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Effects of puerarin on burn injury pain mediated by P2X3 receptor

Effects of puerarin on burn injury pain mediated by P2X3 rec...

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中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： Chang-Shui XU,Jia-Ri LIN,Shuang-Mei LIU,Shang-Dong LIANG~* Department of Physiology,School of Basic Medical Sciences of Nanchang University,Nanchang 330006,China

Objective Peripheral administration of P2X agonists rapidly causes nocifensive behavior in experimental animals and pain sensation in *** of the nociceptive response to peripheral ATP is mediated by P2X *** shows that Puerarin may have anti-nociceptive response and the role of *** effects of Puerarin on rat burn injury pain mediated by P2X receptor was investigated at the present *** Superficial second degree foot/back burn injury models were ***-Dawley rat were randomly divided into sham paw burn group(SPB),puerarin-treated paw unburned control group(PPC),untreated superficial second degree paw burn group(UPB),puerarin-treated superficial second degree paw burn group(PPB),blank paw control group(BPC group),sham back burn group(SBB),puerarin-treated back unburned control group(PBC),untreated superficial second degree back burn group(UBB),puerarin-treated superficial second degree back burn group(PBB) and blank back control group(BBC).Mechanical withdrawal threshold(MWT)and thermal withdrawal latency (TWL)were measured and the expressions of P2X receptor in dorsal root ganglion from test rats were detected by immunohisto-chemistry,RT-PCR,Western blot and in situ *** MWT and TWL in group UPB and PPB were lower than those in group SPB,PPC and BPC(Pburn,and the MWT and TWL in group PPB were increased compared with those in group UPB after burn 48 h and 1 h respectively(P0.05),and there also was no difference in MWT and TWL between PPC and BPC(P>0.05).The MWT and TWL in group UPB were still decreased,there was difference between group UPB and group SPB(Pburn,the expressions of P2X protein and mRNA in dorsal root ganglion from burn injury model rat in group UPB were increased significantly compared with those in other gro

关键词： P2X3 receptor puerarin burn pain

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