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第一届国际生物防治与生物技术研讨会

作者： K.X.Gao~1 X.G.Liu~(1*) C.Li~2 Q.H.Wang~2 B.L.He~3 and Z.M.Yan~1 1 Department of Phytopathology Shandong Agricultural University Tai’an 271018 P.R.China 2 Guanghuasi tree Farm in Mountain Culai of Tai’an City Tai’an 271000 P.R. China 3 College of Science and Technology Shandong Agricultural University Tai’an 271018 P.R.China

＜正＞The biocontrol potential of Chaetomium spirale ND35 against the Valsa canker of apple tree was preliminarily *** results of dual culture on PDA plate showed *** ND35 showed strong antagonism against Valsa ceratosperma;the inhibition zone in the interaction area of the two colonies of *** and *** ND35 was observed;the crude extract of culture in Corn Steep Powder Broth was able to inhibit the mycelial growth of *** more effectively than that one in MEB（Malt Extract Broth）.The results of biocontrol in greenhouse and in the field indicated that the disease incidence of apple tree of the treatment with *** ND35 was lower significantly than other treatments,including treatment with *** alone,scald and healthy *** reisolation experiment suggested that C. spirale ND35 could colonize in stems and branches of apple trees successfully and the ND35 colonization rate of the treatment with solid wheat bran culture was at higher level than the corn steep powder *** the biocontrol effect in the field of liquid culture was better than the solid culture.

关键词： Chaetomium spirale apple tree canker antagonism biocontrol

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Bio-control trials of Chaetomium spirale ND35 against apple canker

引用

Journal of Forestry Research 2005年第2期16卷 121-124页

作者： XINYa-fen SHANGJin-jie ShanghaiVocationalTechnicalCollegeofAgriculture＆Forestry2ZhongshanRoad SongjiangShanghai.201600P.R.China DongvingForestryBureauDongyingCity25700 ShandongProvinceP.R.China

A new endophytic antagonistic fungus, Chaetomium spirale ND35 from Populus tomentosa, was reported. The bio-control trials of C. spirale ND35 against the Valsa canker of apple were preliminarily investigated. The results of dual culture on PDA plate showed that C. spirale ND35 was capable of strong antagonism against Valsa ceratosperma, and for inhibiting the mycelial growth of V. ceratosperma,.the crude extract of liquid culture of corn steep powder broth was more effective than that one of malt extract broth (MEB). The results of bio-control in greenhouse and field indicated that the disease incidence of apple tree treated with C. spirale ND35 was lower significantly than that treated by other methods. The re-isolation experiment suggested that C. spirale ND35 could colonize in stems and branches of apple trees successfully, and the ND35 colonization rate of the treatment with solid wheat bran culture was higher than that of corn steep powder broth, but the field experiment result the control effect of liquid culture of C. spirale ND35 was better than that of solid culture.

关键词： chaetomium spirale apple tree canker antagonism bio-control valsaceratosperma

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