A new method for concentration analysis of bacterial endotoxins in perfluorocarbon
A new method for concentration analysis of bacterial endotoxins in perfluorocarbon作者机构：National Institutes for Food and Druq Control Beijing 100050 China School of Materials Science and Engineering Tsinghua University Beijing 100084 China
出 版 物：《Frontiers of Materials Science》 (材料学前沿（英文版）)
年 卷 期：2014年第8卷第4期
页 面：399-402页
核心收录：
学科分类：1007[医学-药学(可授医学、理学学位)] 100704[医学-药物分析学] 07[理学] 070602[理学-大气物理学与大气环境] 0706[理学-大气科学] 10[医学]
基 金：supported by the Science and Technology Support Program of National 12th Five Year Plan
主 题：perfluorocarbon bacterial endotoxin endotoxin recovery
摘 要：This communication demonstrates the feasibility of the gel-clot method for the analysis of bacterial endotoxins in water extracts of perfluorocarbon which is a water insoluble liquid medical device. Perfluorocarbon （10 mL） was shaken with 10 mL water for 15 min at 2000 r/min and the endotoxin present was extracted to the aqueous phase without interference inhibition/enhancement of the product and the recovery of endotoxin added to perfluorocarbon was determined, A validation study confirmed that endotoxins presented in perfluorocarbon pass over into the aqueous phase at concentrations of 20, 10 and 5EU/mL with recoveries from 86.8% to 96.8%. Therefore, the gel-clot test is suitable for detecting bacterial endotoxins in perfluorocarbon which is a water insoluble medical device.
维普期刊数据库