Inhibition of Inositol-1-phosphate Synthetase in Mycobacterium Tuberculosis by Chitosan-antisense Nanoparticles

作     者：李媛媛 徐顺清 LI Yuanyuan1, CHEN Zhifei2, ZHANG Hongling1, LI Xiaobo1, SHEN Jie1, LU Shi3, XU Shunqing1(1. MOE Key Laboratory, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China 2. Wuhan Tuberculosis Hospital, Baofeng Road, Wuhan 430030, China;3. Union Hospital, Huazhong University of Science and Technology, Wuhan 430022, China)

作者机构：MOE Key Laboratory School of Public Health Tongji Medical College Huazhong University of Science and Technology 

出 版 物：《Journal of Wuhan University of Technology(Materials Science)》 (武汉理工大学学报（材料科学英文版）)

年 卷 期：2009年第24卷第1期

页      面：87-90页

核心收录：

学科分类：07[理学] 070205[理学-凝聚态物理] 0805[工学-材料科学与工程（可授工学、理学学位）] 0702[理学-物理学] 

基　　金：Funded by the National Natural Science Foundation of China (No. 30170051 and No. 30328031) 

主　　题：chitosan antisense oligodeoxynucleotides nanoparticle tuberculosis 

摘      要：Oligodeoxynucleotides （ODNs） were combined with the biodegradable polymer chitosan to form chitosan-ODN nanoparticles by complex coacervation, in order to improve the sta-bility and intracellular penetration. The diameter of the nanoparticles was light strength size and ranged between 60 and 219 nm with a mean value of 132 nm, while zeta potential was between ＋12 and ＋20 mV at pH 5.5. The chitosan-ODN nanoparticles could partially protect the encapsulated ODN from nuclease degradation. Moreover, chitosan-ODN nanoparticles were much more effective in inhibiting the proliferation of M.tuberculosis than free ODN.