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Label-free Detection of biotin Using Nanoporous TiO2/DNA Thin-film Coated Wavelength Interrogated Surface Plasmon Resonance Sensors

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Chemical Research in Chinese Universities 2014年第1期30卷 157-162页

作者： LI Qiushun HE Hao WANG Junnan ZHENG Hui SHI Jianguo LI Mingyu DONG Wenfei QI Zhimei Key Laboratory of Biosensor of Shandong Province Biology Institute of Shandong Academy of Sciences Jinan 250014 P R. China Suzhou Institute of Biomedical Engineering and Technology Chinese Academy of Sciences Suzhou 215163 P. R. China The Second Hospital of Jilin University Changchun 130041 P. R. China State Key Laboratory of Modern Optical Instrumentation Zhejiang University Hangzhou 310027 P. R. China State Key Laboratory of Transducer Technology Institute of Electronics Chinese Academy of Sciences Beijing 100190 P. R. China

The sensing sensitivity of the wavelength interrogated surface plasmon resonance（WISPR） biosensor could be improved by self-assembly of nanoporous thin-film of TiO2 nanoparticles/DNA（TiO2/DNA）n（n is the number of bilayer） on wavelength interrogated surface plasmon resonance（WISPR） *** growth behavior and surface structure of the nanoporous thin-film were investigated by UV-Vis spectroscopy and scanning electron microscopy,*** home-made WISPR sensor with Krestchmann configuration consisted of a tungsten-halogen lamp as a photon source and a CCD camera as the *** the deposition of （DNA/TiO2）n thin film on WISPR chips,the resonance peak of the reflection spectra appeared in *** the increases of n,the resonance wavelength gradually red shifted,which is consistent with the simulated *** the optimization of the porous film,the WISPR biosensor was utilized to detect low-molecular-weight analytes,such as *** result demonstrates that the sensitivity of [poly（styrene sulfonate）/polyally lamine hydrochlorides]5（PSS/PAH）5 could be 4 times higher than that of polyelectrolyte multilayer modified WISPR sensor.

关键词： Nanoporous film Sensing sensitivity biotin Wavelength interrogated surface plasmon resonance

Streptavidin-biotin Complexes as Tools for Modulating an Important DNA Episgenetic Modification

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Chinese Journal of Chemistry 2024年第18期42卷 2166-2172页

作者： Yongjie Liu Xinyan Xu Xingyu Liu Wei Xiong Qianqian Qi Yuanyuan Zhang Jinxuan Hou Tian Tian Xiang Zhoua Key Laboratory of Biomedical Polymers of Ministry of Education College of Chemistry and Molecular SciencesHubei Province Key Laboratory of Allergyand ImmunologyWuhan UniversityWuhanHubei 430072China Department of Thyroid&Breast Surgery Zhongnan Hospital of Wuhan UniversityWuhan UniversityWuhanHubei 430072China

DNA 5-formylcytosine(5fC)is a prominent epigenetic modification within biological *** investigations have shed light on its pivotal role in governing cell fate,gene expression,and disease ***,our comprehension of the precise control of the 5f site structure to influence its functionality remains *** this study,we have successfully achieved precise control over 5fc activity by harnessing the interaction between streptavidin and *** research underscores the potential application of interactions between biomacromolecules and small molecules in advancing the field of DNA epigenetic functional regulation.

关键词： Epigenetic modification biotin Streptavidin Selectivity Regulation

Determination of biotin in Pharmaceutical Formulations by Potassium Permanganate-luminol-CdTe Nanoparticles Chemiluminescence System

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Chemical Research in Chinese Universities 2012年第4期28卷 604-608页

作者： TRAORE Zoumana Sékou SU Xing-guang College of Chemistry Jilin University

A sensitive flow-injection chemiluminescence method was developed for the determination of biotin in the pharmaceutical *** affinity between avidin and biotin was used to adsorb biotin on the polystyrene,with subsequent quantification of biotin based on its ability to enhance the chemiluminescence（CL） signal generated by the redox reaction of potassium permanganate-luminol-CdTe nanoparticles CL *** investigations prove that apart from 3-aminophthalate,the CdTe quantum dots（QDs） play both catalytic and emitter *** optimum conditions,the linear range for the determination of biotin was 0.01―25 ng/mL with a detection limit of 7.3×10-3 ng/mL（S/N=3）.The relative standard deviation of 5 ng/L biotin was 2.06%（n=7）.The proposed method was used to determine the biotin concentration in the pharmaceutical formulations and the recovery was between 96.4% and 104%.The proposed method is simple,convenient,rapid and sensitive.

关键词： Flow-injection Chemiluminescence biotin CdTe nanoparticle

A Simple Method Designed for the Separation of biotinylated Ligate from Free biotin

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Chemical Research in Chinese Universities 1998年第4期14卷 72-74页

作者： LI Tian yu, ZHOU Chang cheng, FANG Rui, QI Jie, ZHOU Hui and LI Wei (Department of Life Science, Jilin University, Changchun, 130023) Jilin Univ Dept Life Sci Changchun 130023 Peoples R China

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关键词： biotinylated Ligate biotin Protein

Total synthesis of dansyl and biotin functionalized ganglioside GM3 by chemoenzymatic method

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Science China Chemistry 2013年第7期56卷 933-938页

作者： SUN Bin JIANG HeYan Research Center of Medicinal Chemistry & Chemical Biology Chongqing Key Laboratory of Catalysis and Functional Organic Molecules Chongqing Technology and Business University

Ganglioside GM3, as well as other gangliosides, offers a variety of modifications in its sialic acid and ceramide moieties GM3 exhibits various types of important biological activities, due to the inability to effectively observe the trafficking of ganglioside GM3, developing sensitive research tools for specific monitoring of GM3 expression and activity is thus desirable. The total synthesis of a dansyl and biotin bifunctionalized fluorescent ganglioside GM3 were reported in this article. From lactose after 13 reaction steps, the compound of 2′ -biotinoylaminoethyl-6-N-dansylamido-6-deoxy-β-D-galatopyranosyl-(1→4)-β-D-glucopy-ranoside was obtained in total yield of 16.2%. Sialylation of dansyl and biotin functionalized lactose by enzymatic method gave dansyl and biotin labeled ganglioside GM3. The fluorescent property of this compound was also investigated.

关键词： fluorescence biotin chemoenzymatic synthesis ganglioside GM3

Study on Interaction of Avidin With biotin Lipid Monolayer

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Chinese Science Bulletin 1993年第6期38卷 486-490页

作者：覃宏谢文章陈浩明隋森芳 Department of Biological Science and Biotechnology Tsinghua Universigy Beijing 100084 PRC Department of Modern Applied Physics Tsinghua University Beijing 100084 PRC. Department of Biological Science and Biotechnology Tsinghua University Beijing 100084 PRC

1 Introduction X-ray diffraction is the major powerful method to resolve the problems on biological macromolecules in detail, but the large single crystals needed for analysis are *** to obtain. In contrast to forming three-dimensional(3D) crystals, many proteins can form two-dimensional (2D) crystals easily. There have been several approaches to making 2D crystals. Lipid layer crystallization is a novel 2D crystallization method developed by Kornberg group. Some water-soluble proteins have been crystallized successfully by this method, and it can be possibly used to crystallize some intrinsic membrane proteins. The formed 2D crystals are

关键词： avidin biotin ATR monolayer.

Intracellular Self-assembly of TPE-biotin Nanoparticles Enables Aggregation-Induced Emission Fluorescence for Cancer-Targeted Imaging

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Chinese Journal of Chemical Physics 2018年第6期31卷 851-856,734页

作者： Yi-fei Xu Jin-hui Jiang Department of Nuclear Medicine The First Aliated Hospital of Anhui Medical University Hefei 230022 China Department of Molecular and Cell Biology University of Leicester Leicester LE1 7RH UK

Fluorogens with aggregation-induced emission (AIE) characteristics have recently been widely applied for studying biological events, and fluorogens with “smart” properties are especially desirable. Herein, we rationally designed and synthesized a biotinylated and reduction-activatable probe (Cys(StBu)-Lys(biotin)-Lys(TPE)-CBT (1)) with AIE properties for cancer-targeted imaging. The biotinylated probe 1 can be actively uptaken by the biotin receptor-overexpressing cancer cells, and then “smartly” self-assemble into nanoparticles inside cells and turn the fluorescence “On”. Employing this “smart” strategy, we successfully applied probe 1 for cancer-targeted imaging. We envision that this biotinylated intelligent probe 1 might be further developed for cancer-targeted imaging in routine clinical studies in the near future.

关键词： Tetraphenylethylene biotin Self-assembly Aggregation-induced emission

Establishment of Cell Free Conversion System With biotin-labelled Recombinant PrP^(sen) Expressed in ***

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Biomedical and Environmental Sciences 2006年第3期19卷 214-218页

作者： JIN ZHANG JIAN-MEI GAO FENG LI JUN HAN LAN CHEN BAO-YUN ZHANG XIAO-FAN WAMG WEI ZHOU YONG LIU XIAO-PING DONG School of Medicine Xi＇an Jiaotong University Zhuque Street 205 Xi ＇an 710061 China State Key Laboratory for Infectious Disease Prevention and Control National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention Yingxin Rd 100 Beijing 100052 China National Laboratory of Medical Molecular Biology Institute of Basic Medical Science Chinese Academy of Medical Sciences and Peking Union Medical College Dongdan Santiao 5 Beijing 100005 China

Objective To report a protocol using biotin-labelled PrP protein in cell free conversion assay instead of isotope. Methods A hamster PrP protein （HaPrP） was expressed in E. coli and purified with HIS-tag affinity chromatograph. After being labelled with biotin, HaPrP was mixed with PrP^sen preparation from scrapie strain 263K. Results Protease-resistant bands were detected after four-day incubation. Conclusion The new conversion model provides a reliable, easily handling, and environment-friendly method for studies of prion and transmissible spongiform encephalopathies.

关键词： Transmissible spongiform eneephalopathies Prion biotin Cell free conversion E. coli

Weak Demand and New Capacity Cause biotin Prices to Dive

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China Chemical Reporter 2009年第23期20卷 20-20页

作者： Zhang Yemei Lycored representative in China

biotin, also named vitamin H, is a water-soluble vitamin product. biotin plays an im-portant role in carbohydrates, protein andfat metabolism. Widely used by industry inanimal feeds, human food andpharmaceuticals.

关键词： biotin vitamin suddenly 鳖洲 metabolism Cause biotin