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Experimental Study on Inactivation of bacterial endotoxin by Using Dielectric Barrier Discharge

Plasma Science and Technology 2011年第6期13卷 651-655页

作者：石兴民李娅西张冠军马跃邵先军 Environment and Genes Related to Diseases Key Laboratory of Education Ministry Xi'an Jiaotong University College of Medicine State Key Laboratory of Electrical Insulation and Power Equipment Xi'an Jiaotong University State Grid AC Engineering Construction Company

The low-temperature plasma （LTP） generated by dielectric barrier discharge （DBD） was used to sterilize the *** endotoxin, which is usually difficult to kill by traditional methods. Three different concentrations of bacterial endotoxin （1 EU/mL, 0.5 EU/mL and 0.25 EU/mL） were treated by LTP for different time （20 s, 40 s and 60 s）. Tachypleus amebocyte lysate （TAL） method was employed to detect the concentration variation of bacterial endotoxin before and af- ter the plasma treatment, and endotoxic shock mice model was used to evaluate the inactivation effects of LTP on endotoxin for further study. Experimental results demonstrated that, DBD plasma can inactivate the bacterial endotoxin quickly and effectively, and when the LTP treatment time was increased, the concentrations of bacterial endotoxin decreased gradually （after 60 s plasma treatment, its inactivation effect was beyond the Chinese pharmacopoeia standard）, and the average survival time of mice gradually extended. The possible inactivation mechanisms are proposed to be related to reactive oxygen species （ROSs）.

关键词： dielectric barrier discharge (DBD) low-temperature plasma tachypleus ame-bocyte lysate (TAL) bacterial endotoxin

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A new method for concentration analysis of bacterial endotoxins in perfluorocarbon

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Frontiers of Materials Science 2014年第4期8卷 399-402页

作者： Dan-Dan CHEN Xiao-Ming FENG Chun-Ren WANG Qing-Quan HUANG Zhao-Peng YANG Qing-Yuan MENG National Institutes for Food and Druq Control Beijing 100050 China School of Materials Science and Engineering Tsinghua University Beijing 100084 China

This communication demonstrates the feasibility of the gel-clot method for the analysis of bacterial endotoxins in water extracts of perfluorocarbon which is a water insoluble liquid medical device. Perfluorocarbon （10 mL） was shaken with 10 mL water for 15 min at 2000 r/min and the endotoxin present was extracted to the aqueous phase without interference inhibition/enhancement of the product and the recovery of endotoxin added to perfluorocarbon was determined, A validation study confirmed that endotoxins presented in perfluorocarbon pass over into the aqueous phase at concentrations of 20, 10 and 5EU/mL with recoveries from 86.8% to 96.8%. Therefore, the gel-clot test is suitable for detecting bacterial endotoxins in perfluorocarbon which is a water insoluble medical device.

关键词： perfluorocarbon bacterial endotoxin endotoxin recovery

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Effects of aqueous extract of Notobasis syriaca on lipopolysaccharide-induced inflammation in rats

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Asian Pacific Journal of Tropical Medicine 2018年第1期11卷 48-52页

作者： Abdullatif Azab Ahmad Nassar Jacob Kaplanski Reem Mahajneh Galila Agam Abed N.Azab Triangle Research & Development Center Institute of applied Research Department of Clinical Biochemistry and Pharmacology Faculty of Health SciencesBen-Gurion University of the Negev Department of Nursing Faculty of Health SciencesBen-Gurion University of the Negev

Objective: To investigate the effects of a dry aqueous extract of Notobasis syriaca(N. syriaca)on lipopolysaccharide(LPS)-induced inflammation in rats. Methods: Rats were fed the dried extract [500 mg/(kg·d)] for three consecutive days and then were intraperitoneally injected with LPS(1 mg/kg). Two hours after LPS injection, rats were sacrificed and blood and brain regions were collected. Inflammatory mediators' levels in plasma and homogenates of brain regions were determined by ELISA. Results: Pretreatment with the N. syriaca extract resulted in significant anti-inflammatory effects(P<0.05), including: i) attenuated LPS-induced hypothermia; ii) decreased hypothalamus and hippocampus prostaglandin E_2 levels in the LPStreated rats; and, iii) reduced hypothalamus and hippocampus interleukin-6 and tumor necrosis factor-a levels in the LPS-treated rats. Conclusions: These results suggest that N. syriaca possesses anti-inflammatory properties. Thus, it is possible that long-term consumption of this plant may result in beneficial pharmacological effects.

关键词： bacterial endotoxin Brain Cytokines Hypothermia Natural products Plants

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Prophylactic uses of integrin CD18-βA peptide in a murine polymicrobial peritonitis model

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World Journal of Gastroenterology 2010年第21期16卷 2648-2656页

作者： Kwong-Fai Wong Jana Wo David Ho Ronnie T Poon José M Casasnovas John M Luk Department of Pharmacology and Surgery National University Hospital SystemNational University of SingaporeSingapore 117597Singapore Department of Surgery Queen Mary HospitalPokfulamHKSARHong KongChina Centro Nacional de Biotecnología CSICCampus Universidad Autónoma28049 MadridSpain

AIM:To evaluate the prophylactic properties of integrin CD18-βA peptide in a murine model of abdominal polymicrobial peritonitis and ***:bacterial sepsis was induced in Institute of Cancer Research(ICR) mice by cecal ligation and puncture(CLP) *** mice were then injected with either sterile saline or CD18-βA peptide intraperitoneally at 2 h after surgery,and were sacrificed at 12 and 24 h after *** samples were immediately collected,and analyzed for endotoxin activity and tumor necrosis factor(TNF)-α and interleukin(IL)-*** and liver were studied for CD45+ leukocyte and CD3 mRNA *** expression of intercellular adhesion molecule(ICAM)-1,vascular cell adhesion molecule(VCAM) and E-selectin was also ***:Intraperitoneal injection of CD18-βA peptide significantly suppressed circulating endotoxin activity(Pendotoxin antagonist that can protect surgical patients against sepsis-associated lethality.

关键词： bacterial endotoxin Integrin CD18 Inflammation Biotherapeutic peptide

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Effect of different minimally invasive gastric cancer surgical approaches on postoperative intestinal mucosal barrier function

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Journal of Nutritional Oncology 2024年第3期9卷 98-102页

作者： Kai-Yan Li Dao-Lai Huang Bo Wen Shi-Zhuang Wei Lin-Yi Li Wen-Jie Fang Xiang-Hua Wu Department of Gastrointestinal and Gland Surgery The First Affiliated Hospital of Guangxi Medical UniversityNanning 530021GuangxiChina Department of Gastrointestinal Surgery the Central Hospital of ShaoyangShaoyang 422000HunanChina Department of Emergency Medicine The First People's Hospital of NanningNanning 530022GuangxiChina

Background:The aim of this study was to investigate the effects of different minimally invasive surgical procedures on intestinal muco-sal barrier ***:In this study,76 patients who underwent minimally invasive gastric cancer surgery were selected,and peripheral blood was collected to test the levels of serum plasma D-lactic acid,diamine oxidase,and bacterial endotoxin before and 1 and 3 days after *** markers were compared at different time points before and after surgery to understand the recovery of the intestinal muco-sal barrier function in patients after ***:On the first postoperative day,the change in serum D-lactic acid relative to the preoperative levels was significantly(Pbacterial endotoxin levels were significantly higher on the first postoperative day compared with preoperative levels,and although they showed a gradual decrease by the third day,they remained significantly higher than the pre-operative levels(Pbacterial endotoxin levels between the preoperative sample and the sample collected on the third postoperative day were statistically significant(Pbacterial endotoxin levels were significantly higher in postoperative patients and showed a trend to gradually decrease,suggesting that the intestinal mucosal barrier function of patients after minimally invasive gastric cancer surgery is damaged and t

关键词： Surgical procedures Intestinal mucosal barrier Serum D-lactic acid Diamine oxidase bacterial endotoxin

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