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Study on PVL, blaoxa-23 and blaoxa-51 Genes in Drug Resistant Staphylococcus aureus Causing Surgical-Sites and Traumatic Wounds Infections, Sudan

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American Journal of Molecular Biology 2022年第2期12卷 30-42页

作者： Sanaa Mohammed Yousif Adam Dawoud Abakar Salaheldein Gumaa Elzaki Salma Omer Ibrahim Omer Abu Elhasan Mohamed Taj-Eldin Elhadi Abdalla Ahmed National Blood Bank Ministry of Health Khartoum Sudan Department of Medical Parasitology Faculty of Medical Laboratory Sciences University of Gezira Wad Medani Sudan Molecular Biology Laboratory Department of Epidemiology Tropical Medicine Research Institute Khartoum Sudan Department of Medical Microbiology Faculty of Medical Laboratory Sciences University of Gezira Wad Medani Sudan Wad Medani College of Medical Sciences and Technology Wad Medani Sudan

Background: The characteristics of Staphylococcus aureus that made it the most important cause of wound infections are environmental spread antimicrobials resistance and virulence. Absence of molecular detection of drug resistance and virulence factors in many developing countries limits the epidemiological information. This study conducted to identify PVL virulence gene, and blaoxa-23 and blaoxa-51 drug resistance genes of Staphylococcus aureus isolated from surgical-sites infections (SSIs) and traumatic wounds. Methods: A cross-sectional study was conducted from 2019 to 2021, in which 70 cefepime resistant Staphylococcus aureus were used, the strains were isolated from patients of SSIs and traumatic wounds admitted to the department of General Surgery in Wad Medani Teaching Hospital. Mannitol salt agar was used for primary culture followed by biochemical identification and Kirby Bauer susceptibility testing. Single and multiplex PCR protocols performed for bacterial confirmation and target genes detection. Results: Staphylococcus aureus strains from SSIs constituted 56% (39/70) from which 41% (16/39) possessed PVL gene while 42% (13/31) of wound infections strains were positive for PVL gene. Presence of PVL gene was significantly associated with resistance to meropenem (P. value 0.023) and ceftriaxone (P. value 0.037). blaoxa-23 was significantly detected with resistance to meropenem, augmentin and ceftriaxone. While blaoxa-51 was significantly identified among Staphylococcus aureus strains that showed resistance to meropenem and ciprofloxacin. Conclusion: This is the first study in Sudan that identified blaoxa-23 and blaoxa-51 in Staphylococcus aureus and correlated them to resistance to commonly used antimicrobials. Meropenem resistant Staphylococcus aureus were significantly positive for PVL, blaoxa-23 and baOXA-51 genes.

关键词： Staphylococcus aureus PVL blaoxa-23 blaoxa-51 Wounds Sudan

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Rapid and accurate detection of carbapenem-resistance gene by isothermal amplification in Acinetobacter baumannii

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Burns & Trauma 2020年第1期8卷 136-147页

作者： Shuang Liu Guangtao Huang Yali Gong Xiaojun Jin Yudan Meng Yizhi Peng Junning Zhao Xiaolu Li Qin Li Department of Plastic&Burns Surgery The Affiliated Hospital of Southwest Medical UniversityTai Ping StreetLuzhou646000China Department of Dermatology Dazhou Central HospitalNanyue Temple StreetDazhou635000China Institute of Burn Research Southwest HospitalThe Army Medical UniversityGao Tan Yan StreetChongqing400038China Department of Emergency The First Affiliated HospitalZhejiang University School of MedicineQingchun RoadHangzhou310003China School of Medicine Chengdu UniversityXindu AvenueChengdu 610106China Sichuan Translational Medicine Center of Chinese Medicine Sichuan Academy of Chinese Medical SciencesRen Min Nan Lu RoadChengdu610041China Department of Infectious Diseases The Affiliated Hospital of Southwest Medical UniversityTai Ping StreetLuzhou646000China

Background:Acinetobacter baumannii(***)is one of the pivotal pathogens responsible for nosocomial infections,especially in patients with low immune response,and infection with carbapenem-resistant *** has been increasing in recent *** and accurate detection of carbapenem-resistance genes in *** could be of immense help to clinical ***:In this study,a 15-μL reaction system for recombinase polymerase amplification(RPA)was developed and *** collected 30 clinical isolates of *** from the Burn Institute of Southwest Hospital of Third Military Medical University(Army Medical University)for 6 months and tested antibiotic susceptibility using the VITEK 2 *** was detected based on the blaoxa-51 gene by PCR,qPCR and 15μL-RPA,*** and specificity were *** addition,PCR and 15μL-RPA data for detecting the carbapenem-resistance gene blaoxa-23 were comparatively ***:The detection limit of the blaoxa-51 gene by 15μL RPA was 2.86 CFU/ml,with sensitivity comparable to PCR and *** positive amplification signals were detected in non-Acinetobacter isolates,indicating high ***,only 18 minutes were needed for the 15μL RPA ***,an antibiotic susceptibility test showed that up to 90%of *** strains were resistant to meropenem and imipenem;15μL RPA data for detecting blaoxa-23 showed that only 10%(n=3)of *** isolates did not show positive amplification signals,and the other 90%of(n=27)isolates were positive,corroborating PCR ***:We demonstrated that the new 15μL RPA assay for detecting blaoxa-23 in *** is faster and simpler than qPCR and *** is a promising alternative molecular diagnostic tool for rapid and effective detection of *** and drug-resistance genes in the field and point-ofcare testing.

关键词： Acinetobacter baumannii Recombinase polymerase amplification Rapid detection Carbapenem-resistance gene blaoxa-51 blaoxa-23

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