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Light-Harvesting artificial cells for the Generation of ATP and NADPH

Chinese Journal of Chemistry 2023年第1期41卷 57-63页

作者： Ziwei Su Xiangxiang Zhang Weichen Wang Mingdong Dong Xiaojun Han State Key Laboratory of Urban Water Resource and Environment School of Chemistry and Chemical EngineeringHarbin Institute of Technology92 West Da-Zhi StreetHarbinHeilongjiang 150001China Interdisciplinary Nanoscience Center(iNANO) Aarhus UniversityAarhus DK-8000Denmark

The bottom-up construction of self-powered artificial cells is significant to understand the energy supply and metabolism of nature ***,we demonstrate an efficient manner to build thylakoid-containing artificial cells,which continuously convert light energy into chemical energy to supply adenosine 5'-triphosphate(ATP)under light *** production of ATP supplies energy to promote the biological enzyme cascade reactions,where glucose is transformed into glucose 6-phosphate(G6P)under the catalysis of hexokinase(HK).G6P was further converted to gluconolactone 6-phosphate(PG)in the presence of 6-phosphate dehydrogenase(G6PDH),meanwhile NADP^(+) was converted to nicotinamide adenine dinucleotide phosphate(NADPH).The self-powered artificial cells were demonstrated to generate ATP and NADPH successively,which provided a way for building more complicated artificial cells.

关键词： artificial cells Thylakoids Metabolism Enzymes Vesicles Light harvesting ATPNADPH GUVs

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Vlodeling information exchange between living and artificial cells

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Frontiers of Electrical and Electronic Engineering in China 2017年第1期5卷 76-89页

作者： Keith C.Heyde Mary Joe K. Rice Sung-Ho Paek Felicia Y. Scott Ruihua Zhang Warren C. Ruder Department of Mechanical Engineering Carnegie Mellon University Pittsburgh PA 15213 USA Department of Mechanical Engineering Virginia Polytechnic Institute and State University Blacksburg VA 24061 USA Department of Biological Systems Engi-neering Virginia Polytechnic Institute and State University Black sburg VA 24061 USA Department of Bioengineering University of Pittsburgh Pittsburgh PA 15219 USA

Background： The tools of synthetic biology have enabled researchers to explore multiple scientific phenomena by directly engineering signaling pathways within living cells and artificial protoeells. Here, we explored the potential for engineered living cells themselves to assemble signafing pathways for non-living protocells. This analysis serves as a preliminary investigation into a potential origin of processes that may be utilized by complex living systems. Specifically, we suggest that if living cells can be engineered to direct the assembly of genetic signaling pathways from genetic biomaterials in their environment, then insight can be gained into how naturally occurring living systems might behave similarly. Methods： To this end, we have modeled and simulated a system consisting of engineered cells that control the assembly of DNA monomers on microparticle scaffolds. These DNA monomers encode genetic circuits, and therefore, these microparticles can then be encapsulated with minimal transcription and translation systems to direct protocell phenotype. The modeled system relies on multiple previously established synthetic systems and then links these together to demonstrate system feasibility. Results： In this specific model, engineered cells are induced to synthesize biotin, which competes with biotinylated, circuit-encoding DNA monomers for an avidinized-microparticle scaffold. We demonstrate that multiple synthetic motifs can be controlled in this way and can be tuned by manipulating parameters such as inducer and DNA concentrations. Conclusions： We expect that this system will provide insight into the origin of living systems as well as serve as a tool for engineering living cells that assemble complex biomaterials in their environment.

关键词： synthetic biology artificial cells biotin microparticles

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