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A broad spectrum Identification Approach for Two Generations of epsps GM Soybeans

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Asian Agricultural Research 2017年第12期9卷 59-63页

作者： Qiuchi SONG Xiwei LIU Yiwei MOU Yuan JING Jun SONG Class 10 Grade 2018 Wenmiao School of Shishi High School Teaching and Research Group of Biology Wenmiao School of Shishi High School Quality and Safety Center of Agricultural Products of Ganzi Prefecture of Sichuan Province Analysis and Determination Center Sichuan Academy of Agricultural Sciences

In order to accurately identify the first and second generations of epsps genetically modified(GM) soybeans and related products,a broad spectrum identification approach was established using the real time Polymerase Chain Reaction(PCR) principle according to the homology of epsps genes of the first and second generations of GM soybeans.A pair of primer and probe was designed to simultaneously identify exogenous gene epsps of two generations of GM ***,evaluation was carried out on this approach from the accuracy,specificity,sensitivity and *** experimental results indicated that(ⅰ) although there is certain difference in epsps gene sequence between the first and second generations of epsps genetically modified(GM) soybeans,the established approach can simultaneously detect the epsps genes of the bean curd using two generations of soybean as raw materials;(ⅱ) in the accuracy and specificity experiment,only cp4-epsps genes of two generations of GM soybeans were detected,so this approach has high specificity and accuracy;(ⅲ) in the experiment of 5 copies of epsps genes of 40 repeated identification reaction systems,5 copies of epsps genes can be detected each time,therefore at 100% confidence level,this approach can identify 5 copies of epsps genes,showing that this approach has high sensitivity and reproducibility.

关键词： Genetically modified(GM) soybeans epsps gene broad spectrum Specificity Sensitivity

High efficient broad-spectrum Bradyrhizobium elkanii Y63-1

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Oil Crop Science 2023年第4期8卷 228-235页

作者： Piao Leng Fuxiao Jin Song Li Yi Huang Chanjuan Zhang Zhihui Shan Zhonglu Yang Limiao Chen Dong Cao Qingnan Hao Wei Guo Hongli Yang Shuilian Chen Xinan Zhou Songli Yuan Haifeng Chen Oil Crops Research Institute of Chinese Academy of Agricultural Sciences/Key Laboratory of Biology and Genetic Improvement of Oil Crops Ministry of Agriculture and Rural AffairsWuhan430062China

Soybean(Glycine max),the primary source of high-quality plant protein,plays a crucial role as a grain and oil crop in *** the full potential of symbiotic nitrogen fixation in soybean production holds immense significance for agriculture and ecology *** 63,a newly developed early-maturing summer soybean cultivar in 2021,exhibits remarkable traits such as high yield,superior quality,multi-resistance,and wide *** this study,eight distinct rhizobia strains from diverse regions were meticulously screened to identify highly effective strains specifically suited for Zhongdou *** aboveground biomass,plant height,chlorophyll content,root length,nodule number,and nodule dry weight of Zhongdou 63 were measured and the data were subjected to statistical *** results demonstrated that Y63-1 is a predominant strain of Zhongdou ***,we conducted further investigations on the broad-spectrum nodulation characteristics of *** representative soybean cultivars were individually inoculated with Y63-1 and subsequently analyzed for nodule numbers and nodule dry weight in their symbiotic systems with *** findings revealed that Y63-1 effectively formed nodules with all ten soybean varieties *** summary,our current study identified highly efficient broad-spectrum Bradyrhizobium elkanii strain Y63-1 as the predominant strain in Zhongdou 63 and provided a theoretical foundation for enhancing yield potential not only in Zhongdou 63 but also in other varieties through inoculation with highly efficient rhizobia in production.

关键词： Soybean Rhizobium Nodule symbiosis Nodulation broad spectrum

High harmonic generation in crystal SiO_(2) by sub-10-fs laser pulses

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Chinese Physics B 2023年第6期32卷 213-217页

作者：王帅郭嘉为贺新奎梁玥瑛谢百川钟诗阳滕浩魏志义 Beijing National Laboratory for Condensed Matter Physics Institute of PhysicsChinese Academy of SciencesBeijing 100190China University of Chinese Academy of Sciences Beijing 100490China Songshan Lake Materials Laboratory Dongguan 523808China School of Physics Nankai UniversityTianjin 300071China

The high harmonic generation(HHG)by few-cycle laser pulses is essential for research in strong-field solid-state *** comparison of high harmonic spectra of solids generated by laser pulses with varying durations,we discovered that lasers with good dispersion compensation are capable of producing a broad spectrum of high *** the pulse duration is further compressed,several interference peaks appear in the broad ***,we conducted simulations using the semiconductor Bloch equation,considering the effect of Berry curvature,to better understand this *** work provides a valuable approach for studying HHG by few-cycle laser pulses in solid materials,expanding the application of HHG in attosecond physics.

关键词： solid state hhg few-cycle laser pulses broad spectrum interference peak

Emerging drug design strategies in anti-influenza drug discovery

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Acta Pharmaceutica Sinica B 2023年第12期13卷 4715-4732页

作者： Chuanfeng Liu Lide Hu Guanyu Dong Ying Zhang Edeildo Ferreira da Silva-Jinior Xinyong Liu Luis Menendez-Arias Peng Zhan Department of Medicinal Chemistry Key Laboratory of Chemical Biology(Ministry of Education)School of Pharmaceutical SciencesShandong UniversityJinan 250012China Laboratory of Medicinal Chemistry Institute of Pharmaceutical SciencesFederal University of AlagoasMaceio 57072-970AlagoasBrazil Centro de Biologia Molecular“Severo Ochoa”(Consejo Superior de Investigaciones Cientificas&Universidad Autonoma de Madrid) Madrid 28049Spain

Influenza is an acute respiratory infection caused by influenza viruses(IFV),According to the World Health Organization(WHO),seasonal IFV epidemics result in approximately 3-5 million cases of severe illness,leading to about half a million deaths worldwide,along with severe economic losses and social ***,frequent mutations in IFV lead to a certain lag in vaccine development as well as resistance to existing antiviral ***,it is of great importance to develop anti-IFV drugs with high efficiency against wild-type and resistant strains,needed in the fight against current and future outbreaks caused by different IFV *** this review,we summarize general strategies used for the discovery and development of antiviral agents targeting multiple IFV strains(including those resistant to available drugs).Structure-based drug design,mechanism-based drug design,multivalent interaction-based drug design and drug repurposing are amongst the most relevant strategies that provide a framework for the development of antiviral drugs targeting IFV.

关键词： Influenza virus Drug design strategies Anti-drugresistance High efficiency broad spectrum

A low-dimension structure strategy for flexible photodetectors based on perovskite nanosheets/ZnO nanowires with broadband photoresponse

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Science China Materials 2020年第1期63卷 100-109页

作者： Shalong Wang Zhengfeng Zhu Yousheng Zou Yuhang Dong Shuting Liu Jie Xue Leimeng Xu Yuhui Dong Jizhong Song Key Laboratory of Advanced Display Materials and Devices Ministry of Industry and Information TechnologyCollege of Material Science and EngineeringNanjing University of Science and TechnologyNanjing 210094China

Flexible photodetectors(PDs) have huge potential for application in next-generation optoelectronic devices due to their lightweight design, portability, and excellent large area compatibility. The main challenge in the construction of flexible PDs is to maintain the optoelectronic performance during repetitive bending, folding and ***, flexible PDs based on ZnO nanowires(NWs) and CsPbBr3 nanosheets(NSs) were constructed by an integrated low-dimensional structure strategy. Benefiting from the flexibility of unique sheet and wire structures, the PDs were able to maintain excellent operational stability under various mechanical stresses. For example, the PDs exhibited no obvious changes in optoelectronic performance after bending for 1000 times. Additionally, the PDs exhibited an integrated broadband response ranging from ultraviolet to visible region due to the combination of the intrinsic light absorption capability of ZnO and CsPbBr3. The PDs demonstrated high responsivities of 3.10 and 0.97 A W^-1 and detectivities of 5.57×10^12 and1.71×10^12 Jones under ultraviolet and visible light irradiation,respectively. The proposed construction strategy for highly flexible and performance-integrated PDs shows great potential in future smart, wearable optoelectronic devices.

关键词： perovskite ZnO nanowires flexible photodetectors broad spectrum