Roles of flanking sequences in the binding between unimolecular parallel-stranded G-quadruplexes and ligands
Roles of flanking sequences in the binding between unimolecular parallel-stranded G-quadruplexes and ligands作者机构：Beijing National Laboratory for Molecular SciencesCenter for Molecular SciencesState Key Laboratory for Structural Chemistry for Unstable and Stable SpeciesInstitute of ChemistryChinese Academy of Sciences Graduate University of Chinese Academy of Sciences
出 版 物：《Science Bulletin》 (科学通报(英文版))
年 卷 期：2013年第58卷第7期
页 面：731-744页
核心收录：
学科分类：0710[理学-生物学] 07[理学] 08[工学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种]
主 题：G-quadruplex flanking sequences interaction mechanism c-myc cyanine dye
摘 要：G-quadruplexes attract more and more attention in recent *** small molecules which can induce or stabilize the formation of G-quadruplexes have been investigated on the purpose of anticancer drug *** a motif existed in physiological condition,flanking sequences are an important part of G-quadruplexes but the study on the impact of flanking sequences on (G-quadruplex)-ligand binding is rarely *** this paper,the effects of flanking sequences on binding affinity between a series of unimolecular parallel-stranded G-quadruplex sequences derived from c-myc oncogene promoter (termed as c-myc G-quadruplexes) and their ligands are discussed in *** results showed that the flanking sequences on c-myc G-quadruplexes play key roles in (G-quadruplex)-ligand *** a c-myc G-quadruplex is bound to its ligands,the flanking sequences might form a binding cavity above the terminal G-quartet,which could provide a suitable site for ligands to dock ***,the bases on flanking sequences could interact with ligand through π-π stacking,and finally form a sandwich-stacking mode (terminal G-quartet,ligand and bases on the flanking sequence).This mode could stabilize the (G-quadruplex)-ligand complex effectively and enhance the binding affinity ***,flanking sequences are also found to exhibit steric hindrance effect which could impede the (G-quadruplex)-ligand binding.
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