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Relationship between the high-risk HPV infection and the expression of oncogenes, anti-oncogenes in cervical dysplasia

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Journal of Hainan Medical University 2017年第1期23卷 16-19页

作者： Li-Ping Shi Xiu-Jie Sheng Luohu People's Hospital ShenzhenShenzhen518000China Third Affiliated Hospital of Guangzhou Medical University Guangzhou510150China

Objective:To study the relationship between the infection of high-risk HPV in cervical precancerous lesion and the expression of oncogene, ***:218 cases ofcervical intraepithelial neoplasia patients in our hospital during May 2014–May 2016 were chosed and divided into high-risk HPV group (n=107), low-risk HPV group (n=111) according to cervical tissue HPV test;another 100 cases of patients received cervical biopsy and confirmed as benign lesions were enrolled in the control group. RT-PCR method was used to detect the mRNA expression of proto-oncogene and anti-oncogene in three groups, Western-blot method was used to detect the protein expression of Sox-2 and Wnt/β-catenin signal ***: mRNA expression of oncogene DEK, Bmi-1, c-fos, K-ras, Prdx4 in high-risk HPV group were higher than low-risk HPV group and control group (Panti-oncogene P27, P16, DAPK, PTEN, eIF4E3 in high-risk HPV group were lower than low-risk HPV group and control group (Poncogenes and reduce the expression of anti-oncogenes in cervical dysplasia tissues on Sox-2- and Wnt/β-catenin signaling pathway manners.

关键词： Cervical cancer Precancerous lesion High-risk HPV oncogene anti-oncogene

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Reduced expression and genetic alterations of ING4 in human stomach adencarcinoma

Reduced expression and genetic alterations of ING4 in human ...

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中国遗传学会第八次代表大会暨学术讨论会

作者：李明傅松滨 Laboratory of Medical Genetics Harbin Medical University Bio-pharmaceutical Key Laboratory of Heilongjiang Province

As a member of ING gene family which harbor the PHD domain and NLS domain,ING4 was reported to interact with tumor suppressor p53 and found to be involved in various biological activities,including cell cycle arrest,regulation of gene transcription,DNA repair and apoptosis. ING41 was located in nucleus whereas other three variants were located in *** clarify the role of ING4 in human stomach adencarcinoma,we first compared ING4 expression in four human gastric adenocarcinoma cell lines by RT-PCR and Real-time *** ING4 mRNA level was found in all cell lines,while there was lower ING4 expression found in poorly differentiated cell ***,forty samples of human stomach adenocarcinoma tissues and matched no-tumor tissues were investigated using RT-PCR and Real-time ***4 expression level was significantly decreased in stomach adenocarcinoma tissues(P<0.05)and markedly related to gender and infiltration(P<0.05).Then,expression of ING4 was analyzed among other forty human stomach adenocarcinoma samples without matched normal tissues and forty human stomach adenocarcinoma samples with matched normal tissues through tissue microarray *** results indicated that ING4 was located in the nucleus and cytoplasm,especially and interestingly in the parietal *** expression of ING4 was reduced greatly in stomach adenocarcinoma tissues compared with normal stomach tissues(P<0.01),and markedly related to gender(P<0.05).Finally, mutation analysis of this gene was carried out in four human gastric adenocarcinoma cell lines and nine random tumor samples using clone *** data demonstrated complete and correct ORF sequences of ING41,ING42 and ING44,and various mutations in splice variants ING41 and ING42 as *** these findings suggested that the inhibition of ING4 gene expression and mutations of ING4 might be involved in the initiation and proceeding of stomach adenocarcinoma.

关键词： Stomach adenocarcinoma anti-oncogene ING4 expression mutation

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Reduced Expression and Aberrant Splicing of ING4 in Human Stomach Adencarcinoma

Reduced Expression and Aberrant Splicing of ING4 in Human St...

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第八次全国医学遗传学学术会议(中华医学会2009年医学遗传学年会)

作者： LI Ming,JIN Yan,SUN Wen-jing YU Yang~1,BAI Jing~1,CHEN Feng~1,FU Song-bin~(1,2)(1.Laboratory of Medical Genetics,Harbin Medical University,Harbin 150081,China 2.Bio-pharmaceutical Key Laboratory of Heilongjiang Province,Harbin 150081,China)

As a member of ING gene family which harbor the PHD domain and NLS domain,ING4 was reported to interact with tumor suppressor p53 and found to be involved in various biological activities,including cell cycle arrest,regulation of gene transcription, DNA repair and ***41 was located in nucleus whereas other three variants were located in *** clarify the role of ING4 in human stomach adencarcinoma,we first compared ING4 expression in four human gastric adenocarcinoma cell lines by RT-PCR and Real-time *** ING4 mRNA level was found in all cell lines,while there was lower ING4 expression found in poorly differentiated cell ***,forty samples of human stomach adenocarcinoma tissues and matched no-tumor tissues were investigated using RT-PCR and Real-time ***4 expression level was significantly decreased in stomach adenocarcinoma tissues(P<0.05)and markedly related to gender and infiltration(P<0.05).Then,expression of ING4 was analyzed among other forty human stomach adenocarcinoma samples without matched normal tissues and forty human stomach adenocarcinoma samples with matched normal tissues through tissue microarray *** results indicated that ING4 was located in the nucleus and cytoplasm,especially and interestingly in the parietal *** expression of ING4 was reduced greatly in stomach adenocarcinoma tissues compared with normal stomach tissues(P<0.01),and markedly related to gender(P<0.05).Finally,mutation analysis of this gene was carried out in four human gastric adenocarcinoma cell lines and nine random tumor samples using clone *** data demonstrated complete and correct ORF sequences of ING41,ING42 and ING44,and aberrant splicing based on ING41 and ING42 as well. All these findings suggested that the inhibition of ING4 gene expression and mutations of ING4 might be involved in the initiation and proceeding of stomach adenocarcinoma.

关键词： Stomach adenocarcinoma anti-oncogene ING4 Expression Variant

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Reduced Expression and Genetic Alterations of ING4 in Human Stomach Adencarcinoma

Reduced Expression and Genetic Alterations of ING4 in Human ...

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中国遗传学会“发育、遗传和疾病”研讨会

作者： Ming Li~1 Yan Jin~1 Changlong Guo~1 Jing Bai~1 Feng Chen~1 Songbin Fu~(1,2) 1.Laboratory of Medical Genetics,Harbin Medical University,Harbin,150081,China 2.Bio-pharmaceutical Key Laboratory of Heilongjiang Province,Harbin,150081,China

ING4 is a member oflNG gene family and harbors the PHD domain and NLS ***4 is reported to interact with tumor suppressor p53 and found to be involved in various biological activities, including cell cycle arrest,regulation of gene transcription,DNA repair and apoptosis,ING41 is located in nucleus whereas other three variants are located in *** clarify the role of ING4 in human stomach adencarcinoma,we first compared ING4 expression in four human gastric adenocarcinoma cell lines by RT-PCR and Real-time ***/NG4 mRNA level was found in all cell lines,while there is lower ING4 expression found in differentiated cell ***,fourty samples of human stomach adenocarcinoma tissues and matched no-tumor tissues were investigated using RT-PCR and Real-time PCR. ING4 expression level is significantly decreased in stomach adenocarcinoma tissues（P＜0.05）and markedly related to gender and infiltration（P＜0.05）.Then,Expression of ING4 is analyzed among other fourty human stomach adenocarcinoma samples without matched normal tissues and fourty human stomach adenocarcinoma with matched normal tissues,through tissue microarray analysis ING4 expression was found to locate in the nucleus and cytoplasm,especially and interestingly in the parietal ceil,while expression of ING4 is stronger than cytoplasmic *** expression of ING4 was reduced greatly in stomach adenocarcinoma tissues compared with normal stomach tissues（P＜0.01）,but it has no significant relationship with the clinicopathologicai parameters in patients of stomach *** last,we checked mutations in four human gastric adenocarcinoma cell lines and nine random tumor samples using clone sequence,when we found complete and correct ORF sequences of/NG41,/NG42 and ING44, and various mutations in splice variants ING41 and/NG42 as *** these findings suggest that the inhabitation of ING4 gene expression and mutations of ING4 may be involved in the initiation and proceeding of stomach a

关键词： Stomach adenocarcinoma anti-oncogene ING4

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