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Lipid metabolism-related long noncoding RNAs:A potential prognostic biomarker for hepatocellular carcinoma

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World Journal of Gastroenterology 2024年第33期30卷 3799-3802页

作者： Rui-Nan Zhang Jian-Gao Fan Department of Gastroenterology Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of MedicineShanghai 200092China

The incidence rates of hepatocellular carcinoma(HCC)have increased in recent *** advancements in therapy and early diagnosis improving shortterm prognosis,long-term outcomes remain *** noncoding RNAs(lncRNAs)and lipid metabolism play crucial roles in the development and progression of *** lipid synthesis promotes HCC progression,and lncRNAs can reprogram the expression of lipogenic ***,lipid metabolism-related(LMR)-lncRNAs regulate lipid anabolism,accelerating the onset and progression of *** suggests that LMR-lncRNAs could serve as novel prognostic biomarkers and therapeutic targets.

关键词： Long noncoding RNAs Lipid metabolism Hepatocellular carcinoma Prognosis biomarker

Potential role of long noncoding RNA RP5-881L22.5 as a novel biomarker and therapeutic target of colorectal cancer

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World Journal of Gastrointestinal Oncology 2022年第11期14卷 2108-2121页

作者： Hua Zong Jian-Qiang Zou Jian-Peng Huang Shi-Ting Huang Department of Gastrointestinal Surgery The Third People’s Hospital of ShenzhenShenzhen 518000Guangdong ProvinceChina

BACKGROUND The incidence of colorectal cancer in humans is high,and it is in the top five for cancer-related morbidity and *** is one of the main threats to human *** function of long noncoding RNAs in tumor occurrence and development has gradually gained attention in recent *** increasing numbers of studies,researchers have demonstrated that it plays an important role in the pathogenesis of colorectal *** To find out if long noncoding RNA RP5-881L22.5 played a role in the pathogenesis of colorectal cancer in relation to the tumor *** We analyzed the transcriptome data and clinical data in The Cancer Genome Atlas-colon *** CIRBERSORT algorithm was applied to evaluate these tumor-infiltrating immune cells in The Cancer Genome Atlas-colon adenocarcinoma cancer tissue *** the“estimate”package in R,we assessed the tumor immune *** expression level of RP5-881L22.5 in tumor tissue and adjacent normal tissue samples from 4 pairs of colorectal cancer patients was determined by quantitative reverse transcription *** cancer cells were tested for invasiveness using a transwell invasion assay after RP5-881L22.5 expression was knocked *** The expression of lncRNA RP5-881L22.5 was related to the clinical characteristics of the tumors,and it was negatively related to the infiltration level of immune cells in the tumor microenvironment and the expression of T cell inhibitory receptors.A major function of its coexpressed mRNA was to regulate tumor immunity,such as the immune *** quantitative reverse transcription PCR was performed on tumor tissues from 4 pairs of colorectal cancer patients,the results showed that RP5-881L22.5 was highly ***,knocking down the expression of RP5-881L22.5,the invasiveness of colorectal cancer cell lines was reduced,and the apoptosis rate was *** RP5-881L22.5 plays a crucial role in the microenvironm

关键词： Colorectal cancer Long noncoding RNA RP5-881L22.5 Tumor immune microenvironment biomarker Therapeutic target

Phosphoglucose isomerase gene expression as a prognostic biomarker of gastric cancer

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Chinese Journal of Cancer Research 2019年第5期31卷 771-784页

作者： Han-Chen Huang Xian-Zi Wen Hua Xue Run-Sheng Chen Jia-Fu Ji Lei Xu Key Laboratory of RNA Biology Institute of BiophysicsChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China Key Laboratory of Carcinogenesis and Translational Research(Ministry of Education/Beijing) Division of Gastrointestinal Cancer Translational Research LaboratoryPeking University Cancer Hospital&InstituteBeijing 100142China Guangdong Geneway Decoding Bio-Tech Co.Ltd Foshan 528316China Centre for Cognitive Machines and Computational Health(CMaCH) School of Electronic Information and Electrical EngineeringShanghai Jiao Tong UniversityShanghai 200240China Department of Computer Science and Engineering The Chinese University of Hong KongHong Kong 999077China

Objective: Tumor heterogeneity renders identification of suitable biomarkers of gastric cancer(GC)challenging. Here, we aimed to identify prognostic genes of GC using computational ***: We first used microarray technology to profile gene expression of GC and paired nontumor tissues from 198 patients. Based on these profiles and patients’ clinical information, we next identified prognostic genes using novel computational approaches. Phosphoglucose isomerase, also known as glucose-6-phosphate isomerase(GPI), which ranked first among 27 candidate genes, was further investigated by a new analytical tool namely enviro-geno-pheno-state(E-GPS) analysis. Suitability of GPI as a prognostic marker, and its relationship with physiological processes such as metabolism, epithelial-mesenchymal transition(EMT), as well as drug sensitivity were evaluated using both our own and independent public ***: We found that higher expression of GPI in GC correlated with prolonged survival of ***, a combination of CDH2 and GPI expression effectively stratified the outcomes of patients with TNM stage Ⅱ/Ⅲ. Down-regulation of GPI in tumor tissues correlated well with depressed glucose metabolism and fatty acid synthesis, as well as enhanced fatty acid oxidation and creatine metabolism, indicating that GPI represents a suitable marker for increased probability of EMT in GC ***: Our findings strongly suggest that GPI acts as a novel biomarker candidate for GC prognosis,allowing greatly enhanced clinical management of GC patients. The potential metabolic rewiring correlated with GPI also provides new insights into studying the relationship between cancer metabolism and patient survival.

关键词： Gastric cancer gene expression profile prognostic biomarker phosphoglucose isomerase tumor metabolism

Pancreatic cancer serum biomarker PC-594:Diagnostic performance and comparison to CA19-9

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World Journal of Gastroenterology 2015年第21期21卷 6604-6612页

作者： Shawn A Ritchie Bassirou Chitou Qingan Zheng Dushmanthi Jayasinghe Wei Jin Asuka Mochizuki Dayan B Goodenowe Department of biomarker Discovery and Validation Phenome- nome DiscoveriesSaskatoonSK S7N 4L8Canada Department of Manufacturing Phenomenome DiscoveriesSaskatoonSK S7N 4L8Canada Phenomenome Laboratory Services Phenomenome Discoveries

AIM:To investigate serum PC-594 fatty acid levels as a potential biomarker in North American pancreatic cancer(PaC) patients,and to compare its performance to ***:Using tandem mass spectrometry,we evaluated serum PC-594 levels from 84 North American patients with confirmed PaC and 99 cancer-free control *** determined CA19-9 levels by *** between Pa C patients and controls,and association with clinical variables was determined by analysis of variance and *** performance was evaluated by receiver-operator characteristic(ROC)curve analysis,and PC-594 correlation with age and CA19-9 determined by regression ***:Mean PC-594 levels were 3.7 times lower in Pa C patients compared to controls(P 0.05).Using 1.25 μmol/L as a PC-594 threshold produced a relative risk(RR) of 9.4(P biomarker levels are significantly reduced in North American Pa C patients,and showed superior diagnostic performance compared to CA19-9.

关键词： Pancreatic cancer Risk Screening Blood Fatty acid biomarker Metabolite PC-594

Plasma exosomal hsa_circ_0079439 as a novel biomarker for early detection of gastric cancer

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World Journal of Gastroenterology 2023年第22期29卷 3482-3496页

作者： Xiao Li Yan-Li Lin Jia-Kang Shao Xiao-Jie Wu Xiang Li He Yao Fa-Liang Shi Long-Song Li Wen-Gang Zhang Zheng-Yao Chang Ning-Li Chai You-Liang Wang En-Qiang Linghu Department of Gastroenterology The First Medical Center of Chinese PLA General HospitalBeijing 100853China Medical School of Chinese PLA Beijing 100853China Beijing Institute of Biotechnology Beijing 100071China

BACKGROUND Due to the poor prognosis of gastric cancer(GC),early detection methods are urgently *** exosomal circular RNAs(circRNAs)have been suggested as novel biomarkers for *** To identify a novel biomarker for early detection of *** Healthy donors(HDs)and GC patients diagnosed by pathology were *** GC patients and three HDs were selected for exosomal whole-transcriptome RNA *** expression profiles of circRNAs were analyzed by bioinformatics methods and validated by droplet digital polymerase chain *** expression levels and area under receiver operating characteristic curve values of plasma exosomal circRNAs and standard serum biomarkers were used to compare their diagnostic *** There were 303 participants,including 240 GC patients and 63 HDs,involved in the *** expression levels of exosomal hsa_circ_0079439 were significantly higher in GC patients than in HDs(Pbiomarkers were similar between the two *** area under the curve value of exosomal hsa_circ_0079439 was higher than those of standard biomarkers,including carcinoembryonic antigen,carbohydrate antigen(CA)19-9,CA72-4,alpha-fetoprotein,and CA125(0.8595 vs 0.5862,0.5660,0.5360,0.5082,and 0.5018,respectively).The expression levels of exosomal hsa_circ_0079439 were significantly decreased after treatment(Pbiomarker for the diagnosis of GC during both the early and late stages.

关键词： Gastric cancer Exosomes Circular RNA Droplet digital polymerase chain reaction biomarker

Circular RNA circ-LDLRAD3 as a biomarker in diagnosis of pancreatic cancer

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World Journal of Gastroenterology 2017年第47期23卷 8345-8354页

作者： Fan Yang Dong-Yan Liu Jin-Tao Guo Nan Ge Ping Zhu Xiang Liu Sheng Wang Guo-Xin Wang Si-Yu Sun Endoscopy Center Shengjing Hospital of China Medical University Medical Research Center Shengjing Hospital of China Medical University

AIM To analyze the diagnostic value of a circular RNA(circR NA), circ-LDLRAD3, in pancreatic *** Expression levels of circ-LDLRAD3 were tested in both cells and clinical samples; the latter included 30 paired pancreatic cancer tissues and adjacent non-tumorous tissues, 31 plasma samples from patients with pancreatic cancer, and 31 plasma samples from healthy volunteers. Real-time quantitative reverse transcriptionpolymerase chain reaction(q RT-PCR) was performed to measure expression levels of circ-LDLRAD3 in cells and clinical samples; then, the relationship between clinicopathological factors of patient samples and expression of circ-LDLRAD3 in pancreatic cancer was analyzed. The diagnostic value of circ-LDLRAD3 was verified by receiver operating characteristic(ROC) curve *** Circ-LDLRAD3 was up-regulated in pancreatic cancer cell lines(P biomarker in the diagnosis of pancreatic cancer.

关键词： Circular RNA Pancreatic cancer biomarker

hsa-miR-29c and hsa-miR-135b differential expression aspotential biomarker of gastric carcinogenesis

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World Journal of Gastroenterology 2016年第6期22卷 2060-2070页

作者： Amanda Ferreira Vidal Aline MP Cruz Leandro Magalhães Adenilson L Pereira Ana KM Anaissi Nélisson CF Alves Paulo JBS Albuquerque Rommel MR Burbano Samia Demachki Ândrea Ribeiro-dos-Santos Pós-Graduação em Genética e Biologia Molecular Universidade Federal do ParáCidade Universitária Prof.JoséSilveira NettoBelémPA 66075-970Brasil Laboratório de Genética Humana e Médica Instituto de Ciências BiológicasUniversidade Federal do ParáBelémPA 66075-970Brasil Pós-Graduação em Neurociências e Biologia Molecular Universidade Federal do ParáBelémPA 66075-970Brasil Laboratório de Citogenética Humana-Instituto de Ciências Biológicas Universidade Federal do ParáBelémPA 66075-970Brasil Hospital São Camilo e São Luís-Rua Dr.Marcelo Cândia MacapáAP 68901-901Brasil Hospital Universitário João de Barros Barreto Universidade Federal do ParáBelémPA 66075-970Brasil Instituto de Ciências da Saúde Universidade Federal do ParáBelémPA 66075-970Brasil

AIM: To investigate the expression profiles of hsa-mi R-29 c and hsa-mi R-135 b in gastric mucosal samples and their values as gastric carcinogenesis biomarkers. METHODS: The expression levels of hsa-mi R-29 c and hsa-mi R-135 b in normal gastric mucosa, non-atrophic chronic gastritis, intestinal metaplasia and intestinaltype gastric adenocarcinoma were analysed using quantitative real-time PCR. The difference between hsa-mi R-29 c and hsa-mi R-135 b expression profiles in the grouped samples was evaluated by ANOVA and Student's t-test tests. The results were adjusted for multiple testing by using Bonferroni's correction. P values ≤ 0.05 were considered statistically significant. To evaluate hsa-mi R-29 c and hsa-mi R-135 b expressions as potential biomarkers of gastric carcinogenesis, we performed a receiver operating characteristic curve analysis and the derived area under the curve, and a Categorical Principal Components Analysis. In silico identification of the genetic targets of hsa-mi R-29 c and hsa-mi R-135 b was performed using different prediction tools, in order to identify possible genes involved in gastric ***: The expression levels of hsa-mi R-29 c were higher in normal gastric mucosal samples, and decreased progressively in non-atrophic chronic gastritis samples, intestinal metaplasia samples and intestinal-type gastric adenocarcinoma samples. The expression of hsa-mi R-29 c in the gastric lesions showed that non-atrophic gastritis have an intermediate profile to gastric normal mucosa and intestinal-type gastric adenocarcinoma, and that intestinal metaplasia samples presented an expression pattern similar to that in intestinal-type gastric adenocarcinoma. This micro RNA(mi RNA) has a good discriminatory accuracy between normal gastric samples and(1) intestinal-type gastric adenocarcinoma; and(2) intestinal metaplasia, and regulates the DMNT3 A oncogene. hsa-mi R-135 b is up-regulated in non-atrophic chronic gastritis and intestinal m

关键词： Gastric cancer Gastric lesions Micro RNA biomarker Carcinogenesis

n-Alkane bound biomarker fingerprints from asphaltenes in the bitumens of Eastern Dahomey Basin,Nigeria:Source and genetic implications

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Petroleum Research 2024年第1期9卷 85-91页

作者： Modupe A.Egbeola Olubunmi C.Adeigbe Olajide J.Adamolekun Department of Geology University of IbadanIbadanNigeria Structural Geology and Tectonics Institute of Applied GeosciencesKIT-Karlsruhe Institute of TechnologyAdenauerring 20a76131KarlsruheGermany

The asphaltene fractions of the bitumens of Eastern Dahomey Basin in Nigeria,were analyzed by flash pyrolysis-gas chromatography(Py-GC)method in order to unravel its geochemical history and *** distributions of the initial biomarkers of the original oils from the pyrolysates are related to the assessment of organic matter source,paleo-redox conditions and source environment during ***,it effectively establishes the genetic relationship of the *** n-alkane distributions in the pyrolysates reveal nC_(9)-nC_(32)n-alkanes,maximizing at nC_(14),isoprenoids-pristane(Pr)and phytane(Ph),and some n-alkene *** peaks of low to medium-weight nC_(9)-nC20 n-alkanes and low peaks of nC_(21)+n-alkanes characterize the *** reveal that abundant algal organic matter with some terrigenous inputs contributed to the source rock of the *** high concentration of marine organic matter inputs to the source rock is further confirmed by the nC_(17)/nC27 ratios which range from 5.39 to 19.82 and shows the predominance of nC_(17)*** general unimodal n-alkane distributions in the bitumens indicate derivation from similar organic matter types showing that they are genetically *** anoxic to suboxic environmental conditions that prevailed during the deposition of the sediments is revealed by the isoprenoids,Pr/Ph ratios(0.72-1.28).Pristane/nC_(17)and Phytane/nC_(18)range from 0.16 to 0.33 and 0.22 to 0.56,revealing that the bitumens were from predominantly marine organic matter(type II kerogen)preserved in a reducing environment with no evidence of ***,the Ph/nC_(18)ratio and the cross plot of Pr+Ph/nC_(17)+nC_(18)allow the classification of the bitumens into two subfamilies/groups(A and B).The bitumen samples have low wax content as indicated by the degree of waxiness ranging from 0.21 to 0.38 which confirms low terrigenous *** on the carbon preference index(CPI:0.92 to 1.55)and odd-even predo

关键词： Genetic relationship Flash pyrolysis biomarker Asphaltene Pyrolysates

TATA-box-binding protein-associated factor 15 is a novel biomarker that promotes cell proliferation and migration in gastrointestinal stromal tumor

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World Journal of Gastroenterology 2023年第19期29卷 2932-2949页

作者： Cheng-Ming Guo Li Tang Xu Li Liu-Ye Huang Department of Gastroenterology The Affiliated Yantai Yuhuangding Hospital of Qingdao UniversityYantai 264000Shandong ProvinceChina

BACKGROUND Gastrointestinal stromal tumor(GIST)is a common neoplasm with high rates of recurrence and metastasis,and its therapeutic efficacy is still not *** is an unmet need to find new molecular therapeutic targets for ***-boxbinding protein-associated factor 15(TAF15)contributes to the progress of various tumors,while the role and molecular mechanism of TAF15 in GIST progression are still *** To explore new molecular therapeutic targets for GIST and understand the biological role and underlying mechanisms of TAF15 in GIST *** Proteomic analysis was performed to explore the differentially expressed proteins in *** blotting and immunohistochemical analysis were used to verify the expression level of TAF15 in GIST tissues and cell *** counting kit-8,colony formation,wound-healing and transwell assay were executed to detect the ability of TAF15 on cell proliferation,migration and invasion.A xenograft mouse model was applied to explore the role of TAF15 in the progression of *** blotting was used to detect the phosphorylation level and total level of RAF1,MEK and ERK1/*** A total of 1669 proteins were identified as differentially expressed proteins with 762 upregulated and 907 downregulated in ***15 was selected for the further study because of its important role in cell proliferation and ***15 was significantly over expressed in GIST tissues and cell *** of TAF15 was associated with larger tumor size and higher risk stage of ***15 knockdown significantly inhibited the cell proliferation and migration of GIST in vitro and suppressed tumor growth in ***,the inhibition of TAF15 expression significantly decreased the phosphorylation level of RAF1,MEK and ERK1/2 in GIST cells and xenograft tissues,while the total RAF1,MEK and ERK1/2 had no significant *** TAF15 is over expressed in GIST tissues and cell *** of TAF15 was associ

关键词： Gastrointestinal stromal tumor Proteomics TATA-box-binding protein-associated factor 15 biomarker Cell proliferation Cell migration