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Science China Chemistry 1995年第5期38卷 580-589页

作者：赵晓航王秀琴周传农彭仁玲阎水中吴旻 (State Key Laboratory of Molecular Oncology Department of Cell Biology Cancer Institute Chinese Academy of Medical Sciences Peking Union Medical College Beijing 100021 China)

A human esophageal cancer cell line (EC8712) expressing high-level Myc protein was infected with recombmant retroviral particles (pA-BD9) at a multiplicity of infection (MOI) 1:1. This viral particle contains a neomycin-resistant gene and a 1.53-kb antisense RNA spanning the 2nd exon and its flanking sequences of the human c-myc oncogene. The G418-resistant EC8712 clones showed an 86% inhibition of growth rate and morphological changes characteristic of terminal differentiation and apoptosis. A decrease of about 80% of Myc protein was also observed in these infected cells by ABC-ELISA assay. 12-24 h after the infection of ECS712 cells with pA-BD9 at a high viral particle concentration (MOI = 1:10), the integration of the extrinsic 1.53-kb antisense c-myc fragment into the cancer cell genome was evidenced by the Southern blot analysis. Northern blot analyses showed the expression of this antisense fragment and a decrease of the intrinsic c-myc expression by 74% in comparison with that of the parental

关键词： c-myc gene antisense RNA esophageal cancer differentiation apoptosis.

INHIBITED NEOPLASTIC PHENOTYPE BY THE c-Ha-ras antisense RNA

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Science China Chemistry 1991年第12期34卷 1485-1491页

作者：路桂荣武纯静柯杨熊辛李延钧刘景梅许毅胥薇李吉友邓国仁鄂征 Beijing Institute for Cancer Research Beijing 100034 PRC

A 2.0 kb fragment DNA plasmid which expresses antisense to the upstream first exon of c-Ha-ras oncogene was transfected into Ha-ras transformed cell lines, GCM-3T3 and REF-4.3. The transfection leads to the inhibition of malignant behaviour, shown by decreasing of growth speed, colony forming ability on soft agar, tumorigenicity in nude mice and increasing ot differentiation degree. In GCM-3T3 cells the lung metastasis frequency became much less (from 60% to 12.5%) after the transfection and the expression of ras oncogene product, and p21 protein was obviously decreased in the transfected cells. This work has first shown the inhibitory effect of antisense RNA on neoplastic behaviour in China.

关键词： antisense RNA malignant cell reversion c-Ha-ras oncogene cell transfection.

Evidence for the antisense transcription in the proviral R29-127 strain of bovine immunodeficiency virus

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Virologica Sinica 2015年第3期30卷 224-227页

作者： Bin Liu Xuechao Zhao Wenyuan Shen Xiaohong Kong Laboratory of Medical Molecular Virology School of Medicine Nankai University Tianjin 300071 China

Dear Editor,In the late stages of the retroviral life cycle,the transcription of viral pre-m RNA is initiated at the 5′long terminal repeat(5′LTR)and terminated at the 3′LTR;the full-length and spliced viral RNAs are transported out of the nucleus and serve as templates for the translation of viral proteins or alternatively as the full-length viral genome to be packaged into virus particles.

关键词： antisense terminated transcripts nucleus initiated length primer plasmid bovine transfection

Inhibition of Inositol-1-phosphate Synthetase in Mycobacterium Tuberculosis by Chitosan-antisense Nanoparticles

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Journal of Wuhan University of Technology(Materials Science) 2009年第1期24卷 87-90页

作者：李媛媛徐顺清 MOE Key Laboratory School of Public Health Tongji Medical College Huazhong University of Science and Technology

Oligodeoxynucleotides （ODNs） were combined with the biodegradable polymer chitosan to form chitosan-ODN nanoparticles by complex coacervation, in order to improve the sta-bility and intracellular penetration. The diameter of the nanoparticles was light strength size and ranged between 60 and 219 nm with a mean value of 132 nm, while zeta potential was between ＋12 and ＋20 mV at pH 5.5. The chitosan-ODN nanoparticles could partially protect the encapsulated ODN from nuclease degradation. Moreover, chitosan-ODN nanoparticles were much more effective in inhibiting the proliferation of M.tuberculosis than free ODN.

关键词： chitosan antisense oligodeoxynucleotides nanoparticle tuberculosis

Reversal of drug resistance in multidrug-resistant human lung cancer cells by retrovirus-mediated MDR1 antisense RNA

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Chinese Science Bulletin 1996年第23期41卷 1996-2000页

作者：高振强高志萍 Department of Pathology Beijing Medical University Beijing 100083 China

Human lung cancer is one of the commonest cancers in the world,and its incidenceand mortality are very high.About 50% of the patients with lung cancer can be treatedwith surgery,and another 50% need chemotherapy because of cancer metastasis.Addition-ally the patients already having surgical treatment often need chemotherapy.However,thechemotherapy often loses its function on these patients,especially in non-small-cell

关键词： lung cancer reversal of drug resistance MDR1 antisense RNA retrovirus.

Effect of HSP70 antisense RNA on normal sorghum pollen formation

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Chinese Science Bulletin 1997年第23期42卷 1990-1994页

作者： Chen, JN Fu, HY Lu, ZX Qin, HY Zhang, L Guo, ZB Chinese Acad Sci Genet Inst Beijing 100101 Peoples R China

IT has been found that heat shock proteins (hsp) exist universally in animals, plants, microbesand human beings. Some of hsps possess functions of molecular chaperons, such as hsp60,hsp70 and hsp90, which participate in folding and assembling polypeptides to functional struc-tures and transferring them into suitable sites. They also undertake repairing damaged cellularDNA and nucleolus. Therefore, they are house-keeping proteins and play an importantrole in maintaining normal cellular life and functions and in cellular response to environmentalstresses. There exists high homology in DNA sequence among the hsp of the same molecularweight across species. We have first reported the close relationship between hsps

关键词： heat shock protein antisense RNA male sterility sorghum pollen.

Introduction of antisense waxy gene into main parent lines of indica hybrid rice

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Chinese Science Bulletin 2002年第14期47卷 1192-1197页

作者： CHEN Xiuhua LIU Qiaoquan WANG Zongyang WANG Xingwen CAI Xiuling ZHANG Jingliu GU Minghong Key Laboratory of Biotechnology of Jiangsu Province Yangzhou UniversityYangzhou 225009China Shanghai Institute of Plant Physiology and Ecology Chinese Academy of ScienceShanghai 200032China

Amylose content in rice endosperm is one of the key determinants of rice eating and cooking quality, and the poor quality of indica hybrid rice is closely related to the high amylose level in rice grains. In order to improve the grain quality of the indica hybrid rice by genetic engineering, an antisense fragment of rice waxy gene, driven by the 5’-franking sequences of the rice waxy gene, was successfully introduced into three major parent lines of indica hybrid rice, all contain a high amylose level in the grains, via Agrobacte-rium, and more than 100 hygromycin-resistant plants were regenerated. The analysis of PCR amplification and Southern blots indicated that the T-DNA containing the antisense waxy gene had been integrated into the genome of transgenic rice plants. Most of the primary transgenic rice plants grew normally, and the mature seeds from these transgenic plants were performed for analysis of the amylose content. The results showed that the amylose content in the endosperm of some grains

关键词： transgenic rice indica rice maintain line antisense waxy gene amylose content.

Inhibition of hepatitis C virus-transfected cholangiocarcinoma by antisense oligodeoxynucleotide in nude mice

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Hepatobiliary & Pancreatic Diseases International 2004年第1期3卷 115-119页

作者： XiaoFang Liu XianTing Zhou ShengQuan Zou Department of Hepatobiliary Surgery Affiliated Yantai Yuhuangding Hospital Qingdao University Medical College Yantai 264000 China. liu634@***

BACKGROUND:The inhibitory effect of antisense oligodeoxynucleotide (asODN) on the replication and expression of hepatitis C virus (HCV) in vitro is not well elucidated. This study was to assess the effect of asODN on HCV in cholangiocarcinoma.;METHODS:The QBC939 cells transfected by a recombinant HCV containing HCV core gene cloned in vector of PBK-CMV (PBK-HCVC) were treated by 14-mers phosphorothioate ODN complementary to the HCV core genomic region. The variation of HCVmRNA level was detected by RT-PCR. Moreover, the inhibitory effect of asODN was observed in nude mice.;RESULTS:HCVmRNA was detected in transfected-QBC939 cells. The 14-mers complementary phosphorothioate ODN showed effective inhibition on HCVmRNA and unexpression HCVmRNA at 6 micromol/L. The tumorigenicity of the transfected-QBC939 cells incubated with asODN in nude mice was greatly inhibited.;CONCLUSIONS:The results suggest a potential therapy of asODN for HCV infected cholangiocarcinoma.

关键词： cholangiocarcinoma hepatitis C virus core gene HCVmRNA antisense oligodeoxynucleotide nude mice

Inhibitory effect of IGF-Ⅱ antisense RNA on malignant phenotype of hepatocellular carcinoma

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World Journal of Gastroenterology 2000年第2期6卷 266-267页

作者： Dong Hua Yang Ming Qing Zhang Jiang Du Chong Xu Oiao Ming Liang Ji Fang Mao Han Rong Qin Zi Rong Fan Department of Gastroenterology,Zhujiang Hospital,the First Military Medical University,Guangzhou 510282,China Laboratory of Molecular Biology,Zhujiang Hospital,the First Military Medical University,Guangzhou,China Departrnent of Biochemistry,the Second Military Medical University,Shanghai,China 第一军医大学广东广州

INIRODUCTIONAccording to the therapeutic effect and strategy ofantisense RNA for hepatoccllular carcinoma(HCC),we have specifically synthesized partialcDNA of human insulin-like growth factor Ⅱ(IGF-Ⅱ)and constructed IGF-Ⅱ cDNA antisenseeukaryotic expression vector.The constructedvector was introduced into hepatoma cell lineSMMC-7721 to block the intrinsic IGF-Ⅱexpression.The biological behavior changes ofhepatoma cells were observed.All these

关键词： carcinoma hepatocellular insulin-like growth factor Ⅱ genes suppressor tumor RNA antisense liver neoplasms

Effects of Intrathecally Administerd NaV1.8 antisense Oligonucleotide on the Expression of Sodium Channel mRNA in Dorsal Root Ganglion

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Journal of Huazhong University of Science and Technology(Medical Sciences) 2005年第6期25卷 696-699页

作者：刘甬民姚尚龙宋文阁王月兰刘东曾涟 Department of Anesthesiology Union Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430022 China Department of Pain Clinic Shandong Provincial Hospital Jinan 250021 China

Neuropathic pain has been hypothesized to be the result of aberrant expression and function of sodium channels at the site of injury. To investigate the effects of NaV1.8 antisense oligonucleotide on the expression of sodium channel mRNA in dorsal root ganglion （DRG） neurons in chronic neuropathic pain. 24 Sprague-Dawley rats weighing 200--260 g were anesthetized with the intraperitoneal injection of 300 mg· kg^-1 choral hydrate. The CCI model was made by loose ligation of sciatic nerve trunk by 4--0 chromic gut. The mechanical and thermal pain threshold were measured before operation and 1, 3, 5, 7, 9, 11, 13 days after operation. A PE-10 catheter was implanted in subarachnoid space at lumbar region. On the 7th postoperative day the animals were randomly divided into 4 groups. The drugs were injected intrathecally twice a day for 5 consecutive days in group 2--4. The animals were decapitated 14 days after the surgery. The L4--L6 DRG of the operated side was removed and crushed, and total RNA was extracted with Trizol reagent. The contralateral side was used as control. The change of NaV1.8 sodium channel transcripts was determined by RT-PCR. Pain threshold was significantly lowered after CCI as compared with that in control group and was elevated 3 days after antisense oligonucleotide injection. Sensory neuron specific TTX-R sodium channel NaV1.8 transcript was down-regulated after antisense oligonucleotide injection at the dosage of 45 μg as compared with that in CCI group （P〈0.01）, and it was even greater at the dosage of 90 μg. The intrathecally injected NaV1.8 antisense oligonucleotide can reduce the mechanical allodynia and thermal hyperalgesia partially by downregulating the SNS transcript expression.

关键词： tetrodotoxin-resistant sodium channel current neuropathic pain antisense dorsal root ganglion sensory neurons voltage sensing sodium channel type 1.8 （NaV1.8）