Light-Harvesting Artificial Cells for the Generation of ATP and NADPH
作者机构：State Key Laboratory of Urban Water Resource and EnvironmentSchool of Chemistry and Chemical EngineeringHarbin Institute of Technology92 West Da-Zhi StreetHarbinHeilongjiang 150001China Interdisciplinary Nanoscience Center(iNANO)Aarhus UniversityAarhus DK-8000Denmark
出 版 物：《Chinese Journal of Chemistry》 (中国化学（英文版）)
年 卷 期：2023年第41卷第1期
页 面：57-63页
核心收录：
学科分类：08[工学] 09[农学] 0703[理学-化学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种]
基 金：supported by the National Natural Science Foundation of China(Grant Nos.21929401,,22174031,22111540252,21773050) the Fundamental Research Funds forthe Central Universities(HIT.OCEF.2021026) the Heilongjiang Touyan Team(HITTY-20190034) the Natural Science Foundation of Heilongjiang Province(ZD2022B001)
主 题：Artificial cells Thylakoids Metabolism Enzymes Vesicles Light harvesting ATPNADPH GUVs
摘 要：The bottom-up construction of self-powered artificial cells is significant to understand the energy supply and metabolism of nature ***,we demonstrate an efficient manner to build thylakoid-containing artificial cells,which continuously convert light energy into chemical energy to supply adenosine 5 -triphosphate(ATP)under light *** production of ATP supplies energy to promote the biological enzyme cascade reactions,where glucose is transformed into glucose 6-phosphate(G6P)under the catalysis of hexokinase(HK).G6P was further converted to gluconolactone 6-phosphate(PG)in the presence of 6-phosphate dehydrogenase(G6PDH),meanwhile NADP^(+) was converted to nicotinamide adenine dinucleotide phosphate(NADPH).The self-powered artificial cells were demonstrated to generate ATP and NADPH successively,which provided a way for building more complicated artificial cells.
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