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EFFEcT OF AcTIVE cOMPOUNDS ISOLATED FROM PTERIS SEMIPINNATA L ON DNA TOPOISOMERASES AND TYROSINE PROTEIN KINASE AND EXPRESSION OF c-myc IN LUNG ADENOcARcINOMA cELLS

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chinese Journal of cancer Research 2001年第2期13卷 105-109页

作者：李金华梁念慈莫丽儿张晓何承伟 Institute of Biochemistry and Molecular Biology Department of chemistry Guangdong Medical College

Objective: To study the effect of active compound 6F and A from Pteris semipinnata L.(PsL) on the activities of DNA topoisomerase (TOPO) I and II, activities of cytosolic and membrane TPK, and expression of oncogene c-myc in lung adenocarcinoma cells. Methods: The effect of compound 6F and A on activities of cytosolic and membrane TPK was measured by scintillation counting; the effect of compound A on expression of oncogene c-myc was determined by flow cytometry indirect fluorimetry. Results: compound 6F and A could inhibit the activities of TOPO I, and they strongly inhibited the TOPO II in 0.01 mg/L and 10.0 mg/L respectively. compound A slightly inhibited the activities of membrane TPK, but not the cytosolic one. compound A could inhibit the expression of oncogene c-myc. conclusion: Topoisomerases are target of compound 6F and A. compound A could slightly inhibit the activities of TPK, and showed an inhibitory effect on the expression of oncogene c-myc.

关键词： Pteris semipinnata L. DNA topoisomerase Tyrosine protein kinase c-myc

Roles of flanking sequences in the binding between unimolecular parallel-stranded G-quadruplexes and ligands

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Science Bulletin 2013年第7期58卷 731-744页

作者： GAI Wei YANG QianFan XIANG JunFeng SUN HongXia SHANG Qian LI Qian JIANG Wei GUAN AiJiao ZHANG Hong TANG YaLin XU GuangZhi Beijing National Laboratory for Molecular Sciences Center for Molecular SciencesState Key Laboratory for Structural Chemistry for Unstable and Stable SpeciesInstitute of ChemistryChinese Academy of Sciences Graduate University of chinese Academy of Sciences

G-quadruplexes attract more and more attention in recent years.Numerous small molecules which can induce or stabilize the formation of G-quadruplexes have been investigated on the purpose of anticancer drug development.As a motif existed in physiological condition,flanking sequences are an important part of G-quadruplexes but the study on the impact of flanking sequences on (G-quadruplex)-ligand binding is rarely reported.In this paper,the effects of flanking sequences on binding affinity between a series of unimolecular parallel-stranded G-quadruplex sequences derived from c-myc oncogene promoter (termed as c-myc G-quadruplexes) and their ligands are discussed in detail.The results showed that the flanking sequences on c-myc G-quadruplexes play key roles in (G-quadruplex)-ligand interaction.When a c-myc G-quadruplex is bound to its ligands,the flanking sequences might form a binding cavity above the terminal G-quartet,which could provide a suitable site for ligands to dock in.Moreover,the bases on flanking sequences could interact with ligand through π-π stacking,and finally form a sandwich-stacking mode (terminal G-quartet,ligand and bases on the flanking sequence).This mode could stabilize the (G-quadruplex)-ligand complex effectively and enhance the binding affinity dramatically.However,flanking sequences are also found to exhibit steric hindrance effect which could impede the (G-quadruplex)-ligand binding.

关键词： G-quadruplex flanking sequences interaction mechanism c-myc cyanine dye

THE RELATIONSHIP BETWEEN EXPRESSIONS OF N-myc AND c-myc ONcOGENES IN NEUROBLASTOMA:AN IN SITU HYBRIDIZATION AND IMMUNOcYTOcHEMIcAL STUDY

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chinese Medical Sciences Journal 1999年第2期14卷 102-106页

作者：折晓宁陈杰刘彤华张雷李佩娟王德田 DepartmentofPathology PekingUnionMedicalCollegeHospitalNationalLaboratoryofMedicalMolecularBiologyCAMS＆PUMCBeijing100730 DepartmentofPathology BeijingChildren＇sHospitalBeijing100045

ＩＮＴＲＯＤＵＣＴＩＯＮＮｅｕｒｏｂｌａｓｔｏｍａｏｒｉｇｉｎａｔｅｓｆｒｏｍｔｈｅｓｙｍｐａｔｈｏａｄ－ｒｅｎａｌｌｉｎｅａｇｅｏｆｔｈｅｎｅｕｒａｌｃｒｅｓｔａｎｄｉｔｉｓｔｈｅｍｏｓｔｃｏｍｍｏｎｅｘｔｒａ－ｃ... 详细信息

ＩＮＴＲＯＤＵＣＴＩＯＮＮｅｕｒｏｂｌａｓｔｏｍａｏｒｉｇｉｎａｔｅｓｆｒｏｍｔｈｅｓｙｍｐａｔｈｏａｄ－ｒｅｎａｌｌｉｎｅａｇｅｏｆｔｈｅｎｅｕｒａｌｃｒｅｓｔａｎｄｉｔｉｓｔｈｅｍｏｓｔｃｏｍｍｏｎｅｘｔｒａ－ｃｒａｎｉａｌｔｕｍｏｒｏｆｃｈｉｌ...

关键词： N-myc c-myc 相关性致癌基因神经细胞瘤免疫细胞化学

Anti-proliferative effects of a small molecule inhibitor of cDKAT7519 on chronic myeloid leukemia (cML) cells through haltingthe transition of cells from G2/M phase of the cell cycle

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BIOcELL 2020年第2期44卷 183-192页

作者： MASOUMEH OGHABI AVA SAFAROGHLI-AZAR ATIEH POURBAGHERI-SIGAROODI MOHAMMAD SAYYADI MOHSEN HAMIDPOUR MOHAMMAD HOSSEIN MOHAMMADI DAVOOD BASHASH Department of Hematology and Blood Banking School of Allied Medical SciencesShahid Beheshti University of Medical SciencesTehranIran Student Research committee Department of Hematology and Blood BankingSchool of Allied Medical SciencesShahid Beheshti University of Medical SciencesTehranIran School of Allied Medical Sciences Arak University of Medical SciencesArakIran

Pathogenesis of chronic myeloid leukemia(cML)has mostly been studied with regard to the oncogenic role of BcR/ABL fusion,however,recent disclosures have declared that the challenges with the treatment of cML patients would not be resolved until the role of other aberrancies is ignored.Given the involvement of cyclin-dependent kinases(cDKs)in the pathogenesis of cML,the present study aimed to investigate the effects of a multi-cDK inhibitor AT7519 on BcR/ABL-harboring cML-derived K562 cells.Our results showed that AT7519 effectively reduced the survival of K562 and induced its anti-proliferative effect through the induction of G2/M arrest due to elevated p21 and p27.The resulting data also revealed that either direct or indirect suppression of c-myc using specific c-myc inhibitor 10058-F4 and selective PI3K inhibitor cAL-101 resulted in a superior cytotoxicity,suggesting that the activation of PI3K pathway could attenuate antileukemic effects of the inhibitor,at least partly,through a c-mycdependent mechanism.To the best of our knowledge,to date,no study has addressed the effect of autophagy on cML cell response to AT7519,and,herein,we proposed for the first time that the suppression of autophagy boosted AT7519 cytotoxicity against K562.Overall,we suggested that selective cDK inhibitor AT7519 exerted antileukemic effect against cML cells and propose a novel therapeutic application for the inhibitor either as a single agent or in combination with c-myc and/or PI3K inhibitors.

关键词： chronic myeloid leukemia (cML) cyclin-dependent kinase (cDK) AT7519 Autophagy PI3K c-myc

SOX2、c-myc在晚期食管鳞状细胞癌组织中的表达及其临床意义

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现代肿瘤医学 2023年第6期31卷 1051-1055页

作者：王慧刘文颖新疆医科大学第一附属医院病理科新疆乌鲁木齐830054

目的:探讨SOX2、c-myc在晚期食管鳞状细胞癌组织中的表达情况及其与临床病理参数的关系。方法:收集我院病理科2008-2014年间64例晚期食管鳞状细胞癌患者资料,采用免疫组织化学EnVision法检测SOX2、c-myc的表达情况。结果:64例晚期食管... 详细信息

目的:探讨SOX2、c-myc在晚期食管鳞状细胞癌组织中的表达情况及其与临床病理参数的关系。方法:收集我院病理科2008-2014年间64例晚期食管鳞状细胞癌患者资料,采用免疫组织化学EnVision法检测SOX2、c-myc的表达情况。结果:64例晚期食管鳞癌患者中:男性51例(79.7%),女性13例(20.3%),男性患者远多于女性患者。发生了淋巴结转移的患者有44例,转移率为68.8%。患者远处转移率为51.6%(33/64),常见的转移部位有肺、肝、骨、胃、支气管等。免疫组化显示SOX2、c-myc均定位于细胞核,二者在晚期食管鳞癌组织中的阳性率分别为51.56%、25%。高表达的SOX2与远处转移密切相关(P=0.046),而与其他病理参数无关;c-myc表达升高与TNM分期密切相关(P=0.018),而与其他病理参数无关。SOX2、c-myc蛋白共表达与晚期食管鳞癌的TNM分期(P=0.020)、N分期(P=0.027)密切相关。结论:在晚期食管鳞状细胞癌中,高表达的SOX2与远处转移密切相关,希望能成为新的治疗靶点。

关键词：食管鳞状细胞癌晚期 SOX2 c-myc

DAB2IP通过调节ERα和β-catenin的相互作用促进前列腺癌进展的研究

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南开大学学报（自然科学版） 2022年第5期55卷 84-88页

作者：申永梅曹家松张琚石建党南开大学生命科学学院生物活性材料国家重点实验室天津300071

前列腺癌是男性常见的肿瘤疾病,雌激素受体α(estrogen receptorα,ERα)的表达在前列腺癌和激素抵抗性前列腺癌中升高,相反DAB2IP在癌组织中的表达降低,但是DAB2IP和ERα之间的调节关系并不清楚.RTPcR和Western blot方法验证在前列腺... 详细信息

前列腺癌是男性常见的肿瘤疾病,雌激素受体α(estrogen receptorα,ERα)的表达在前列腺癌和激素抵抗性前列腺癌中升高,相反DAB2IP在癌组织中的表达降低,但是DAB2IP和ERα之间的调节关系并不清楚.RTPcR和Western blot方法验证在前列腺癌细胞系Pc3中敲低DAB2IP可以使ERα的表达升高;免疫双荧光和co-IP方法验证ERα和β-catenin存在共表达并相互作用;RT-PcR和Western blot验证ERα可以调节和β-catenin下游基因c-myc的表达,并与c-myc启动子结合;此外,敲低DAB2IP可以使β-catenin的表达升高.推测在前列腺癌中,低表达的DAB2IP可能通过促进ERα和β-catenin的蛋白相互作用并结合c-myc启动子促进前列腺癌的进展.

关键词： DAB2IP ERα β-catenin c-myc

c-myc、PD-L1、cAIX在肺腺癌中表达及其临床意义

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中国老年学杂志 2023年第21期43卷 5175-5178页

作者：李子腾袁江李鹏冯凯滨州医学院第一临床医学院山东烟台264003 滨州市人民医院胸外科

目的研究原癌基因c-myc及其下游因子程序性死亡配体(PD-L)1、碳酸酐酶(cA)Ⅸ三者在肺腺癌和良性肺组织中的表达及其临床意义。方法通过免疫组织化学染色法检测52例肺腺癌病理标本及20例良性肺组织中c-myc、PD-L1、cAⅨ的表达,并分析其... 详细信息

目的研究原癌基因c-myc及其下游因子程序性死亡配体(PD-L)1、碳酸酐酶(cA)Ⅸ三者在肺腺癌和良性肺组织中的表达及其临床意义。方法通过免疫组织化学染色法检测52例肺腺癌病理标本及20例良性肺组织中c-myc、PD-L1、cAⅨ的表达,并分析其与肺腺癌临床病理特征的关系。结果c-myc、PD-L1、cAⅨ在肺腺癌和良性组织的阳性表达率分别为44.23%(23/52)、36.54%(19/52)、26.92%(14/52);15.00%(3/20)、0.00%(0/20)、0.00%(0/20),差异有统计学意义(Pc-myc、PD-L1、cAⅨ在肺腺癌组织中表达水平增高,三者的异常表达与肺腺癌的不良进展关系密切,c-myc可能通过调控PD-L1及cAⅨ蛋白的表达,促进肿瘤细胞免疫逃逸、营造细胞微环境酸中毒而促进肿瘤发展。

关键词：肺腺癌 c-myc 程序性死亡配体1 碳酸酐酶Ⅸ

乳腺癌中ING4与c-myc的表达及其临床意义

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临床与实验病理学杂志 2023年第5期39卷 601-604页

作者：周永清王先锋杜文杰庞景刘明玉俞群海南省三亚市妇幼保健院(三亚市妇女儿童医院)/上海儿童医学中心海南医院病理科三亚572000 安徽省铜陵市妇幼保健院病理科铜陵244000

目的探讨乳腺癌组织中ING4与c-myc的表达、两者的相关性、诊断及鉴别诊断。方法收集73例乳腺非特殊型浸润性癌临床资料,采用免疫组化EnVision两步法检测ING4与c-myc蛋白的表达,应用Spearman相关性分析两者与临床病理特征的关系及相关性... 详细信息

目的探讨乳腺癌组织中ING4与c-myc的表达、两者的相关性、诊断及鉴别诊断。方法收集73例乳腺非特殊型浸润性癌临床资料,采用免疫组化EnVision两步法检测ING4与c-myc蛋白的表达,应用Spearman相关性分析两者与临床病理特征的关系及相关性。结果73例乳腺非特殊型浸润性癌患者中,ING4蛋白阳性率为42.5%(31/73)明显低于癌旁正常组织(78.6%,P0.05)。乳腺非特殊型浸润性癌中c-myc蛋白阳性率为71.2%(52/73)明显高于癌旁正常组织(P0.05)。ING4蛋白与c-myc蛋白呈负相关(r=-0.372,Pc-myc阳性患者术后5年累积生存率低于c-myc阴性患者(Pc-myc与乳腺癌的发生、发展关系密切,两者表达呈负相关,可作为乳腺癌预后评估指标和潜在的生物学治疗靶点。

关键词：乳腺肿瘤 ING4 c-myc 临床病理特征

TELOMERASE AcTIVITY IN cOLOREcTAL cARcINOMA AND ITS cORRELATION WITH EXPRESSION OF c-myc

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chinese Journal of cancer Research 2005年第4期17卷 246-249页

作者：刘剑仑葛莲英张贵年 Department of Gastroenterological & Breast Surgery Department of Endoscope Affiliated Cancer Hospital of Guangxi Medical University Department of Gastrointestinal Surgery Guangxi Zhuang Autonomous Region Hospital

Objective： To study the role of telomerase activity and c-myc in pathogenesis and progression of colorectal carcinoma, and to investigate the possible regulatory mechanism of telomerase activation. Methods： A modified telomeric repeat amplification protocol （TRAP） and immunohistochemical staining was used to detect telomerase activity and the expression of c-myc in tissue samples from colorectal carcinoma, paracarcinomatousl tissues, normal mucosa, and adenomatoid polyp. Results： The positive rates of telomerase activity and c-myc expression were 83.33% and 80.00% in colorectal carcinoma, 13.33% and 23.33% in paracarcinomatousl tissues, 13.33% and 20.00% in normal mucosa, and 10.00% and 45.00% in adenomatoid polyp respectively, they were significantly higher in colorectal carcinoma than in paracarcinomatousl tissues, normal mucosa, and adenomatoid polyp （P〈0.05）. The rates of telomerase activity and c-myc expression were much higher in colorectal carcinoma with lymph nodes metastases than that without lymph nodes metastases. The expression of c-myc was found being significantly higher in the telomerase positive colorectal carcinoma than in the telomerase negative group （P〈0.05）. conclusion： The activation of telornerase and abnormal expression of c-myc might play an important role in the process of carcinogenesis and progression of colorectal carcinoma. The over-expression of c-myc may be related to telomerase activation and up-regulation in colorectal carcinoma.

关键词： colorectal carcinoma Telomerase activity c-myc