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broccoli sprout extract induces detoxification-related gene expression and attenuates acute liver injury

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World Journal of Gastroenterology 2015年第35期21卷 10091-10103页

作者： Kazutaka Yoshida Yusuke Ushida Tomoko Ishijima Hiroyuki Suganuma Takahiro Inakuma Nobuhiro Yajima Keiko Abe Yuji Nakai Research and Development Division Kagome Co.Ltd. Department of Applied Biological Chemistry Graduate School of Agricultural and Life ScienceThe University of Tokyo Faculty of Contemporary Human Life Science Department of Food and NutritionTezukayama University Institute for Food Science Hirosaki University

AIM: To investigate the effects of broccoli sprout extract(BSEx) on liver gene expression and acute liver injury in the ***: First, the effects of BSEx on liver gene expression were examined. Male rats were divided into two groups. The Control group was fed the AIN-76 diet, and the BSEx group was fed the AIN-76 diet containing BSEx. After a 10-d feeding period, rats were sacrificed and their livers were used for DNA microarray and realtime reverse transcription-polymerase chain reaction(RT-PCR) analyses. Next, the effects of BSEx on acute liver injury were examined. In experiments using acute liver injury models, 1000 mg/kg acetaminophen(APAP) or 350 mg/kg D-galactosamine(D-Gal N) was used to induce injury. These male rats were divided into four groups: Control, BSEx, Inducer(APAP or D-Gal N), and Inducer+BSEx. The feeding regimens were identical for the two analyses. Twenty-four hours following APAP administration via p.o. or D-Gal N administration via i.p., rats were sacrificed to determine serum aspartate transaminase(AST) and alanine transaminase(ALT) levels, hepatic glutathione(GSH) and thiobarbituric acid-reactive substances accumulation and glutathioneS-transferase(GST) activity. RESULTS: Microarray and real-time RT-PCR analyses revealed that BSEx upregulated the expression of genes related to detoxification and glutathione synthesis in normal rat liver. The levels of AST(70.91 ± 15.74 IU/m L vs 5614.41 ± 1997.83 IU/m L, P < 0.05) and ALT(11.78 ± 2.08 IU/m L vs 1297.71 ± 447.33 IU/m L, P < 0.05) were significantly suppressed in the APAP + BSEx group compared with the APAP group. The level of GSH(2.61 ± 0.75 nmol/g tissue vs 1.66 ± 0.59 nmol/g tissue, P < 0.05) and liver GST activity(93.19 ± 16.55 U/g tissue vs 51.90 ± 16.85 U/g tissue, P < 0.05) were significantly increased in the APAP + BSEx group compared with the APAP group. AST(4820.05 ± 3094.93 IU/m L vs 12465.63 ± 3223.97 IU/m L, P < 0.05) and ALT(1808.95 ± 1014.04 IU/m L vs 3936.46 ± 777.52 IU/

关键词： broccoli sprout Galactosamine Acute liver injury G

broccoli sprouts juice prevents lens protein aggregation in streptozotocin-induced diabetic rat

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International Journal of Ophthalmology(English edition) 2019年第9期12卷 1380-1385页

作者： Ayu Puspitasari Nina Handayani Department of Ophthalmology Faculty of Medicine Universitas Brawijaya Dr. Saiful Anwar General Hospital

AIM: To investigate the effect of broccoli sprouts juice in preventing lens protein aggregation in diabetic rat ***: Totally 25 male Wistar rats were divided into negative control group, diabetic group without juice treatment as positive control and diabetic group given broccoli sprouts juice 0.25, 0.5 and 1 g/d for 28 d. Diabetic rat model was obtained by administering a single dose streptozotocin 65 mg/kg intraperitoneal. At the end of the study, all rats were examined for fasting blood sugar level(FBS), visual cataract score using slit lamp biomicroscope and lens αB-crystallin expression using Western blot method. Statistical analysis was performed using one way ANOVA with post hoc test, correlation test, and simple linear ***: Positive control group had the highest cataract score and lens aggregated αB-crystallin expression. broccoli sprout juice dose of 1 g/d group had the mildest cataract score, as well as the expression of lens aggregated αB-crystallin compared to treatments groups 1 and 2, opposite to lens native αB-crystallin expression. The broccoli sprout juice groups gave a significant decrease in cataract score, and also in lens aggregated αB-crystallin expression in diabetic rat models(Pbroccoli sprout juice has a significant effect in preventing lens protein aggregation in diabetic rat model. The higher dose gives better visual cataract scores, lower lens aggregated αB-crystallin expression and higher lens native αB-crystallin expression.

关键词： diabetic cataract broccoli sprouts oxidative stress lens αB-crystallin rat

Effects of fresh-cut and storage on glucosinolates profile using broccoli as a case study

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Horticultural Plant Journal 2023年第2期9卷 285-292页

作者： Xiaoxin Huang Bing Cheng Yaqin Wang Guangmin Liu Liping Hu Xiaolu Yu Hongju He Institute of Agri-food Processing and Nutrition Beijing Academy of Agriculture and Forestry SciencesBeijing 100097China Beijing Vegetable Research Center Beijing Academy of Agriculture and Forestry SciencesBeijing 100097China School of Chinese Materia Medica Beijing University of Chinese MedicineBeijing 100029China

Glucosinolates(GLS) contribute to the unique flavour, nutrition, and plant defence of the Cruciferous vegetables. Understanding the GLS changes through postharvest processing is essential for defined preservation. In this study, four different fresh-cut types, whole flower(W),floret(F), quarterly cut floret(QF) and shredded floret(FS) of broccoli, were stored for 0, 1, 2 and 3 day(s) to explore GLS responses to postharvest treatments. As a result, seven GLS were identified, mainly including glucoraphanin(RAA), neoglucobrassicin(NEO), and glucobrassicin(GBC)and accounting for 52.69%, 20.12% and 14.99% of the total GLS(21.92 ± 0.48) μmol · g ^(-1 )DW, respectively. FS had the sharpest decrease in GLS after three days of storage(6.55 ± 0.37) μmol · g-1DW, while QF had the least(10.16 ± 0.33) μmol · g ^(-1 )DW. All GLS components decreased over storage, except for 4-methoxyglucobrassicin(4 ME) in FS and QF, suggesting its key role in serious wound defence. The results suggested certain postharvest approaches influenced the flavour and nutrition of broccoli.

关键词： Cruciferous vegetables broccoli Glucosinolate Wound stress Storage Fresh-cut

Enhanced resistance to Botrytis cinerea and Rhizoctonia solani in transgenic broccoli with a Trichoderma viride endochitinase gene

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Journal of Integrative Agriculture 2015年第3期14卷 430-437页

作者： YU Ya ZHANG Lei LIAN Wei-ran XU Feng-feng LI Shuang-tao XIANG Juan ZHANG Guo-zhen HU Zan-min ZHAO Bing REN Shu-xin GUO Yang-dong College of Agriculture & Biotechnology China Agricultural University Department of Biology Science & Technology Wuhan Institute of Bioengineering Institute of Genetics & Developmental Biology Chinese Academy of Sciences School of Agriculture Virginia State University

A endochitinase gene（Tch） from the fungus Trichoderma viride was introduced into broccoli（Brassica oleracea var. italica） by Agrobacterium-mediated transformation. Sixty-eight putative transformants were obtained and the presence of the Tch gene was confirmed by both PCR and Southern blot analysis. RT-PCR analysis showed an accumulation of the transcript encoding the endochitinase protein in the transgenic plants. Using real-time quantitative PCR, the expression profiling of endochitinase gene was analyzed. Primary transformants and selfed progeny were examined for expression of the endochitinase using a fluorometric assay and for their resistance to the pathogenic fungi Botrytis cinerea and Rhizoctonia solani. The endochitinase activities in T0 in vitro plants, T0 mature plants and T1 mature plants were correlated with leaf lesions, and the transgenic line T618 had high endochitinse activities of 102.68, 114.53 and 120.27 nmol L–1 MU min–1 mg–1 protein in the three kinds of plants, respectively. The endochitinase activity showed a positive correlation with the resistance to the pathogens. Most transgenic T0 broccoli had increased resistance to the pathogens of B. cinerea and R. solani in leaf assays and this resistance was confirmed to be inheritable. These findings suggested that expression of the Tch gene from T. viride could enhance resistance to pathogenic fungi in Brassica species.

关键词： Botrytis cinerea broccoli endochitinase gene genetic transformation Rhizoctonia solani

Genetic diversity and population structure analysis of 161 broccoli cultivars based on SNP markers

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Horticultural Plant Journal 2021年第5期7卷 423-433页

作者： Jingjing Huang Yumei Liu Fengqing Han Zhiyuan Fang Limei Yang Mu Zhuang Yangyong Zhang Honghao Lv Yong Wang Jialei Ji Zhansheng Li Institute of Vegetables and Flowers Chinese Academy of Agricultural SciencesKey Laboratory of Biology and Genetic Improvement of Horticultural Crops of the Ministry of AgricultureBeijing 100081China

To better understand the genetic diversity and population structure of broccoli cultivars planted in China,a total of 161 representative broccoli cultivars in the past 25 years were collected and analysed based on single nucleotide polymorphism(SNP)*** pairs of primers with good polymorphism and high resolution were screened from 315 pairs of SNP primers by 3 broccoli accessions(inbred lines)with different phenotypes and *** 10 pairs of SNP primers were selected,producing 78 *** diversity analysis indicated that the polymorphism information content(PIC)of SNP primer ranged from 0.64 to *** observed number of alleles(Na)was 2.00,the effective number of alleles(Ne)was 1.11–2.00,the Nei’s gene diversity(H)was 0.10–0.50,and Shannon information index(I)was 0.20–0.70 using PopGene32 *** clustering results showed that the 161 broccoli cultivars could be divided into 4 major subgroups(A,B,C and D),foreign cultivars were all assigned to subgroup A,and domestic cultivars were assigned to 3 subgroups of B,C,and *** study indicated that some domestic cultivars and foreign cultivars were similar in genetic background,but most domestic cultivars were still different from the Japanese *** K=2,the population structure result presented that 161 broccoli cultivars could be divided into 1 simple group(2 groups)and 1 mixed *** Q≥0.6,143(88.82%)broccoli cultivars belonged to the simple *** simple groups 68(42.24%)broccoli cultivars of group 1 were derived from Japan,the United States,Switzerland,the Netherlands,China-Taiwan,and China-Mainland;75(46.58%)broccoli cultivars belonged to group 2;when Qbroccoli cultivars belonged to the mixed *** study is helpful to understand the diversity and resolution of broccoli cultivars from worldwide,which is beneficial to plant breeding and materials *** meanwhile,this result is also used for construction of broccoli fingerprint serving for cultivar id

关键词： broccoli Genetic diversity Population structure analysis Fingerprint SNP

Analysis of anticancer compound,indole-3-carbinol,in broccoli using a new ultrasound-assisted dispersive-filter extraction method based on poly(deep eutectic solvent)-graphene oxide nanocomposite

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Journal of Pharmaceutical Analysis 2022年第2期12卷 301-307页

作者： Yanan Yuan Huanhuan Chen Yehong Han Fengxia Qiao Hongyuan Yan Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of Ministry of Education College of Pharmaceutical ScienceHebei UniversityBaodingHebei071002China Key Laboratory of Public Health Safety of Hebei Province Institute of Life Science and Green DevelopmentCollege of Public HealthHebei UniversityBaodingHebei071002China College of Biochemistry Baoding UniversityBaodingHebei071000China

Indole-3-carbinol(I3C),an important anticancer compound found in broccoli,has attracted considerable *** rapid extraction and accurate analysis of I3C in the pharmaceutical industry in broccoli is challenging as I3C is unstable at low pH and high *** this study,a rapid,accurate,and lowcost ultrasound-assisted dispersive-filter extraction(UADFE)technique based on poly(deep eutectic solvent)-graphene oxide(PDES-GO)adsorbent was developed for the isolation and analysis of I3C in broccoli for the first ***-GO with multiple adsorption interactions and a fast mass transfer rate was synthesized to accelerate adsorption and *** was developed by combining dispersive solid-phase extraction(DSPE)and filter solid-phase extraction(FSPE)to realize rapid extraction and *** on the above two strategies,the proposed PDES-GO-UADFE method coupled with high-performance liquid chromatography(HPLC)allowed the rapid(15-16 min),accurate(84.3%-96.4%),and low-cost(adsorbent:3.00 mg)analysis of I3C in broccoli and was superior to solid-phase extraction,DSPE,and FSPE *** proposed method showed remarkable linearity(r=0.9998;range:0.0840-48.0 mg/g),low limit of quantification(0.0840 mg/g),and high precision(relative standard deviation<5.6%).Therefore,the PDES-GO-UADFE-HPLC method shows significant potential in the field of pharmaceutical analysis for the separation and analysis of anti-cancer compounds in complex plant samples.

关键词： Indole-3-carbinol broccoli Poly(deep eutectic solvent)-graphene oxide Ultrasound-assisted dispersive-filter extraction Rapid analysis

Overexpression of a broccoli Defensin Gene BoDFN Enhances Downy Mildew Resistance

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Journal of Integrative Agriculture 2012年第7期11卷 1137-1144页

作者： JIANG Ming HE Cai-ming MIAO Li-xiang ZHANG Yu-chao College of Life Science Taizhou University Linhai 317000 P.R.China Institute of Horticulture Zhejiang Academy of Agricultural Science Hangzhou 310021 P.R. China

Plant defensins are small, basic cysteine-rich peptides that play important roles in disease resistance. A gene, designated BoDFN, was isolated from Brassica oleracea var. italica. Gene sequence has been submitted to NCBI with an accession no. of HQ436486. Complete coding sequence of BoDFN is 243 bp in length encoding 80 amino acids. Sequence comparison results showed that BoDFN shared high homology with those of crucifer plants and there were only few DNA base differences. RT-PCR results indicated an increase of gene expression in Hyaloperonospora parasitica infected leaves and revealed a significant increase at 24 and 36 h of treatment. A recombinant plasmid, named pBI121-BoDFN, was constructed and introduced into Agrobacterium tumefacien LBA4404. PCR screening for 65 regenerated plantlets, 17 positive plantlets were obtained, PCR screening results revealed that 17 out of 65 regenerated plantlets were positive. Disease resistant identification results indicated that all positive plants showed an increase in resistance to H. parasitica.

关键词： broccoli downy mildew defensin overexpression

Effects of different cooking methods on health-promoting compounds of broccoli

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2009年第8期10卷 580-588页

作者： Gao-feng YUAN Bo SUN Jing YUAN Qiao-mei WANG Department of Horticulture Zhejiang University Hangzhou 310029 China Key Laboratory of Horticultural Plant Growth Development and Quality Improvement Ministry of Agriculture Hangzhou 310029 China

The effects of five domestic cooking methods,including steaming,microwaving,boiling,stir-frying,and stir-frying followed by boiling(stir-frying/boiling),on the nutrients and health-promoting compounds of broccoli were *** results show that all cooking treatments,except steaming,caused significant losses of chlorophyll and vitamin C and significant decreases of total soluble proteins and soluble *** aliphatic and indole glucosinolates were significantly modified by all cooking treatments but not by *** general,the steaming led to the lowest loss of total glucosinolates,while stir-frying and stir-frying/boiling presented the highest ***-frying and stir-frying/boiling,the two most popular methods for most homemade dishes in China,cause great losses of chlorophyll,soluble protein,soluble sugar,vitamin C,and glucosinolates,but the steaming method appears the best in retention of the nutrients in cooking broccoli.

关键词： broccoli Cooking Glucosinolates Vitamin C Chlorophyll Soluble sugar

Simulation approach for optimal design of vacuum cooling on broccoli by simulated annealing technique

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International Journal of Agricultural and Biological Engineering 2014年第5期7卷 111-115页

作者： Ding Tian W.A.L.Alves S.A.Araujo J.C.C.Santana Ling Jiangang Chen Jianchu Liu Donghong Department of Food Science and Nutrition School of Biosystems Engineering and Food ScienceZhejiang UniversityHangzhou 310058China Industrial Engineering Post Graduation Program Nine July University05001-100São PauloBrazil Ningbo Academy of Agricultural Sciences Ningbo 315040P.R.China Fuli Institute of Food Science Zhejiang UniversityHangzhou 310058P.R.China

Water-spraying is regarded as an effective method to reduce the weight loss of product during vacuum cooling *** study aimed to investigate the effect of vacuum cooling factors on the weight loss of postharvest broccoli,and attempted to optimize the treatment conditions by simulated annealing(SA)*** algorithm based on simulated annealing meta-heuristic technique was developed to identify the optimum condition for vacuum cooling treatment of *** demonstrated that the SA algorithm could adjust well with the simulation of the broccoli cooling *** best condition was at 200 Pa of pressure,274 g broccoli,6% of water volume and 40 min processing *** this condition,a product with only 0.35% of weight loss and 1.48℃ final temperature was *** developed method may help to effectively control the weight loss during vacuum cooling process and reducing the economic loss.

关键词： broccoli vacuum cooling simulated annealing optimization