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2005全国第二届核酸疫苗研讨会

作者： WU Su-Qi SUN Xiu-Qin ZHANG Jin-Xing ZHEN Feng-Rong (First Institute of Oceanography, the State Oceanic Administration, 6 Xianxialing Road, Qingdao 266061, PR China)

Lymphocystis disease virus has a broad spectrum of hosts in both freshwater and saltwater fish. The disease causes considerable economic loss to fish fanning industries around China. Type species of the virus named as LCDV-1 isolated from a flounder has its genome been completely sequenced. Based on the genomic information of LCDV-1, two gene/ORFs coding for a major capsid protein and a membrane (myristylated) protein were selected and their counterparts in LCDV-cn were amplified and sequenced. After homology verification, we applied an integrated methodology to screen potential antigenic determinants or mimotopes of the proteins. Six mimotopes generated from the bioinformatics analysis were synthesized and tested in a competitive ELISA. One out of the six peptides, which derived form ORF-29 of LCDV-1, showed strong competence with three specific monoclonal antibodies for LCDV-cn. The results indicate that bioinformatics technology may provide a rapid, economic and efficient way in epitope, mimotope screening and mapping.

关键词： bioinformatics analyses synthesized peptides membrane protein LCDV-cn

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MicroRNA expression profiling and bioinformatics analyses of Dengue virus-infected peripheral blood mononuclear cells

MicroRNA expression profiling and bioinformatics analyses of...

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第八届全国免疫学学术大会

作者： YIMING QI YING LI LIN ZHANG JUNQI HUANG Institute of Immunology Zhongshan School of Medicine Sun Yat-sen University and Key Laboratory of Tropical Diseases Control Ministry of Education

Dengue virus(DENV) can cause self–limiting dengue fever(DF) and severe dengue hemorrhagic fever(DHF) and dengue shock syndrome(DSS).It is generally believed that cytokine storm leads to the increased plasma leakage characteristic of DHF/*** this study, peripheral blood mononuclear cells(PBMCs) were isolated from blood samples of healthy volunteers, and infected with DENV serotype 2(DENV2).Culture supernatants of DENV2-infected and uninfected PBMCs were tested against a human cytokine *** 6-12 h post-infection, levels of CCL5, IL-6, IL-8 were remarkably elevated, while those of TNF-α *** RNA isolated from these PBMCs was used against a human miRNA microarray to identify differentially expressed microRNAs(miRNAs).11 upregulated and 4 downregulated miRNAs were valided by q *** analyses of Sanger mibase, miRanda and Target Scan, we found 261 common predicted *** were used to identify homologous sequences on mRNAs of putative target genes that may be directly bound by the miRNAs identified above and we found cytokines and epigenetic regulators might be putative target genes of these miR *** the analyses of Ingenuity Pathway Analysis(IPA), we noticed that canonical pathways like biological regulation pathway which were predicted to be highly modulated by these miRNA

关键词： dengue virus peripheral blood mononuclear cells microRNAs bioinformatics analyses

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Molecular Cloning, Characterization and Sequence Analysis of KCNQ4 in Large Odorous Frog, Odorrana graminea

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Asian Herpetological Research 2019年第4期10卷 211-218页

作者： Ningning LU Ziwen GU Zhuo CHEN Xiaohong CHEN College of Life Sciences Henan Normal University

Acoustic communication is essential for anuran survival and reproduction, and masking background noise can affect the effective acoustic communication. The larger odorous frog(Odorrana graminea) inhabits noise montane streams, and it has shown an ultrasound communication adaptation. However, the molecular mechanism underlying their ultrasonic hearing adaptation remains unknown. To characterize and investigate the molecular characteristics and evolution of the high-frequency hearing-sensitive gene(KCNQ4) in O. graminea, termed as OgKCNQ4, the rapid amplification of cDNA ends(RACE) was performed to amplify the cDNA of OgKCNQ4. Different bioinformatics analyses were used to investigate the molecular characteristics. Multiple nucleotide and amino acid sequence alignment were conducted, and phylogenies were reconstructed under the maximum likelihood and Bayesian approaches. The full-length cDNA of OgKCNQ4 was 2065 bp, and the open reading frame(ORF) was 2046 bp encoding for a putative protein with 681 amino acids. The relative molecular weight of OgKCNQ4 was 76.453 kD and the putative PI was 9.69. Secondary structure prediction analyses suggested 42.29% alpha helixes and 43.76% random coils in OgKCNQ4. Gene homology and Phylogenetic analyses revealed the closest relationship between OgKCNQ4 and KCNQ4 of Nanorana parkeri with 96.9% similarity and 95.0% identity. We first determined the full-length cDNA of OgKCNQ4 and the results here could provide foundations for further study on the evolution of KCNQ4 and its relationship to ultrasonic communication in amphibians.

关键词： Odorrana graminea KCNQ4 cDNA bioinformatics analyses

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