PDS)型功能性消化不良(FD)对血清指标、胃窦黏膜情况、胃动力学指标的影响及安全性。方法:纳入我院2021年1月—2022年12月老年PDS型FD患者98例,以抽签法将其等分为对照组(n=49)与... 详细信息

目的:探讨曲美布汀与复方消化酶治疗老年餐后不适综合征(PDS)型功能性消化不良(FD)对血清指标、胃窦黏膜情况、胃动力学指标的影响及安全性。方法:纳入我院2021年1月—2022年12月老年PDS型FD患者98例,以抽签法将其等分为对照组(n=49)与观察组(n=49)。对照组给予曲美布汀治疗,观察组给予曲美布汀联合复方消化酶治疗。对比两组治疗效果、血清指标(cgrp、NPSR1、IL-6)、胃窦黏膜情况[胃泌素(GAS)、生长抑素(SS)、胃动素(MTL)]、胃动力学指标(胃窦收缩频率、胃窦收缩幅度、胃窦面积收缩幅度、胃半排空时间)及不良反应发生率。结果:观察组治疗总有效率高于对照组(Pcgrp、IL-6水平低于对照组,NPSR1水平高于对照组(P0.05)。结论:曲美布汀与复方消化酶联合治疗老年PDS型FD效果更好,可改善血清cgrp、NPSR1、IL-6水平及胃窦黏膜情况、胃动力学情况,且不会明显增加药物不良反应。

关键词：曲美布汀复方消化酶餐后不适综合征功能性消化不良 cgrp NPSR1 胃动力学

壮医药线点灸治疗偏头痛急性期的疗效及对血清cgrp、IL-6、TNF-α的影响

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广西中医药 2024年第4期47卷 25-29页

作者：李陈玲尚昱志邢沙沙方刚广西国际壮医医院明秀分院广西南宁530001 广西中医药大学附属瑞康医院广西南宁530011

目的:观察壮医药线点灸治疗偏头痛急性期的临床疗效及对血清降钙素基因相关肽(cgrp)、白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)的影响。方法:选择70例偏头痛急性期患者为研究对象,按就诊先后顺序单盲随机分为试验组和对照组,每组35... 详细信息

目的:观察壮医药线点灸治疗偏头痛急性期的临床疗效及对血清降钙素基因相关肽(cgrp)、白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)的影响。方法:选择70例偏头痛急性期患者为研究对象,按就诊先后顺序单盲随机分为试验组和对照组,每组35例,试验组予壮医药线点灸治疗,对照组予口服西药盐酸氟桂利嗪胶囊治疗,两组均治疗4周。结果:试验组总有效率为94.29%,显著高于对照组的77.14%,差异有统计学意义(Pcgrp、IL-6、TNF-α水平及头痛强度积分、头痛发作次数积分、头痛持续时间积分、头痛症状总积分均较治疗前明显降低(Pcgrp释放、降低炎症因子(IL-6、TNF-α)水平有关。

关键词：壮医药线点灸偏头痛 cgrp IL-6 TNF-α 临床研究

Effects of TRPA1 activation and inhibition on TRPA1 and cgrp expression in dorsal root ganglion neurons

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Neural Regeneration Research 2019年第1期14卷 140-148页

作者： Xiao-Lei Wang Li-Wei Cui Zhen Liu Yue-Ming Gao Sheng Wang Hao Li Hu-Xiang Liu Ling-Jia Yu Department of Rheumatology Qilu Hospital of Shandong University Department of Respiratory Medicine Qilu Hospital of Shandong University Department of Orthopedics Qilu Hospital of Shandong University

Transient receptor potential ankyrin 1 （TRPA1） is a key player in pain and neurogenic inflammation, and is localized in nociceptive primary sensory dorsal root ganglion （DRG） neurons. TRPA1 plays a major role in the transmission of nociceptive sensory signals. The generation of neurogenic inflammation appears to involve TRPAl-evoked release of calcitonin gene-related peptide （cgrp）. However, it remains unknown whether TRPA1 or cgrp expression is affected by TRPA 1 activation. Thus, in this study, we examined TRPA 1 and cgrp expression in DRG neurons in vitro after treatment with the TRPA1 activator tbrmaldehyde or the TRPA1 blocker menthol. In addition, we examined the role of extracellular signal-regulated protein kinase 1/2 （ERK1/2） in this process. DRG neurons in culture were exposed to formaldehyde, menthol, the ERK1/2 inhibitor PD98059 ＋ formaldehyde, or PD98059 ＋ menthol. After treatment, real-time polymerase chain reaction, western blot assay and double immunofluorescence labeling were performed to evaluate TRPA1 and cgrp expression in DRG neurons. Formaldehyde elevated mRNA and protein levels of TRPA 1 and cgrp, as well as the proportion of TRPA1- and cgrp-positive neurons. In contrast, menthol reduced TRPA1 and cgrp expression. Furthermore, the effects of lbrmaldehyde, but not menthol, on cgrp expression were blocked by pretreatment with PD98059. PD98059 pretreatment did not affect TRPA1 expression in the presence of formaldehyde or menthol.

关键词： nerve regeneration TRPA1 TRPV1 formaldehyde menthol cgrp dorsal root ganglion neuron neurogenic inflammation nociceptive signal ERK1/2 neural regeneration

The neuropeptide cgrp enters the macrophage cytosol to suppress the NLRP3 inflammasome during pulmonary infection

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Cellular & Molecular Immunology 2023年第3期20卷 264-276页

作者： Fangrui Zhu Dou Yu Xiwen Qin Yan Qian Juan Ma Weitao Li Qiannv Liu Chunlei Wang Yan Zhang Yi Li Dong Jiang Shuo Wang Pengyan Xia Department of Immunology School of Basic Medical SciencesPeking University100191 BeijingChina NHC Key Laboratory of Medical Immunology Peking University100191 BejingChina Key Laboratory of Molecular Immunology Chinese Academy of Medical Sciences100191 BejingChina CAS Key Laboratory of Pathogen Microbiology and Immunology Institute of MicrobiologyChinese Academy of Sciences100101 BejingChina Center for Biosafety Mega-Science Chinese Academy of SciencesWuhanHubei 430071China Department of Anesthesiology Peking University Third Hospital100191 BeijingChina Department of Sports Medicine Peking University Third Hospital100191 BejingChina Beijing Key Laboratory of Sports Injuries Institute of Sports Medicine of Peking University100191 BejingChina

The NLRP3 inflammasome plays an essential role in resistance to bacterial infection. The nervous system secretes multiple neuropeptides affecting the nervous system as well as immune cells. The precise impact of the neuropeptide cgrp on NLRP3 inflammasome activation is still unclear. Here, we show that cgrp negatively regulates the antibacterial process of host cells. cgrp prevents NLRP3 inflammasome activation and reduces mature IL-1β secretion. Following NLRP3 inflammasome stimulation that triggers endosome leakage, cgrp internalized to endosomal compartments is released into the cell cytosol. Cytosolic cgrp binds directly to NLRP3 and dismantles the NLRP3-NEK7 complex, which is crucial for NLRP3 inflammasome activation. cgrp administration exacerbates bacterial infection, while the treatment with a cgrp antagonist has the opposite effect. Our study uncovers a unique role of cgrp in inhibiting inflammasome activation during infections, which might shed new light on antibacterial therapies in the future.

关键词： Neuropeptide cgrp NLRP3 Inflammasome Suppressor

Role of Receptor Component Protein （RCP） in cgrp and Angiotensin II on Vascular Peroxidase -1 Expression in Vascular Smooth Muscle Cell

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中国动脉硬化杂志 2013年第9期21卷 I0080-I0081页

作者： Yan-Mei Liu Hai-Yan Quan Gao-Feng Jiang Feng Guo Xu-Ping Qin (Institute of Pharmacy and Pharmacology University of South China Hengyang Hunan 421001 China)

Aim To determine the role of receptor component protein (RCP) in calcitonin gene-related peptide (cgrp) and angiotensin 11 sig- nal transduction in action of vascular peroxidase-1 ( VPO1 ) in VSMC. Methods Mouse vascular smooth muscle cell line A10 (A10VSMC) was employed in the study. Western blot was used to detect the expression of RCP and VPO1 protein. RT-PCR was used to detect the expression of RCP and VPO1mRNA. RCP-specific small interference RNA (RCP-siRNA) was used to silence oli- gonucleotide sequence, co-immunoprecipitation was used to determine interaction of RCP and G~xs/G[3/G~/. Results Both cgrp and angiotensin ]1 up-regulated the expression of RCP while cgrp down- regulated the expression of RCP induced by angioten- sinⅡin VSMC ; AngiotensinⅡup-regulated the expression of VPOI in a concentration and time-dependent manner in VSMC ; cgrp inhibited the expression of VPO1 induced by angiotensinⅡand the effect of cgrp was enhanced by Catalase, H:O:scavenger.

关键词：降钙素基因相关肽血管平滑肌细胞血管紧张素Ⅱ 过氧化物酶蛋白成分受体 RT-PCR法 cgrp

PKC and PKA mediate LPS-induced release of cgrp in DRG neurons of neonatal rats

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Acta Pharmacologica Sinica 1999年第5期 87-88页

作者： HOU Ling-Fei, WANG Xian(Institute of Vascular Medicine, Third Hospital, Beijing MedicalUniversity, Beijing 100083 , China)

Calcitonin gene-related peptide (cgrp), a 37-amino acid neuropeptide, is produced in dorsal root ganglia (DRG) neurons, which extend nerves peripherally to different organs and centrally to the spinal *** have previously reported that plasma levels of cgrp are significantly elevated in rats after injection of lipopolysaccharide (LPS) in *** triggers cgrp release from capsaicin-sensitive sensory nerves in the isolated mesenteric arteial bed of the *** this work, we used the cultured DRG neurons of neonatal mrs to detemine whether or not LPS could trigger cgrp release and if it did so, what cellular signaling pathway was involved in the cgrp *** were disassociated from the DRG of 5- 7-d neonatal rat using

关键词： cgrp DRG PKC and PKA mediate LPS-induced release of cgrp in DRG neurons of neonatal rats PKA

Modulation of cytokine and chemokine secretion from marine mono/macrophages by sensory neuropeptides cgrp and SP

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Acta Pharmacologica Sinica 1999年第5期 78-79页

作者： D REMICK Ann Arbor Department of Pathology MI 48109 USA University of Michigan Medical School

A lot of data have been published to prove that neuro-endocrine polypeptide hormones and neuropeptides can regulate theimmune response in which all the major immune cell types appear to be *** present review focuses on the immunomodulatory properties of calcitonin gene-related peptide (cgrp) and substance P (SP) which co-exist in primary afferent *** sensory nerve endings are in close contact with

关键词： cgrp Modulation of cytokine and chemokine secretion from marine mono/macrophages by sensory neuropeptides cgrp and SP

电针频率对大鼠脊髓灌流液中SOM和cgrp含量的影响

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生理学报 1998年第1期50卷 101-105页

作者：田津斌沈上韩济生北京医科大学神经科学研究中心北京100083

本研究采用放射免疫测定法，分析不同频率电针刺激下大鼠脊髓灌流液中生长抑素（SOM）和降钙素基因相关肽（cgrp）放免活性（ir）的变化。电针频率选择2，15和100H7；分别收集电针前、中、后各30min脊髓灌流液进行测定，实验结果如下：... 详细信息

本研究采用放射免疫测定法，分析不同频率电针刺激下大鼠脊髓灌流液中生长抑素（SOM）和降钙素基因相关肽（cgrp）放免活性（ir）的变化。电针频率选择2，15和100H7；分别收集电针前、中、后各30min脊髓灌流液进行测定，实验结果如下：（1）低频（2Hz）电计使脊髓灌流液中SOM－ir水平升高39％（P＜0.05），cgrp－ir降低47％（P＜0.05）；（2）中频电针（15Hz）则相反，使SOM-ri水平下降37％（P＜00.5），cgrp－ir水平增高92％（P＜0．.5）;（3）高频电针（100Hz）使SOM－ir水平减少30％（P＜0．01），但不影响cgrp－ir的水平。上述结果提示：特定频率的外周刺激可选择性地引起脊髓中SOM和cgrp释放的增强或抑制。这一发现可能有一定的临床意义。

关键词：生长抑素放免活性 cgrp 电针脊髓

降钙素基因相关肽(cgrp)mRNA在大鼠淋巴细胞中的表达(英文)

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生理学报 1998年第4期50卷 423-430页

作者：邢立愚邢宇彤唐跃明郭静萱王宪北京医科大学第三医院血管医学研究所北京100083

最近，我们研究发现大鼠胸腺和淋巴结淋巴细胞中存在降钙素基因相关肽（cgrp）样免疫反应活性。应用人工合成的可特异扩增降钙素／降钙素基因相关肽基因部分片断的寡核苷酸引物，通过逆转录-多聚酶链反应（RT－PCR），检测在大鼠脊髓背... 详细信息

最近，我们研究发现大鼠胸腺和淋巴结淋巴细胞中存在降钙素基因相关肽（cgrp）样免疫反应活性。应用人工合成的可特异扩增降钙素／降钙素基因相关肽基因部分片断的寡核苷酸引物，通过逆转录-多聚酶链反应（RT－PCR），检测在大鼠脊髓背根神经节、胸腺细胞及肠系膜淋巴结淋巴细胞中是否存在cgrpmRNA，进一步研究大鼠淋巴细胞能否合成cgrp。结果显示，通过RT－PCR从脊髓背根神经节（阳性对照）、胸腺和淋巴结淋巴细胞样品中，都检测到预期的90个碱基对的cgrpCDNA片断。这提示，cgrp除了是一种重要的神经递质外，也可在胸腺细胞和淋巴结淋巴细胞合成。此种cgrp可能与感觉神经源的一样，是免疫和神经系统的共用配基之一。淋巴细胞源的cgrp可能起着免疫细胞调节因子的作用。

关键词： cgrp 信使RNA 逆转录多聚酶链反应