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Biotinylated dextran amine anterograde tracing of the canine corticospinal tract

Neural Regeneration Research 2012年第11期7卷 805-809页

作者： Xiao Han Guangming Lv Huiqun Wu Dafeng Ji Zhou Sun Yaofu Li Lemin Tang Department of Human Anatomy Institute of Neurobiology Jiangsu Key Laboratory of Neuroregeneration Medical School Nantong University Nantong 226001 Jiangsu Province China Department of Medical Informatics Institute of Digital Medicine Medical School Nantong University Nantong 226001 Jiangsu Province China Department of Medical Image Engineering Medical School Nantong University Nantong 226001 Jiangsu Province China

In this study, biotinylated dextran amine （BDA） was microinjected into the left cortical motor area of the canine brain. Fluorescence microscopy results showed that a large amount of BDA-labeled pyramidal cells were visible in the left cortical motor area after injection. In the left medulla oblongata, the BDA-labeled corticospinal tract was evenly distributed, with green fluorescence that had a clear boundary with the surrounding tissue. The BDA-positive corticospinal tract entered into the right lateral funiculus of the spinal cord and descended into the posterior part of the right lateral funiculus, close to the posterior horn, from cervical to sacral segments. There was a small amount of green fluorescence in the sacral segment. The distribution of BDA labeling in the canine central nervous system was consistent with the course of the corticospinal tract. Fluorescence labeling for BDA gradually diminished with time after injection. Our findings indicate that the BDA anterograde tracing technique can be used to visualize the localization and trajectory of the corticospinal tract in the canine central nervous system.

关键词： biotinylated dextran amine corticospinal tract anterograde tracing fluorescence canine

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Chondroitinase ABC treatment of injured spinal cord in rats Evaluation of long-term outcomes

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Neural Regeneration Research 2010年第16期5卷 1238-1242页

作者： Haifeng Yuan Yongli Ding Yueming Song Lihong Hu Zili Wang Hao Liu Limin Liu Quan Gong Tao Li Qingquan Kong Department of Orthopedic Surgery West China Hospital Sichuan University Chengdu 610041 Sichuan Province China Department of Orthopedic Surgery Affiliated Hospital Ningxia Medical University Yinchuan 750004 Ningxia Hui Autonomous Region China Department of Orthopedic Surgery First Affiliated Hospital Henan Chinese Medical College Zhengzhou 450000 Henan Province China

Chondroitin sulfate proteoglycans （CSPGs） which are produced by mature oligodendrocytes and reactive astrocytes can be upregulated after spinal cord injury and contribute to regenerative failure. Chondroitinase ABC （ChABC） digests glycosaminoglycan chains on CSPGs and can thereby overcome CSPG-mediated inhibition. However, many current studies have used an incomplete spinal cord injury model, and examined results after 8-12 weeks of ChABC treatment. In this study, a complete rat spinal cord transection injury model was used to study the long-term effects of ChABC treatment by subarachnoid catheter. Pathology of spinal cord regeneration was compared with control 24 weeks following ChABC treatment using immunohistochemistry and axon tracing techniques. At 24 weeks after injury, neurofilament 200 expression was significantly greater in the ChABC treatment group compared with the transection group. In the ChABC treatment group, axonal growth was demonstrated by a large number of biotinylated dextran amine positive axons caudal to, or past, the epicenter of injury. Biotinylated dextran amine-labeled fibers were found in the proximal end of the spinal cord in the transection alone group. These results confirm that ChABC can promote axon growth, neural regeneration, and repair after spinal cord injury in rats long after the initial injury.

关键词： spinal cord injury chondroitinase ABC morphology treatment biotinylated dextran amine anterograde tracing neural regeneration

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