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aquaporin 1 overexpression may enhance glioma tumorigenesis by interacting with the transcriptional regulation networks of Foxo4,Maz,and E2F families

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Chinese Neurosurgical Journal 2024年第1期10卷 20-27页

作者： Ying Guan Jinhua Han Die Chen Yuefu Zhan Jianqiang Chen Department of Ultrasound The First Affiliated Hospital of Hainan Medical CollegeHaikou City 570102Hainan ProvinceChina Department of Radiology The First Affiliated Hospital of Hainan Medical CollegeHaikou City 570102Hainan ProvinceChina Department of Radiology Hainan Children’s HospitalHaikou City 571103Hainan ProvinceChina

Background The glioblastoma has served as a valuable experimental model system for investigating the growth and invasive properties of ***-1(AQP1)in facilitating cell migration and potentially contributing to tumor *** this study,we analyzed the role of AQP1 overexpression in glioblastoma and elucidated the main mechanisms *** AQP1 overexpression recombinant vector was introduced into C6 rat glioma cells to construct an AQP1 overexpression C6 cell line,and its effect on cell viability and migration ability was detected by MTT and *** was extracted by Trizol method for gene sequencing and transcriptomics analysis,and the differentially expressed genes(DEGs)were enriched for up-and downregulated genes by Principal component analysis(PCA),and the molecular mechanism of AQP1 overexpression was analyzed in comparison with the control group using the NCBI GEO *** analysis was performed using Mann-Whitney paired two tailed t *** The cell viability of AQP1-transfected cell lines increased by 23%and the mean distance traveled increased by 67%compared with the control *** analysis of gene expression showed that there were 12,121 genes with an average transcripts per million(TPM)value greater than *** accounted for 13%of the genes expressed,with the highest correlation with upregulated genes being FOXO4 and MAZ,and the highest with down-regulated genes being E2F *** AQP1 may be implicated in glioma formation by interacting with the transcriptional regulation networks involving the FOXO4,MAZ,and E2F1/*** findings shed light on the potential significance of AQP1 in glioma pathogenesis and warrant further investigations to unravel the underlying molecular mechanisms.

关键词： C6 cell line aquaporin 1 Gioma Migration

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aquaporin 1 Facilitated Hepatocellular Carcinoma SMMC7221 Cell Migration Associated with Water Permeability

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Chemical Research in Chinese Universities 2011年第1期27卷 99-103页

作者： ZHANG Ai-li LI Jiang WANG Yan-qing ZAKNROU Zohra MA Tong-hui LI Xiao-meng School of Life Sciences Northeast Normal University Changchun 130024 P. R. China Dental Hospital Jilin University Changchun 130021 P. R. China Bo ＇an Brothers Holding Pharmaceutical Co. Ltd. （China） Changchun 130012 P. R. China Central Research Laboratory The Second Hospital of Jilin University Changchun 130041 P R. China

The authors investigated the regulation of human aquaporin I（hAQP1） and the involvement of aquaporin 1 （AQP 1） in the migration of human hepatocellular carcinoma SMMC-7221 cells using RNA intereference technology Firstly, two short hairpin RNA（shRNA） constructs in PBSU6 vector were reconstructed and their knockdown effects were identified in SMMC-7221 cells. Next, the involvement of endogenous hAQP1 in regulating the migration of SMMC-7221 cells was investigated via siRNA technology. HAQPI-shRNA can specifically inhibit AQP1 dependent osmotic water permeability. Meanwhile the migration of SMMC-7221 cells was inhibited remarkably after silencing AQP1 by performing transwell cell migration assay and in vitro wound healing assay. Furthermore, in the presence of an inhibitor HgCl2, the water permeability of the cell membrane was remarkably decreased, the expression of AQP1 was upregulated after HgCla treatment and the cell movement was decreased at the moment. Increased AQP1 cannot attenuate cell migration ability when cell membrane loses its water permeability function. This demonstrates that the cell migration was remarkably related to the transporting water function of cell membrane.

关键词： aquaporin 1 shRNA HgCl2 Cell migration

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GST Fusion Protein Based Specific Polyclonal Antibody Preparation of Mouse aquaporin 1

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Chemical Research in Chinese Universities 2009年第4期25卷 500-505页

作者： LI Jiang YANG Nan-yang GUAN Xin-gang ZHANG Shu-zhi ZHANG Yan QIN Mei-ling MA Tong-hui LI Xiao-meng Dental Hospital Jilin University Changchun 130041 P. R. China Membrane Channel Research Laboratory School of Life Sciences Northeast Normal University Changchun 130024 P. R. China

aquaporins（AQPs） are specific membrane channels for water and other small nonionic *** order to overcome the difficulties to generate the effictive antibody of membrane protein,we selected the cytoplasmic C-terminus of aquaporin 1（AQP1） as an unique *** long C-terminus of mouse AQP1 was overexpressed in the Glutathione S-tansferase Gene Fusion *** the basis of the resonable amounts of soluable membrane protein peptides,we prepared the specific *** pursure this object,we constructed pGEX-4T-1/mAQP1（DNA sequence from 700 to 801 bp） recombinant plasmid and transformed it into Escherichia coli BL21 *** GST-AQP1 C-terminal hydrophilic peptide fusion protein was induced by IPTG and further purified by Glutathione Sepharose 4B to obtain the right size fusion *** we immunized the New Zealand rabbits to prepare the *** purified AQP1 antibody showed high sensitivity by ELISA assay and high specificity by Western blot with AQP1 null mice served as negative ***,we also checked the AQP1 localization in the mouse renal tissues in wild type of mice and AQP1 null mice served as negative *** demonstrated that AQP1 was highly expressed at the descending limb of Henle tube using our purified AQP1 antibody,which was consistent with previous *** successful design and preparation of AQP1 antibody through GST technique is an example as making antibodies against a specific membrane protein.

关键词： aquaporin 1 GST fusion protein Polyclonal antibody Gene knockout mice Membrane protein

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Comment on: Cloning and characterization of porcine aquaporin 1 water channel expressed extensively in the gastrointestinal system

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World Journal of Gastroenterology 2006年第27期12卷 4437-4439页

作者： Ali Mobasheri Molecular Pathogenesis and Connective Tissue Research Groups Department of Veterinary Preclinical Sciences Faculty of Veterinary ScienceUniversity of LiverpoolLiverpoolUnited Kingdom

TO THE EDITOR Sir, I read with great interest the recently published article in the World Journal of Gastroenterology by Jin and co-workers on the cloning and characterization of porcine aquaporin 1 water channel from the pig liver and studies on its expression in the porcine gastrointestinal system. The authors should be congratulated for making this important and valuable contribution to the field of aquaporin biology and porcine gastrointestinal physiology. However, there are a number of unresolved issues and controversies concerning the expression of aquaporins （especially aquaporin 1） in the gastrointestinal system that are worthy of additional comment and discussion by Jin and co-workers.

关键词： aquaporin Water Channel aquaporin 1 Gastro-intestinal system Water transport Glycerol transport Digestion Absorption Secretion

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Correlation between the Expression of aquaporin 1 and Hypoxia-inducible Factor 1 in Breast Cancer Tissues

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Journal of Huazhong University of Science and Technology(Medical Sciences) 2008年第3期28卷 346-348页

作者：殷铁军于世英肖亮张君刘聪卢运萍刘承平 Department of Comprehensive Medicine Tongji HospitalTongji Medical CollegeHuazhong University of Science and Technology Department of Oncology Tongji HospitalTongji Medical CollegeHuazhong University of Science and Technology Department of Pathology Tongji HospitalTongji Medical CollegeHuazhong University of Science and Technology Department of Obstetrics and Gynecology Tongji HospitalTongji Medical CollegeHuazhong University of Science and Technology Department of Mathmatics Huazhong University of Agriculture

The correlation between aquaporin 1 （AQP1） and hypoxia-inducible factor 1 （HIF 1） in breast cancer tissues was preliminarily studied. In 155 cases of breast cancer, the expression levels of AQP1 were detected by immunohistochemisty in HIFl-positive group or HIFl-negative group, and the correlation between AQP1 and HIF1 was analyzed. The overexpression of AQP1 and HIF1 were observed in 155 cases of breast cancer tissues. The expression level of AQP1 in HIFl-positive group was significantly higher than that in HIFl-negative group. The positive expression rate of AQP1 was 296.55±24.67 and 168.37±37.53 in HIFl-positive group and HIFl-negative group respectively with the difference being very significant between them （P〈0.001）. It was concluded that AQP1 was overexpressed in the HIFl-positive group and there were some correlations between AQP1 and HIF1, suggesting they interact each other and regulate the oncogenesis of breast cancer.

关键词： aquaporin 1 hypoxia-inducible factor 1 breast cancer correlation

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Shen Qi Wan attenuates renal interstitial fibrosis through upregulating AQP1

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Chinese Journal of Natural Medicines 2023年第5期21卷 359-370页

作者： LIN Yiyou WEI Jiale ZHANG Yehui HUANG Junhao WANG Sichen LUO Qihan YU Hongxia JI Liting ZHOU Xiaojie LI Changyu School of Pharmaceutical Sciences Zhejiang Chinese Medical UniversityHangzhou 310053China Academy of Chinese Medical Sciences Zhejiang Chinese Medical UniversityHangzhou 310053China

Renal interstitial fibrosis(RIF)is the crucial pathway in chronic kidney disease(CKD)leading to the end-stage renal ***,the underlying mechanism of Shen Qi Wan(SQW)on RIF is not fully *** the current study,we investigated the role of aquaporin 1(AQP1)in SQW on tubular epithelial-to-mesenchymal transition(EMT).A RIF mouse model induced by adenine and a TGF-β1-stimulated HK-2 cell model were etablished to explore the involvement of AQP 1 in the protective effect of SQW on EMT in vitro and in ***,the molecular mechanism of SQW on EMT was explored in HK-2 cells with AQP1 *** results indicated that SQW alleviated kidney injury and renal collagen deposition in the kidneys of mice induced by adenine,increased the protein expression of E-cadherin and AQP1 expression,and decreased the expression of vimentin andα-smooth muscle actin(α-SMA).Similarly,treatmement with SQW-containing serum significantly halted EMT process in TGF-β1 stimulated HK-2 *** expression of snail and slug was significantly upregulated in HK-2 cells after knockdown of ***1 knockdown also increased the mRNA expression of vimentin andα-SMA,and decreased the expression of *** protein expression of vimentin increased,while the expression of E-cadherin and CK-18 significantly decreased after AQP1 knockdown in HK-2 *** results revealed that AQP1 knockdown promoted ***,AQP1 knockdown abolished the protective effect of SQW-containing serum on EMT in HK-2 *** sum,SQW attentuates EMT process in RIF through upregulation of the expression of AQP1.

关键词： Shen Qi Wan Chronic kidney disease Renal interstitial fibrosis Epithelial to mesenchymal transition aquaporin 1

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Decreased expression of AQP1 and AQP5 in acute injured lungs in rats

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Chinese Medical Journal 2002年第7期115卷 963-967,141页

作者：焦光宇李尔然于润江中国医科大学第一临床学院呼吸疾病研究所沈阳110001

OBJECTIVE: To determine if aquaporin1 (AQP1) and aquaporin5 (AQP5) are expressed in the alveolar capillary membrane in rats. Moreover, to investigate the alteration of AQP1 and AQP5 in acute injured lungs. METHODS: The distribution of AQP1 and AQP5 in alveolar capillary membrane were investigated by immunohistochemistry and immunoelectron microscopy with affinity-purified antibodies to human AQP1 and AQP5. To study the possibility that alveolar capillary membrane AQP1 and AQP5 undergo altered regulation, we established a rat model using alveolar instillation of lipopolysaccharide (LPS). RESULTS: Immunolabelling showed AQP1 was stained primarily in the microvascular endotheli a of normal lungs, while AQP5 was expressed in type I pneumocytes. Immunohisto chemical analysis showed a significant decrease in the expression of AQP1 and AQP5 in injured lungs at 4h-48h after LPS instillation. AQP1 protein was resumed partly at 24h after LPS instillation and steroid administration, whereas AQP5 was unchanged. CONCLUSION: The decreased expressions of AQP1 and AQP5 in injured lungs suggest that both of them may play a role in abnormal fluid transportation.

关键词： Membrane Proteins Animals aquaporin 1 aquaporin 5 aquaporins Immunohistochemistry Lipopolysaccharides Lung Male Microscopy, Immunoelectron Rats Rats, Sprague-Dawley Respiratory Distress Syndrome, Adult

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Expression and role of AQP1 in cervical squamous carcinoma and its precancerous lesions

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Journal of Medical Colleges of PLA(China) 2008年第4期23卷 237-242页

作者： Liu Ming Sun Ying Zhang Jian Wang Rong Gao Ya Department of Gynecology and Obstetrics Second Affiliated Hospital Xi ＇an Jiaotong University Xi＇an 710004 China Department of Pathology First Affiliated Hospital Medical College Xi＇an Jiaotong University Xi＇an 710061 China

Objective： To investigate the expression of aquaporin 1 in cervical squamous carcinomas （CSC） and cervical precancerous lesions, and the relationship between the tumor clinicopathological parameters, prognosis and the expression of AQP1. Methods： Immunohistochemical method （EliVision） was used to detect the expression of AQP1 in samples from 106 patients [20 with normal cervical tissue, 30 with cervical intraepithelial neoplasia （stage Ⅰ and Ⅱ） and 56 with CSC]. Survival analysis was performed by Kaplan-Meier method. Results： AQP1 protein was expressed in vascular endothelia of all samples. It showed upregulation of AQP1 expression in CSC. There was a significant difference between CSC and normal cervical tissues （P〈0.05）. AQP1 was expressed in some tumor cells and unexpressed in normal squamous epithelial cells. And APQl-expressing tumor cells were positively related to lymph node metastasis. Patients with APQl-expressing tumor cells had the lower survival rate than the ones without. Conclusion： Abnormal expression of AQP1 plays an important role in the development of CSC. Positive expression of AQP1 in tumor cells maybe enhances tumor metastasis and could be used as a marker for tumor prognosis.

关键词： aquaporin 1 Cervical squamous carcinoma Immunohistochemistry

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AQP1 is involved in glutamate induced nuclear swelling in astrocytes

AQP1 is involved in glutamate induced nuclear swelling in as...

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中国神经科学学会第十届全国学术会议

作者： Feng JIANG Rui Chao CHAI Ann Yuen Kwan WONG Chun Zhang YANG Ye ZHOU Albert Cheung Hoi YU Neuroscience Research Institute Key Laboratory for Neuroscience(Ministry of Education)Key Laboratory for Neuroscience(National Health and Family Planning Commission)Department of NeurobiologySchool of Basic Medical SciencesHealth Science CenterPeking University Laboratory of Translational Medicine Institute of Systems BiomedicinePeking University

Glutamate(Glu),the most important excitatory neurotransmitter in the central nervous systems(CNS),mediates synaptic plasticity,learning and *** astrocytes being the absorption sink of excess Glu,the intracellular metabolism of Glu is not fully *** performed H-Glu and immunostaining experiments and demonstrated that internalized glutamate in astrocytes could enter the nuclei,and directly trigger nuclear swelling and gene *** swelling was found to require aquaporin 1(AQP1),a water channel protein expressed in the cytoplasm and cell membrane of many cell types where they play important physiological *** this study,AQP1 was detected specifically on astrocytic nuclear envelop both in vivo and in ***-expression of AQP1 in astrocytes enhanced Glu-induced nuclear swelling,while its inhibition or knock-down by shRNA prevented Glu-induced nuclear *** specialization we found might uncover more potential function of Glu in astrocytes that involves AQPl localization on astrocytes nuclear membrane.

关键词： aquaporin 1 glutamate astrocyte nuclear swelling

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