Rapid and accurate detection of carbapenem-resistance gene by isothermal amplification in Acinetobacter baumannii

作     者：Shuang Liu Guangtao Huang Yali Gong Xiaojun Jin Yudan Meng Yizhi Peng Junning Zhao Xiaolu Li Qin Li 

作者机构：Department of Plastic&Burns SurgeryThe Affiliated Hospital of Southwest Medical UniversityTai Ping StreetLuzhou646000China Department of DermatologyDazhou Central HospitalNanyue Temple StreetDazhou635000China Institute of Burn ResearchSouthwest HospitalThe Army Medical UniversityGao Tan Yan StreetChongqing400038China Department of EmergencyThe First Affiliated HospitalZhejiang University School of MedicineQingchun RoadHangzhou310003China School of MedicineChengdu UniversityXindu AvenueChengdu 610106China Sichuan Translational Medicine Center of Chinese MedicineSichuan Academy of Chinese Medical SciencesRen Min Nan Lu RoadChengdu610041China Department of Infectious DiseasesThe Affiliated Hospital of Southwest Medical UniversityTai Ping StreetLuzhou646000China 

出 版 物：《Burns & Trauma》 (烧伤与创伤（英文）)

年 卷 期：2020年第8卷第1期

页      面：136-147页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：Science and Technology Research of Chinese Medical Sciences of Sichuan Province Pharmaceutical Administration [2015Z006] Key Field Technology Innovation of Southwest Hospital [SWH2016ZDCX1001] National Natural Science Foundation of China [81772073, 81571896] 

主　　题：Acinetobacter baumannii Recombinase polymerase amplification Rapid detection Carbapenem-resistance gene blaOXA-51 blaOXA-23 

摘      要：Background:Acinetobacter baumannii(***)is one of the pivotal pathogens responsible for nosocomial infections,especially in patients with low immune response,and infection with carbapenem-resistant *** has been increasing in recent *** and accurate detection of carbapenem-resistance genes in *** could be of immense help to clinical ***:In this study,a 15-μL reaction system for recombinase polymerase amplification(RPA)was developed and *** collected 30 clinical isolates of *** from the Burn Institute of Southwest Hospital of Third Military Medical University(Army Medical University)for 6 months and tested antibiotic susceptibility using the VITEK 2 *** was detected based on the blaOXA-51 gene by PCR,qPCR and 15μL-RPA,*** and specificity were *** addition,PCR and 15μL-RPA data for detecting the carbapenem-resistance gene blaOXA-23 were comparatively ***:The detection limit of the blaOXA-51 gene by 15μL RPA was 2.86 CFU/ml,with sensitivity comparable to PCR and *** positive amplification signals were detected in non-Acinetobacter isolates,indicating high ***,only 18 minutes were needed for the 15μL RPA ***,an antibiotic susceptibility test showed that up to 90%of *** strains were resistant to meropenem and imipenem;15μL RPA data for detecting blaOXA-23 showed that only 10%(n=3)of *** isolates did not show positive amplification signals,and the other 90%of(n=27)isolates were positive,corroborating PCR ***:We demonstrated that the new 15μL RPA assay for detecting blaOXA-23 in *** is faster and simpler than qPCR and *** is a promising alternative molecular diagnostic tool for rapid and effective detection of *** and drug-resistance genes in the field and point-ofcare testing.