Effects of puerarin on burn injury pain mediated by P2X3 receptor
会议名称：《中国神经科学学会第九届全国学术会议暨第五次会员代表大会》
会议日期：2011年
学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学]
基 金：supported by the grant(No30860086) from National Natural Science Foundation of China the grant (No20070403007) from Doctoral Fund of Ministry of Education of China the grant(No2008GZY0029) from Natural Science Foundation of Jiangxi Province the grant(NoGJJ11058) from the Educational Department of Jiangxi Province the grant(YBP08A01) from Jiangxi Province Excellent PhDStudents Foundation the grant(YC08B009) from the Innovation Foundation of Graduate School of Nanchang University the grant(2007A117) from the Health Department of Jiangxi Province
关 键 词：P2X3 receptor puerarin burn pain
摘 要：Objective Peripheral administration of P2X agonists rapidly causes nocifensive behavior in experimental animals and pain sensation in *** of the nociceptive response to peripheral ATP is mediated by P2X *** shows that Puerarin may have anti-nociceptive response and the role of *** effects of Puerarin on rat burn injury pain mediated by P2X receptor was investigated at the present *** Superficial second degree foot/back burn injury models were ***-Dawley rat were randomly divided into sham paw burn group(SPB),puerarin-treated paw unburned control group(PPC),untreated superficial second degree paw burn group(UPB),puerarin-treated superficial second degree paw burn group(PPB),blank paw control group(BPC group),sham back burn group(SBB),puerarin-treated back unburned control group(PBC),untreated superficial second degree back burn group(UBB),puerarin-treated superficial second degree back burn group(PBB) and blank back control group(BBC).Mechanical withdrawal threshold(MWT)and thermal withdrawal latency (TWL)were measured and the expressions of P2X receptor in dorsal root ganglion from test rats were detected by immunohisto-chemistry,RT-PCR,Western blot and in situ *** MWT and TWL in group UPB and PPB were lower than those in group SPB,PPC and BPC(P0.05),and there also was no difference in MWT and TWL between PPC and BPC(P0.05).The MWT and TWL in group UPB were still decreased,there was difference between group UPB and group SPB(P0.01),and there also was difference between PPC and BPC (P0.01).At day 3 post burn,the expressions of P2X protein and mRNA in dorsal root ganglion from burn injury model rat in group UPB were increased significantly compared with those in other gro