Effects of TRPA1 activation and inhibition on TRPA1 and CGRP expression in dorsal root ganglion neurons

作     者：Xiao-Lei Wang Li-Wei Cui Zhen Liu Yue-Ming Gao Sheng Wang Hao Li Hu-Xiang Liu Ling-Jia Yu 

作者机构：Department of Rheumatology Qilu Hospital of Shandong University Department of Respiratory Medicine Qilu Hospital of Shandong University Department of Orthopedics Qilu Hospital of Shandong University 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2019年第14卷第1期

页      面：140-148页

核心收录：

学科分类：1002[医学-临床医学] 100204[医学-神经病学] 10[医学] 

基　　金：supported by the National Natural Science Foundation of China,No.81501935(to HL) the Natural Science Foundation of Shandong Province of China,No.ZR2014HQ065(to HL) 

主　　题：nerve regeneration TRPA1 TRPV1 formaldehyde menthol CGRP dorsal root ganglion neuron neurogenic inflammation nociceptive signal ERK1/2 neural regeneration 

摘      要：Transient receptor potential ankyrin 1 （TRPA1） is a key player in pain and neurogenic inflammation, and is localized in nociceptive primary sensory dorsal root ganglion （DRG） neurons. TRPA1 plays a major role in the transmission of nociceptive sensory signals. The generation of neurogenic inflammation appears to involve TRPAl-evoked release of calcitonin gene-related peptide （CGRP）. However, it remains unknown whether TRPA1 or CGRP expression is affected by TRPA 1 activation. Thus, in this study, we examined TRPA 1 and CGRP expression in DRG neurons in vitro after treatment with the TRPA1 activator tbrmaldehyde or the TRPA1 blocker menthol. In addition, we examined the role of extracellular signal-regulated protein kinase 1/2 （ERK1/2） in this process. DRG neurons in culture were exposed to formaldehyde, menthol, the ERK1/2 inhibitor PD98059 ＋ formaldehyde, or PD98059 ＋ menthol. After treatment, real-time polymerase chain reaction, western blot assay and double immunofluorescence labeling were performed to evaluate TRPA1 and CGRP expression in DRG neurons. Formaldehyde elevated mRNA and protein levels of TRPA 1 and CGRP, as well as the proportion of TRPA1- and CGRP-positive neurons. In contrast, menthol reduced TRPA1 and CGRP expression. Furthermore, the effects of lbrmaldehyde, but not menthol, on CGRP expression were blocked by pretreatment with PD98059. PD98059 pretreatment did not affect TRPA1 expression in the presence of formaldehyde or menthol.