Can miRNA712＿3p be a promising biomarker for early diagnosis of toxoplasmosis?

作     者：Nermine Mogahed Fawzy Hussein Mogahed Safaa Ibrahim Khedr Rasha Abdelmawla Ghazala Inas Mohamed Masoud 

作者机构：Medical Parasitology Department Faculty of Medicine Alexandria University Medical Biochemistry Department Faculty of Medicine Alexandria University Pharmaceutical Chemistry Faculty of Pharmacy and Drug Manufacturing Pharos University in Alexandria 

出 版 物：《Asian Pacific Journal of Tropical Medicine》 (亚太热带医药杂志（英文版）)

年 卷 期：2018年第11卷第12期

页      面：688-692页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

主　　题：Toxoplasma gondii microRNAs miRNA7123p Biomarker Real-time PCR 

摘      要：Objective: To assess the role of miRNA712_3p as a specific biomarker in early detection of toxoplasmosis in plasma of mice acutely infected with Toxoplasma gondii. Methods: Real-time PCR was used to measure the level of miRNA712_3p in plasma of infected mice. Immunecompetent and immune-suppressed mice were examined, three and five days post-infection. Results: Results revealed significant up-regulation of plasma miRNA712_3p in both immunecompetent and immune-compromised groups in comparison to the control non-infected group. Additionally, an increase in the level of miRNA712_3p was noticed correspondently in the parasite density detected in liver impression smears. Conclusions: miRNA712_3p can be used as a novel biomarker for the detection of Toxoplasma gondii infection in both immunecompetent and immune-compromised host.