Reduced expression and genetic alterations of ING4 in human stomach adencarcinoma
作者单位：Laboratory of Medical GeneticsHarbin Medical University Bio-pharmaceutical Key Laboratory of Heilongjiang Province
会议名称：《中国遗传学会第八次代表大会暨学术讨论会》
会议日期：2008年
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
基 金：国家自然科学基金(编号:30572092) 中国博士后科学基金(编号:20060400833和20060400834) 黑龙江省教育厅项目(编号:10551136) 黑龙江省卫生厅项目(编号:2006-278)
关 键 词：Stomach adenocarcinoma anti-oncogene ING4 expression mutation
摘 要：As a member of ING gene family which harbor the PHD domain and NLS domain,ING4 was reported to interact with tumor suppressor p53 and found to be involved in various biological activities,including cell cycle arrest,regulation of gene transcription,DNA repair and apoptosis. ING41 was located in nucleus whereas other three variants were located in *** clarify the role of ING4 in human stomach adencarcinoma,we first compared ING4 expression in four human gastric adenocarcinoma cell lines by RT-PCR and Real-time *** ING4 mRNA level was found in all cell lines,while there was lower ING4 expression found in poorly differentiated cell ***,forty samples of human stomach adenocarcinoma tissues and matched no-tumor tissues were investigated using RT-PCR and Real-time ***4 expression level was significantly decreased in stomach adenocarcinoma tissues(P0.05)and markedly related to gender and infiltration(P0.05).Then,expression of ING4 was analyzed among other forty human stomach adenocarcinoma samples without matched normal tissues and forty human stomach adenocarcinoma samples with matched normal tissues through tissue microarray *** results indicated that ING4 was located in the nucleus and cytoplasm,especially and interestingly in the parietal *** expression of ING4 was reduced greatly in stomach adenocarcinoma tissues compared with normal stomach tissues(P0.01),and markedly related to gender(P0.05).Finally, mutation analysis of this gene was carried out in four human gastric adenocarcinoma cell lines and nine random tumor samples using clone *** data demonstrated complete and correct ORF sequences of ING41,ING42 and ING44,and various mutations in splice variants ING41 and ING42 as *** these findings suggested that the inhibition of ING4 gene expression and mutations of ING4 might be involved in the initiation and proceeding of stomach adenocarcinoma.